RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother
We used a non-integrative self-replicating RNA vector to establish four iPSC lines: two iPSC lines from a young male carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 (present at virtual homoplasmic level), and two iPSC lines from his healthy mother (carrying the mutation in only...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122002690 |
| _version_ | 1797985742085947392 |
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| author | Tabea Steiner Annika Zink Marie-Therese Henke Giulia Cecchetto Miriam Buenning Andrea Rossi Markus Schuelke Alessandro Prigione |
| author_facet | Tabea Steiner Annika Zink Marie-Therese Henke Giulia Cecchetto Miriam Buenning Andrea Rossi Markus Schuelke Alessandro Prigione |
| author_sort | Tabea Steiner |
| collection | DOAJ |
| description | We used a non-integrative self-replicating RNA vector to establish four iPSC lines: two iPSC lines from a young male carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 (present at virtual homoplasmic level), and two iPSC lines from his healthy mother (carrying the mutation in only about 4 % of mtDNA copies). All iPSC lines exhibited pluripotency characteristics, were capable to give rise to cells of the three germ layers in vitro, and presented a normal karyotype. The derived iPSC lines retained the MT-ATP6 mutation at levels similar to those observed in the parental fibroblasts. |
| first_indexed | 2024-04-11T07:22:53Z |
| format | Article |
| id | doaj.art-229638deae5746c4ad1ae9c1c32c5774 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-11T07:22:53Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-229638deae5746c4ad1ae9c1c32c57742022-12-22T04:37:09ZengElsevierStem Cell Research1873-50612022-10-0164102920RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy motherTabea Steiner0Annika Zink1Marie-Therese Henke2Giulia Cecchetto3Miriam Buenning4Andrea Rossi5Markus Schuelke6Alessandro Prigione7Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, GermanyDepartment of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, GermanyCharité Universitätsmedizin, Berlin, GermanyDepartment of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, GermanyDepartment of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, GermanyGenome Engineering and Model Development Lab (GEMD), IUF-Leibniz Research Institute for Environmental Medicine, Düsseldorf 40225, GermanyCharité Universitätsmedizin, Berlin, GermanyDepartment of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany; Corresponding author.We used a non-integrative self-replicating RNA vector to establish four iPSC lines: two iPSC lines from a young male carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 (present at virtual homoplasmic level), and two iPSC lines from his healthy mother (carrying the mutation in only about 4 % of mtDNA copies). All iPSC lines exhibited pluripotency characteristics, were capable to give rise to cells of the three germ layers in vitro, and presented a normal karyotype. The derived iPSC lines retained the MT-ATP6 mutation at levels similar to those observed in the parental fibroblasts.http://www.sciencedirect.com/science/article/pii/S1873506122002690 |
| spellingShingle | Tabea Steiner Annika Zink Marie-Therese Henke Giulia Cecchetto Miriam Buenning Andrea Rossi Markus Schuelke Alessandro Prigione RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother Stem Cell Research |
| title | RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother |
| title_full | RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother |
| title_fullStr | RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother |
| title_full_unstemmed | RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother |
| title_short | RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother |
| title_sort | rna based generation of ipscs from a boy carrying the mutation m 9185 t c in the mitochondrial gene mt atp6 and from his healthy mother |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122002690 |
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