Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes
The zygote is the first cell of a multicellular organism. In most angiosperms, the zygote divides asymmetrically to produce an embryo-precursor apical cell and a supporting basal cell. Zygotic division should properly segregate symbiotic organelles, because they cannot be synthesized de novo. In thi...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2020-01-01
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| Series: | Quantitative Plant Biology |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2632882820000041/type/journal_article |
| _version_ | 1811155722197532672 |
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| author | Yusuke Kimata Takumi Higaki Daisuke Kurihara Naoe Ando Hikari Matsumoto Tetsuya Higashiyama Minako Ueda |
| author_facet | Yusuke Kimata Takumi Higaki Daisuke Kurihara Naoe Ando Hikari Matsumoto Tetsuya Higashiyama Minako Ueda |
| author_sort | Yusuke Kimata |
| collection | DOAJ |
| description | The zygote is the first cell of a multicellular organism. In most angiosperms, the zygote divides asymmetrically to produce an embryo-precursor apical cell and a supporting basal cell. Zygotic division should properly segregate symbiotic organelles, because they cannot be synthesized de novo. In this study, we revealed the real-time dynamics of the principle source of ATP biogenesis, mitochondria, in Arabidopsis thaliana zygotes using live-cell observations and image quantifications. In the zygote, the mitochondria formed the extended structure associated with the longitudinal array of actin filaments (F-actins) and were polarly distributed along the apical–basal axis. The mitochondria were then temporally fragmented during zygotic division, and the resulting apical cells inherited mitochondria at higher concentration compared to the basal cells. Further observation of postembryonic organs showed that these mitochondrial behaviours are characteristic of the zygote. Overall, our results showed that the zygote has spatiotemporal regulation that unequally distributes the mitochondria. |
| first_indexed | 2024-04-10T04:38:17Z |
| format | Article |
| id | doaj.art-2298ddbc5e6440da81b628cc288fea64 |
| institution | Directory Open Access Journal |
| issn | 2632-8828 |
| language | English |
| last_indexed | 2024-04-10T04:38:17Z |
| publishDate | 2020-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Quantitative Plant Biology |
| spelling | doaj.art-2298ddbc5e6440da81b628cc288fea642023-03-09T12:43:32ZengCambridge University PressQuantitative Plant Biology2632-88282020-01-01110.1017/qpb.2020.4Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotesYusuke Kimata0Takumi Higaki1Daisuke Kurihara2Naoe Ando3Hikari Matsumoto4Tetsuya Higashiyama5Minako Ueda6https://orcid.org/0000-0002-6892-2744Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan Graduate School of Life Sciences, Tohoku University, Sendai, 980-8578, JapanInternational Research Organization for Advanced Science and Technology (IROAST), Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, JapanInstitute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan JST, PRESTO, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, JapanDivision of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, JapanDivision of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, JapanInstitute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, JapanInstitute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan Graduate School of Life Sciences, Tohoku University, Sendai, 980-8578, Japan Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, JapanThe zygote is the first cell of a multicellular organism. In most angiosperms, the zygote divides asymmetrically to produce an embryo-precursor apical cell and a supporting basal cell. Zygotic division should properly segregate symbiotic organelles, because they cannot be synthesized de novo. In this study, we revealed the real-time dynamics of the principle source of ATP biogenesis, mitochondria, in Arabidopsis thaliana zygotes using live-cell observations and image quantifications. In the zygote, the mitochondria formed the extended structure associated with the longitudinal array of actin filaments (F-actins) and were polarly distributed along the apical–basal axis. The mitochondria were then temporally fragmented during zygotic division, and the resulting apical cells inherited mitochondria at higher concentration compared to the basal cells. Further observation of postembryonic organs showed that these mitochondrial behaviours are characteristic of the zygote. Overall, our results showed that the zygote has spatiotemporal regulation that unequally distributes the mitochondria.https://www.cambridge.org/core/product/identifier/S2632882820000041/type/journal_articleArabidopsis thalianaimage quantificationlive-cell imagingmitochondriazygote |
| spellingShingle | Yusuke Kimata Takumi Higaki Daisuke Kurihara Naoe Ando Hikari Matsumoto Tetsuya Higashiyama Minako Ueda Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes Quantitative Plant Biology Arabidopsis thaliana image quantification live-cell imaging mitochondria zygote |
| title | Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes |
| title_full | Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes |
| title_fullStr | Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes |
| title_full_unstemmed | Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes |
| title_short | Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes |
| title_sort | mitochondrial dynamics and segregation during the asymmetric division of arabidopsis zygotes |
| topic | Arabidopsis thaliana image quantification live-cell imaging mitochondria zygote |
| url | https://www.cambridge.org/core/product/identifier/S2632882820000041/type/journal_article |
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