Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2013-11-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/1697 |
| _version_ | 1797237467422654464 |
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| author | S. N. Kozlov V. B. Nikolaev E. Y. Markov L. V. Mironova L. Y. Urbanovich |
| author_facet | S. N. Kozlov V. B. Nikolaev E. Y. Markov L. V. Mironova L. Y. Urbanovich |
| author_sort | S. N. Kozlov |
| collection | DOAJ |
| description | With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis detected polypeptides with IgG-protease activity both in water-soluble and membrane fractions of the strains with different molecular masses. Differences in IgG-protease activity of subcellular fractions of V. cholerae ompT+ and ompT-strains were determined. |
| first_indexed | 2024-03-08T12:19:40Z |
| format | Article |
| id | doaj.art-229aa0f4b27247949d8557e3db90e1c6 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:20:13Z |
| publishDate | 2013-11-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-229aa0f4b27247949d8557e3db90e1c62024-03-28T07:49:12ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962013-11-01061451481643Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroupsS. N. Kozlov0V. B. Nikolaev1E. Y. Markov2L. V. Mironova3L. Y. Urbanovich4Irkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorIrkutsk Antiplague Research Institute of RospotrebnadzorWith the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis detected polypeptides with IgG-protease activity both in water-soluble and membrane fractions of the strains with different molecular masses. Differences in IgG-protease activity of subcellular fractions of V. cholerae ompT+ and ompT-strains were determined.https://www.actabiomedica.ru/jour/article/view/1697vibrio choleraevibrio choleraeproteasessubcellular fractionenzyme-electrophoresisimmunoglobulins g |
| spellingShingle | S. N. Kozlov V. B. Nikolaev E. Y. Markov L. V. Mironova L. Y. Urbanovich Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups Acta Biomedica Scientifica vibrio cholerae vibrio cholerae proteases subcellular fraction enzyme-electrophoresis immunoglobulins g |
| title | Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
| title_full | Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
| title_fullStr | Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
| title_full_unstemmed | Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
| title_short | Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups |
| title_sort | proteolytic cleavage of immunoglobulins g by subcellular fractions of vibrio cholerae o1 and o139 serougroups |
| topic | vibrio cholerae vibrio cholerae proteases subcellular fraction enzyme-electrophoresis immunoglobulins g |
| url | https://www.actabiomedica.ru/jour/article/view/1697 |
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