Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes
Type 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent restenosis (ISR) in patients with coronary artery disease (CAD). Evidence suggests that B cells play a functional role in the progression of atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in patie...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2021-08-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2021-0091 |
| _version_ | 1818025242848657408 |
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| author | Weng Ruiqiang Liu Sudong Gu Xiaodong Zhong Zhixiong |
| author_facet | Weng Ruiqiang Liu Sudong Gu Xiaodong Zhong Zhixiong |
| author_sort | Weng Ruiqiang |
| collection | DOAJ |
| description | Type 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent restenosis (ISR) in patients with coronary artery disease (CAD). Evidence suggests that B cells play a functional role in the progression of atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in patients with ISR remains unclear. This study aims to profile the BCR repertoire in patients with coronary ISR/T2DM. A total of 21 CAD patients with or without ISR/T2DM were enrolled. PBMCs were isolated and examined for BCR repertoire profiles using DNA-seq. Our results showed that the diversity of amino acid sequences in ISR DM patients was higher than that in ISR −DM patients. The frequencies of 21 V/J paired genes differed between ISR DM and −ISR DM patients, while frequencies of 5 V/J paired genes differed between ISR DM and ISR −DM. The −ISR −DM group presented the highest clonotype overlap rate, while ISR DM patients presented the lowest overlap rate. Our study presented the BCR repertoires in patients with ISR/T2DM. The data suggested different BCR signatures between patients with ISR and T2DM. Further analysis of BCR profiles would enhance understanding of ISR. |
| first_indexed | 2024-12-10T04:13:00Z |
| format | Article |
| id | doaj.art-229ca2f20cee49eba0a632a97b041eab |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-10T04:13:00Z |
| publishDate | 2021-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-229ca2f20cee49eba0a632a97b041eab2022-12-22T02:02:40ZengDe GruyterOpen Life Sciences2391-54122021-08-0116188489810.1515/biol-2021-0091Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetesWeng Ruiqiang0Liu Sudong1Gu Xiaodong2Zhong Zhixiong3Research Experimental Center, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-Sen University, Meizhou 514031, People’s Republic of ChinaResearch Experimental Center, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-Sen University, Meizhou 514031, People’s Republic of ChinaResearch Experimental Center, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-Sen University, Meizhou 514031, People’s Republic of ChinaGuangdong Provincial Engineering and Technological Research Center for Molecular Diagnostics of Cardiovascular Diseases, Meizhou 514031, People’s Republic of ChinaType 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent restenosis (ISR) in patients with coronary artery disease (CAD). Evidence suggests that B cells play a functional role in the progression of atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in patients with ISR remains unclear. This study aims to profile the BCR repertoire in patients with coronary ISR/T2DM. A total of 21 CAD patients with or without ISR/T2DM were enrolled. PBMCs were isolated and examined for BCR repertoire profiles using DNA-seq. Our results showed that the diversity of amino acid sequences in ISR DM patients was higher than that in ISR −DM patients. The frequencies of 21 V/J paired genes differed between ISR DM and −ISR DM patients, while frequencies of 5 V/J paired genes differed between ISR DM and ISR −DM. The −ISR −DM group presented the highest clonotype overlap rate, while ISR DM patients presented the lowest overlap rate. Our study presented the BCR repertoires in patients with ISR/T2DM. The data suggested different BCR signatures between patients with ISR and T2DM. Further analysis of BCR profiles would enhance understanding of ISR.https://doi.org/10.1515/biol-2021-0091b cell receptor repertoirerepertoire sequencingin-stent restenosistype 2 diabetes mellitus |
| spellingShingle | Weng Ruiqiang Liu Sudong Gu Xiaodong Zhong Zhixiong Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes Open Life Sciences b cell receptor repertoire repertoire sequencing in-stent restenosis type 2 diabetes mellitus |
| title | Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes |
| title_full | Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes |
| title_fullStr | Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes |
| title_full_unstemmed | Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes |
| title_short | Clonal diversity of the B cell receptor repertoire in patients with coronary in-stent restenosis and type 2 diabetes |
| title_sort | clonal diversity of the b cell receptor repertoire in patients with coronary in stent restenosis and type 2 diabetes |
| topic | b cell receptor repertoire repertoire sequencing in-stent restenosis type 2 diabetes mellitus |
| url | https://doi.org/10.1515/biol-2021-0091 |
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