Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study
Abstract Background: Acrylamide (AA) is a carcinogenic compound that causes severe reproductive impairments and represents a high environmental risk factor. Thymoquinone (TQ) has a unique antioxidant activity and has been widely used as a protective agent against various types of toxicity. Objective...
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2023-01-01
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| Series: | International Journal of Reproductive BioMedicine |
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| Online Access: | https://doi.org/10.18502/ijrm.v21i1.12668 |
| _version_ | 1797924694968500224 |
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| author | Maryam AL-ghamdi Etimad Huwait Nagwa Elsawi Soad Shaker Ali Ahmed Sayed |
| author_facet | Maryam AL-ghamdi Etimad Huwait Nagwa Elsawi Soad Shaker Ali Ahmed Sayed |
| author_sort | Maryam AL-ghamdi |
| collection | DOAJ |
| description | Abstract Background: Acrylamide (AA) is a carcinogenic compound that causes severe reproductive impairments and represents a high environmental risk factor. Thymoquinone (TQ) has a unique antioxidant activity and has been widely used as a protective agent against various types of toxicity. Objective: To evaluate the protective effects of TQ against AA-induced reproductive toxicity in female rats. Materials and Methods: In this experimental study, 40 female albino rats (120-150 gr, 8-10 wk) were sorted into 4 groups, (n = 10/each), vehicle group (received a daily oral administration of 0.5 ml saline [9%]); AA group (received a daily oral administration with freshly prepared AA, 20 mg/kg body weight) for 21 days which is less than the lethal dose LD 50 of AA in rats (20 mg/kg body weight); AA+TQ group (received a daily oral administration of TQ, 10 mg/kg body weight) after AA intoxication for 21 days, and TQ group (received a daily oral administration of TQ only, 10 mg/kg body weight) for 21 consecutive days. Reproductive hormones, carcinogenic biomarkers, and oxidative stress markers were measured. The histological assessment showed the protective effect of TQ against AA-induced ovarian injury. Network pharmacology analysis and molecular docking approach were carried out to determine the binding affinity of TQ with cyclooxygenase 2. Results: TQ administration significantly enhanced the functional capacity of the ovary at hormones, oxidative biomarkers, and tumor markers at a significant level of p < 0.001. Besides, TQ protects the ovary of AA-treated rats from the severe degeneration effect. Conclusion: TQ showed a promising protective effect against AA-induced reproductive toxicity in female rats. |
| first_indexed | 2024-04-10T15:05:02Z |
| format | Article |
| id | doaj.art-229de24c44834148889a91a82eb28d18 |
| institution | Directory Open Access Journal |
| issn | 2476-4108 2476-3772 |
| language | English |
| last_indexed | 2024-04-10T15:05:02Z |
| publishDate | 2023-01-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-229de24c44834148889a91a82eb28d182023-02-15T05:02:33ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722023-01-01211617010.18502/ijrm.v21i1.12668ijrm.v21i1.12668Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental studyMaryam AL-ghamdi0Etimad Huwait1Nagwa Elsawi2Soad Shaker Ali3Ahmed Sayed4 Department of Biochemistry, Faculty of Science, King AbdulAziz University, Jeddah, Saudi Arabia. Department of Biochemistry, Faculty of Science, King AbdulAziz University, Jeddah, Saudi Arabia. Department of Chemistry, Lab Biochemistry, Faculty of Science, Sohag University, Sohag, Egypt. Faculty of Medicine, Merit University, Sohag, Egypt. Biochemistry Laboratory, Faculty of Science, Chemistry Department, Assiut University, Assiut, Egypt.Abstract Background: Acrylamide (AA) is a carcinogenic compound that causes severe reproductive impairments and represents a high environmental risk factor. Thymoquinone (TQ) has a unique antioxidant activity and has been widely used as a protective agent against various types of toxicity. Objective: To evaluate the protective effects of TQ against AA-induced reproductive toxicity in female rats. Materials and Methods: In this experimental study, 40 female albino rats (120-150 gr, 8-10 wk) were sorted into 4 groups, (n = 10/each), vehicle group (received a daily oral administration of 0.5 ml saline [9%]); AA group (received a daily oral administration with freshly prepared AA, 20 mg/kg body weight) for 21 days which is less than the lethal dose LD 50 of AA in rats (20 mg/kg body weight); AA+TQ group (received a daily oral administration of TQ, 10 mg/kg body weight) after AA intoxication for 21 days, and TQ group (received a daily oral administration of TQ only, 10 mg/kg body weight) for 21 consecutive days. Reproductive hormones, carcinogenic biomarkers, and oxidative stress markers were measured. The histological assessment showed the protective effect of TQ against AA-induced ovarian injury. Network pharmacology analysis and molecular docking approach were carried out to determine the binding affinity of TQ with cyclooxygenase 2. Results: TQ administration significantly enhanced the functional capacity of the ovary at hormones, oxidative biomarkers, and tumor markers at a significant level of p < 0.001. Besides, TQ protects the ovary of AA-treated rats from the severe degeneration effect. Conclusion: TQ showed a promising protective effect against AA-induced reproductive toxicity in female rats.https://doi.org/10.18502/ijrm.v21i1.12668acrylamide, thymoquinone, rats, oxidative stress, cyclooxygenase 2, inflammation. |
| spellingShingle | Maryam AL-ghamdi Etimad Huwait Nagwa Elsawi Soad Shaker Ali Ahmed Sayed Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study International Journal of Reproductive BioMedicine acrylamide, thymoquinone, rats, oxidative stress, cyclooxygenase 2, inflammation. |
| title | Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study |
| title_full | Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study |
| title_fullStr | Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study |
| title_full_unstemmed | Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study |
| title_short | Thymoquinone ameliorates acrylamide-induced reproductive toxicity in female rats: An experimental study |
| title_sort | thymoquinone ameliorates acrylamide induced reproductive toxicity in female rats an experimental study |
| topic | acrylamide, thymoquinone, rats, oxidative stress, cyclooxygenase 2, inflammation. |
| url | https://doi.org/10.18502/ijrm.v21i1.12668 |
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