Microarray analyses of the infant gut microbiota

To detect the cause of germ free infant gut become colonization after birth and develops though the pregnancy for mother, 4 days, 10 days. 4 months, 1 year to 2 years is important, which has strong relationship with health and disease infant. This research was carried out at the experimental laborat...

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Main Authors: Lubna Ahmed Abdulkarim, Chra Saed Abdulla, Farhad Tahir Mahmod
Format: Article
Language:English
Published: Salahaddin University-Erbil 2019-06-01
Series:Zanco Journal of Pure and Applied Sciences
Subjects:
Online Access:https://zancojournals.su.edu.krd/index.php/JPAS/article/view/2542
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author Lubna Ahmed Abdulkarim
Chra Saed Abdulla
Farhad Tahir Mahmod
author_facet Lubna Ahmed Abdulkarim
Chra Saed Abdulla
Farhad Tahir Mahmod
author_sort Lubna Ahmed Abdulkarim
collection DOAJ
description To detect the cause of germ free infant gut become colonization after birth and develops though the pregnancy for mother, 4 days, 10 days. 4 months, 1 year to 2 years is important, which has strong relationship with health and disease infant. This research was carried out at the experimental laboratory of molecular microbiology department at Norwegian University Of Life Science, College of of NMBU, Ås la Norge / Norway, during Jan 2015 to get more information about the development of microbiota in infant. The pattern of colonizers is different at the different age. The purify faecal sample DNA perform universal amplification of the 16Sr RNA to the determination of gut microbiota by the GA-map infant array and 454 pyrosequencing. The results showed implicating the Bifidobacteria and Firmicutes that present in great amount during the development of the infant gut microbiota with reach to peak at the age of several weeks. A temporal trend in the microbiota composition was also detected which indicates the usefulness of this method for determining the temporal dynamics of the infant gut development.
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spelling doaj.art-229e9177e2e74e3db22a6ec1563f54d02022-12-21T23:35:19ZengSalahaddin University-ErbilZanco Journal of Pure and Applied Sciences2218-02302412-39862019-06-01313526310.21271/ZJPAS.31.3.8Microarray analyses of the infant gut microbiotaLubna Ahmed Abdulkarim0Chra Saed Abdulla1Farhad Tahir Mahmod2Department Environmental Sciences, College of Science, Salahaddin University-Erbil, Kurdistan Region, IraqDepartment of Animal source , College of Agriculture, Salahaddin University- Erbil, Kurdistan Region, IraqFood Safety Authority, Norway.To detect the cause of germ free infant gut become colonization after birth and develops though the pregnancy for mother, 4 days, 10 days. 4 months, 1 year to 2 years is important, which has strong relationship with health and disease infant. This research was carried out at the experimental laboratory of molecular microbiology department at Norwegian University Of Life Science, College of of NMBU, Ås la Norge / Norway, during Jan 2015 to get more information about the development of microbiota in infant. The pattern of colonizers is different at the different age. The purify faecal sample DNA perform universal amplification of the 16Sr RNA to the determination of gut microbiota by the GA-map infant array and 454 pyrosequencing. The results showed implicating the Bifidobacteria and Firmicutes that present in great amount during the development of the infant gut microbiota with reach to peak at the age of several weeks. A temporal trend in the microbiota composition was also detected which indicates the usefulness of this method for determining the temporal dynamics of the infant gut development.https://zancojournals.su.edu.krd/index.php/JPAS/article/view/2542MicrobiotaGutFecalInfant
spellingShingle Lubna Ahmed Abdulkarim
Chra Saed Abdulla
Farhad Tahir Mahmod
Microarray analyses of the infant gut microbiota
Zanco Journal of Pure and Applied Sciences
Microbiota
Gut
Fecal
Infant
title Microarray analyses of the infant gut microbiota
title_full Microarray analyses of the infant gut microbiota
title_fullStr Microarray analyses of the infant gut microbiota
title_full_unstemmed Microarray analyses of the infant gut microbiota
title_short Microarray analyses of the infant gut microbiota
title_sort microarray analyses of the infant gut microbiota
topic Microbiota
Gut
Fecal
Infant
url https://zancojournals.su.edu.krd/index.php/JPAS/article/view/2542
work_keys_str_mv AT lubnaahmedabdulkarim microarrayanalysesoftheinfantgutmicrobiota
AT chrasaedabdulla microarrayanalysesoftheinfantgutmicrobiota
AT farhadtahirmahmod microarrayanalysesoftheinfantgutmicrobiota