Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1
Synaptic α‐amino‐3‐hydroxyl‐5‐methyl‐4‐isoxazole‐propionate (AMPA) receptors are essential mediators of neurotransmission in the central nervous system. Shisa9/cysteine‐knot AMPAR modulating protein 44 (CKAMP44) is a transmembrane protein recently found to be present in AMPA receptor‐associated prot...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-09-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12261 |
| _version_ | 1817971010468577280 |
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| author | Stella‐Amrei Kunde Nils Rademacher Hanna Zieger Sarah A. Shoichet |
| author_facet | Stella‐Amrei Kunde Nils Rademacher Hanna Zieger Sarah A. Shoichet |
| author_sort | Stella‐Amrei Kunde |
| collection | DOAJ |
| description | Synaptic α‐amino‐3‐hydroxyl‐5‐methyl‐4‐isoxazole‐propionate (AMPA) receptors are essential mediators of neurotransmission in the central nervous system. Shisa9/cysteine‐knot AMPAR modulating protein 44 (CKAMP44) is a transmembrane protein recently found to be present in AMPA receptor‐associated protein complexes. Here, we show that the cytosolic tail of Shisa9/CKAMP44 interacts with multiple scaffold proteins that are important for regulating synaptic plasticity in central neurons. We focussed on the interaction with the scaffold protein PICK1, which facilitates the formation of a tripartite complex with the protein kinase C (PKC) and thereby regulates phosphorylation of Shisa9/CKAMP44 C‐terminal residues. This work has implications for our understanding of how PICK1 modulates AMPAR‐mediated transmission and plasticity and also highlights a novel function of PKC. |
| first_indexed | 2024-04-13T20:41:23Z |
| format | Article |
| id | doaj.art-229fbf70d9be4ae89a3629d250eb6e70 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-13T20:41:23Z |
| publishDate | 2017-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-229fbf70d9be4ae89a3629d250eb6e702022-12-22T02:30:51ZengWileyFEBS Open Bio2211-54632017-09-01791234124510.1002/2211-5463.12261Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1Stella‐Amrei Kunde0Nils Rademacher1Hanna Zieger2Sarah A. Shoichet3Neuroscience Research Center/Institute of Biochemistry Charité – Universitätsmedizin Berlin GermanyNeuroscience Research Center/Institute of Biochemistry Charité – Universitätsmedizin Berlin GermanyNeuroscience Research Center/Institute of Biochemistry Charité – Universitätsmedizin Berlin GermanyNeuroscience Research Center/Institute of Biochemistry Charité – Universitätsmedizin Berlin GermanySynaptic α‐amino‐3‐hydroxyl‐5‐methyl‐4‐isoxazole‐propionate (AMPA) receptors are essential mediators of neurotransmission in the central nervous system. Shisa9/cysteine‐knot AMPAR modulating protein 44 (CKAMP44) is a transmembrane protein recently found to be present in AMPA receptor‐associated protein complexes. Here, we show that the cytosolic tail of Shisa9/CKAMP44 interacts with multiple scaffold proteins that are important for regulating synaptic plasticity in central neurons. We focussed on the interaction with the scaffold protein PICK1, which facilitates the formation of a tripartite complex with the protein kinase C (PKC) and thereby regulates phosphorylation of Shisa9/CKAMP44 C‐terminal residues. This work has implications for our understanding of how PICK1 modulates AMPAR‐mediated transmission and plasticity and also highlights a novel function of PKC.https://doi.org/10.1002/2211-5463.12261AMPAR auxiliary proteinmolecular neurobiologyphosphorylationPKCpostsynaptic proteins |
| spellingShingle | Stella‐Amrei Kunde Nils Rademacher Hanna Zieger Sarah A. Shoichet Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1 FEBS Open Bio AMPAR auxiliary protein molecular neurobiology phosphorylation PKC postsynaptic proteins |
| title | Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1 |
| title_full | Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1 |
| title_fullStr | Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1 |
| title_full_unstemmed | Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1 |
| title_short | Protein kinase C regulates AMPA receptor auxiliary protein Shisa9/CKAMP44 through interactions with neuronal scaffold PICK1 |
| title_sort | protein kinase c regulates ampa receptor auxiliary protein shisa9 ckamp44 through interactions with neuronal scaffold pick1 |
| topic | AMPAR auxiliary protein molecular neurobiology phosphorylation PKC postsynaptic proteins |
| url | https://doi.org/10.1002/2211-5463.12261 |
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