Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity
Abstract To achieve better antitumour efficacy, it is urgent to improve anticancer drug delivery efficiency in targeting cancer cells. In this work, chitosan-functionalized graphene oxide (ChrGO) nanosheets were fabricated via microwave-assisted reduction, which were employed to the intracellular de...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-04-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s11671-021-03525-y |
| _version_ | 1797698945877540864 |
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| author | Mengjun Shu Feng Gao Min Zeng Chulang Yu Xue Wang Renhua Huang Jianhua Yang Yanjie Su Nantao Hu Zhihua Zhou Ke Liu Zhi Yang Hongtao Tan Lin Xu |
| author_facet | Mengjun Shu Feng Gao Min Zeng Chulang Yu Xue Wang Renhua Huang Jianhua Yang Yanjie Su Nantao Hu Zhihua Zhou Ke Liu Zhi Yang Hongtao Tan Lin Xu |
| author_sort | Mengjun Shu |
| collection | DOAJ |
| description | Abstract To achieve better antitumour efficacy, it is urgent to improve anticancer drug delivery efficiency in targeting cancer cells. In this work, chitosan-functionalized graphene oxide (ChrGO) nanosheets were fabricated via microwave-assisted reduction, which were employed to the intracellular delivery nanosystem for anticancer drug agent in breast cancer cells. Drug loading and release research indicated that adriamycin can be efficiently loaded on and released from the ChrGO nanosheets. Less drug release during delivery and better biocompatibility of ChrGO/adriamycin significantly improve its safety and therapeutic efficacy in HER2-overexpressing BT-474 cells. Furthermore, ChrGO/adriamycin in combination with trastuzumab exhibited synergistic antitumour activity in BT-474 cells, which demonstrated superior therapeutic efficacy compared with each drug alone. Cells treated with trastuzumab (5 μg/mL) or equivalent ChrGO/adriamycin (5 μg/mL) each elicited 54.5% and 59.5% cell death, respectively, while the combination treatment with trastuzumab and ChrGO/adriamycin resulted in a dramatic 88.5% cell death. The dual-targeted therapy displayed higher apoptosis, indicating superior therapeutic efficacy due to the presence of different mechanisms of action. The combined treatment of ChrGO/adriamycin and trastuzumab in BT-474 cells induced cell cycle arrest and apoptosis, which ultimately led to the death of augmented cancer cells. This work has provided a facile microwave-assisted fabrication of ChrGO as a controlled and targeted intracellular drug delivery nanosystem, which is expected to be a novel promising therapy for treating HER2-overexpressing breast cancer cells. |
| first_indexed | 2024-03-12T04:01:21Z |
| format | Article |
| id | doaj.art-22a734935eda4c5c8ed41aff24a7ed3f |
| institution | Directory Open Access Journal |
| issn | 1556-276X |
| language | English |
| last_indexed | 2024-03-12T04:01:21Z |
| publishDate | 2021-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-22a734935eda4c5c8ed41aff24a7ed3f2023-09-03T11:34:05ZengSpringerOpenNanoscale Research Letters1556-276X2021-04-0116111210.1186/s11671-021-03525-yMicrowave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour ActivityMengjun Shu0Feng Gao1Min Zeng2Chulang Yu3Xue Wang4Renhua Huang5Jianhua Yang6Yanjie Su7Nantao Hu8Zhihua Zhou9Ke Liu10Zhi Yang11Hongtao Tan12Lin Xu13Key Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityKey Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityKey Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityState Key Laboratory for Managing Biotic and Chemical Threats To the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MOA and Zhejiang Province, Institute of Plant Virology, Ningbo UniversityDepartment of Dermatology, Shanghai Ninth People’s Hospital, Affiliated To Shanghai Jiao Tong University School of Medicine, Center for Specialty Strategy Research of Shanghai Jiao Tong University China Hospital Development InstituteDepartment of Radiation, Renji Hospital, School of Medicine, Shanghai Jiao Tong UniversityKey Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityKey Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityKey Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityKey Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityDepartment of Dermatology, Shanghai Ninth People’s Hospital, Affiliated To Shanghai Jiao Tong University School of Medicine, Center for Specialty Strategy Research of Shanghai Jiao Tong University China Hospital Development InstituteKey Laboratory of Thin Film and Microfabrication (Ministry of Education), Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong UniversityKey Laboratory of Hepatosplenic Surgery (Ministry of Education), Department of General Surgery, The First Affiliated Hospital of Harbin Medical UniversityDepartment of Ophthalmogy, Shanghai General Hospital (Shanghai First People’s Hospital), School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai Eye Disease Prevention and Treatment Center/Shanghai Eye Hospital, National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science and PhotomedicineAbstract To achieve better antitumour efficacy, it is urgent to improve anticancer drug delivery efficiency in targeting cancer cells. In this work, chitosan-functionalized graphene oxide (ChrGO) nanosheets were fabricated via microwave-assisted reduction, which were employed to the intracellular delivery nanosystem for anticancer drug agent in breast cancer cells. Drug loading and release research indicated that adriamycin can be efficiently loaded on and released from the ChrGO nanosheets. Less drug release during delivery and better biocompatibility of ChrGO/adriamycin significantly improve its safety and therapeutic efficacy in HER2-overexpressing BT-474 cells. Furthermore, ChrGO/adriamycin in combination with trastuzumab exhibited synergistic antitumour activity in BT-474 cells, which demonstrated superior therapeutic efficacy compared with each drug alone. Cells treated with trastuzumab (5 μg/mL) or equivalent ChrGO/adriamycin (5 μg/mL) each elicited 54.5% and 59.5% cell death, respectively, while the combination treatment with trastuzumab and ChrGO/adriamycin resulted in a dramatic 88.5% cell death. The dual-targeted therapy displayed higher apoptosis, indicating superior therapeutic efficacy due to the presence of different mechanisms of action. The combined treatment of ChrGO/adriamycin and trastuzumab in BT-474 cells induced cell cycle arrest and apoptosis, which ultimately led to the death of augmented cancer cells. This work has provided a facile microwave-assisted fabrication of ChrGO as a controlled and targeted intracellular drug delivery nanosystem, which is expected to be a novel promising therapy for treating HER2-overexpressing breast cancer cells.https://doi.org/10.1186/s11671-021-03525-yGraphene oxideDrug deliveryAdriamycinMicrowave-assisted reductionBreast cancerHER2 |
| spellingShingle | Mengjun Shu Feng Gao Min Zeng Chulang Yu Xue Wang Renhua Huang Jianhua Yang Yanjie Su Nantao Hu Zhihua Zhou Ke Liu Zhi Yang Hongtao Tan Lin Xu Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity Nanoscale Research Letters Graphene oxide Drug delivery Adriamycin Microwave-assisted reduction Breast cancer HER2 |
| title | Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity |
| title_full | Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity |
| title_fullStr | Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity |
| title_full_unstemmed | Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity |
| title_short | Microwave-Assisted Chitosan-Functionalized Graphene Oxide as Controlled Intracellular Drug Delivery Nanosystem for Synergistic Antitumour Activity |
| title_sort | microwave assisted chitosan functionalized graphene oxide as controlled intracellular drug delivery nanosystem for synergistic antitumour activity |
| topic | Graphene oxide Drug delivery Adriamycin Microwave-assisted reduction Breast cancer HER2 |
| url | https://doi.org/10.1186/s11671-021-03525-y |
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