Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis
Fluoxetine is an antidepressant, a selective serotonin reuptake inhibitor (SSRI) used primarily in the treatment of major depression, panic disorder and obsessive compulsive disorder. Chiral separation of racemic fluoxetine is necessary due to its enantioselective metabolism. In order to develop a s...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016416300998 |
| _version_ | 1811324599684562944 |
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| author | Melania Cârcu-Dobrin Monica Budău Gabriel Hancu Laszlo Gagyi Aura Rusu Hajnal Kelemen |
| author_facet | Melania Cârcu-Dobrin Monica Budău Gabriel Hancu Laszlo Gagyi Aura Rusu Hajnal Kelemen |
| author_sort | Melania Cârcu-Dobrin |
| collection | DOAJ |
| description | Fluoxetine is an antidepressant, a selective serotonin reuptake inhibitor (SSRI) used primarily in the treatment of major depression, panic disorder and obsessive compulsive disorder. Chiral separation of racemic fluoxetine is necessary due to its enantioselective metabolism. In order to develop a suitable method for chiral separation of fluoxetine, cyclodextrin (CD) modified capillary electrophoresis (CE) was employed. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector. As a result of this process, heptakis(2,3,6-tri-O-methyl)-β-CD (TRIMEB) was selected for enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors are varied at the same time to optimize the separation method. The optimized method (50 mM phosphate buffer, pH = 5.0, 10 mM TRIMEB, 15 °C, + 20 kV, 50 mbar/1 s, detection at 230 nm) was successful for baseline separation of fluoxetine enantiomers within 5 min. Our method was validated according to ICH guidelines and proved to be sensitive, linear, accurate and precise for the chiral separation of fluoxetine. |
| first_indexed | 2024-04-13T14:18:21Z |
| format | Article |
| id | doaj.art-22a81ceb7e91411791f240a311162189 |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-13T14:18:21Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-22a81ceb7e91411791f240a3111621892022-12-22T02:43:35ZengElsevierSaudi Pharmaceutical Journal1319-01642017-03-0125339740310.1016/j.jsps.2016.09.007Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresisMelania Cârcu-Dobrin0Monica Budău1Gabriel Hancu2Laszlo Gagyi3Aura Rusu4Hajnal Kelemen5Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş, RomaniaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş, RomaniaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş, RomaniaSC VimSpectrum SRL, Tîrgu Mureş, RomaniaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş, RomaniaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy of Tîrgu Mureş, Tîrgu Mureş, RomaniaFluoxetine is an antidepressant, a selective serotonin reuptake inhibitor (SSRI) used primarily in the treatment of major depression, panic disorder and obsessive compulsive disorder. Chiral separation of racemic fluoxetine is necessary due to its enantioselective metabolism. In order to develop a suitable method for chiral separation of fluoxetine, cyclodextrin (CD) modified capillary electrophoresis (CE) was employed. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector. As a result of this process, heptakis(2,3,6-tri-O-methyl)-β-CD (TRIMEB) was selected for enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors are varied at the same time to optimize the separation method. The optimized method (50 mM phosphate buffer, pH = 5.0, 10 mM TRIMEB, 15 °C, + 20 kV, 50 mbar/1 s, detection at 230 nm) was successful for baseline separation of fluoxetine enantiomers within 5 min. Our method was validated according to ICH guidelines and proved to be sensitive, linear, accurate and precise for the chiral separation of fluoxetine.http://www.sciencedirect.com/science/article/pii/S1319016416300998FluoxetineSelective serotonin reuptake inhibitorCapillary electrophoresisChiral separationCyclodextrines |
| spellingShingle | Melania Cârcu-Dobrin Monica Budău Gabriel Hancu Laszlo Gagyi Aura Rusu Hajnal Kelemen Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis Saudi Pharmaceutical Journal Fluoxetine Selective serotonin reuptake inhibitor Capillary electrophoresis Chiral separation Cyclodextrines |
| title | Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis |
| title_full | Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis |
| title_fullStr | Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis |
| title_full_unstemmed | Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis |
| title_short | Enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis |
| title_sort | enantioselective analysis of fluoxetine in pharmaceutical formulations by capillary zone electrophoresis |
| topic | Fluoxetine Selective serotonin reuptake inhibitor Capillary electrophoresis Chiral separation Cyclodextrines |
| url | http://www.sciencedirect.com/science/article/pii/S1319016416300998 |
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