Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated Immortalization
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors in the digestive tract and originate from the interstitial cells of Cajal (ICC), which is the pacemaker for peristaltic movement in the gastrointestinal tract. Existing GIST cell lines are widely used as cell models for i...
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| Format: | Article |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13640 |
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| author | Xiangchen Hu Peng Su Bo Liu Jingwei Guo Zitong Wang Cai He Zhe Wang Youwei Kou |
| author_facet | Xiangchen Hu Peng Su Bo Liu Jingwei Guo Zitong Wang Cai He Zhe Wang Youwei Kou |
| author_sort | Xiangchen Hu |
| collection | DOAJ |
| description | Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors in the digestive tract and originate from the interstitial cells of Cajal (ICC), which is the pacemaker for peristaltic movement in the gastrointestinal tract. Existing GIST cell lines are widely used as cell models for in vitro experimental studies because the mutation sites are known. However, the immortalization methods of these cell lines are unknown, and no Chinese patient-derived GIST cell lines have been documented. Here, we transfected simian virus 40 large T antigen (SV40LT) into primary GIST cells to establish an immortalized human GIST cell line (ImGIST) for the first time. The ImGIST cells had neuronal cell-like irregular radioactive growth and retained the fusion growth characteristics of GIST cells. They stably expressed signature proteins, maintained the biological and genomic characteristics of normal primary GIST cells, and responded well to imatinib, suggesting that ImGIST could be a potential in vitro model for research in GIST to explore the molecular pathogenesis, drug resistance mechanisms, and the development of new adjuvant therapeutic options. |
| first_indexed | 2024-03-10T23:20:12Z |
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| id | doaj.art-22af5ea289d94f3e849f1bd0e0aca79c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:20:12Z |
| publishDate | 2023-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-22af5ea289d94f3e849f1bd0e0aca79c2023-11-19T08:20:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124171364010.3390/ijms241713640Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated ImmortalizationXiangchen Hu0Peng Su1Bo Liu2Jingwei Guo3Zitong Wang4Cai He5Zhe Wang6Youwei Kou7Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaMedical Research Center, Shengjing Hospital of China Medical University, Shenyang 117005, ChinaDepartment of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaDepartment of Pathology, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaDepartment of Pathology, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaDepartment of Pathology, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaDepartment of Pathology, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaDepartment of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, ChinaGastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors in the digestive tract and originate from the interstitial cells of Cajal (ICC), which is the pacemaker for peristaltic movement in the gastrointestinal tract. Existing GIST cell lines are widely used as cell models for in vitro experimental studies because the mutation sites are known. However, the immortalization methods of these cell lines are unknown, and no Chinese patient-derived GIST cell lines have been documented. Here, we transfected simian virus 40 large T antigen (SV40LT) into primary GIST cells to establish an immortalized human GIST cell line (ImGIST) for the first time. The ImGIST cells had neuronal cell-like irregular radioactive growth and retained the fusion growth characteristics of GIST cells. They stably expressed signature proteins, maintained the biological and genomic characteristics of normal primary GIST cells, and responded well to imatinib, suggesting that ImGIST could be a potential in vitro model for research in GIST to explore the molecular pathogenesis, drug resistance mechanisms, and the development of new adjuvant therapeutic options.https://www.mdpi.com/1422-0067/24/17/13640gastrointestinal stromal tumorimmortalizationSV40LTcell linesimatinib |
| spellingShingle | Xiangchen Hu Peng Su Bo Liu Jingwei Guo Zitong Wang Cai He Zhe Wang Youwei Kou Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated Immortalization International Journal of Molecular Sciences gastrointestinal stromal tumor immortalization SV40LT cell lines imatinib |
| title | Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated Immortalization |
| title_full | Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated Immortalization |
| title_fullStr | Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated Immortalization |
| title_full_unstemmed | Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated Immortalization |
| title_short | Characterization of a Human Gastrointestinal Stromal Tumor Cell Line Established by SV40LT-Mediated Immortalization |
| title_sort | characterization of a human gastrointestinal stromal tumor cell line established by sv40lt mediated immortalization |
| topic | gastrointestinal stromal tumor immortalization SV40LT cell lines imatinib |
| url | https://www.mdpi.com/1422-0067/24/17/13640 |
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