Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aorta
BackgroundThe intravenous administration of indigo carmine has been reported to produce transiently increased blood pressure in patients. The goal of this in vitro study was to examine the effect of indigo carmine on phenylephrine-induced contractions in an isolated rat aorta and to determine the as...
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| Format: | Article |
| Language: | English |
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Korean Society of Anesthesiologists
2011-07-01
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| Series: | Korean Journal of Anesthesiology |
| Subjects: | |
| Online Access: | http://ekja.org/upload/pdf/kjae-61-55.pdf |
| _version_ | 1817981765538545664 |
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| author | Yun Suk Choi Seong-Ho Ok Seung Min Lee Sang-Seung Park Yu Mi Ha Ki Churl Chang Hye Jung Kim Il-Woo Shin Ju-Tae Sohn |
| author_facet | Yun Suk Choi Seong-Ho Ok Seung Min Lee Sang-Seung Park Yu Mi Ha Ki Churl Chang Hye Jung Kim Il-Woo Shin Ju-Tae Sohn |
| author_sort | Yun Suk Choi |
| collection | DOAJ |
| description | BackgroundThe intravenous administration of indigo carmine has been reported to produce transiently increased blood pressure in patients. The goal of this in vitro study was to examine the effect of indigo carmine on phenylephrine-induced contractions in an isolated rat aorta and to determine the associated cellular mechanism with particular focus on the endothelium-derived vasodilators.MethodsThe concentration-response curves for phenylephrine were generated in the presence or absence of indigo carmine. Phenylephrine concentration-response curves were generated for the endothelium-intact rings pretreated independently with a nitric oxide synthase inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME), a cyclooxygenase inhibitor, indomethacin, and a low-molecular-weight superoxide anion scavenger, tiron, in the presence or absence of indigo carmine. The fluorescence of oxidized dichlorofluorescein was measured in rat aortic vascular smooth muscle cells cultured in the control, indigo carmine alone and tiron plus indigo carmine.ResultsIndigo carmine (10-5 M) increased the phenylephrine-induced maximum contraction in the endothelium-intact rings with or without indomethacin, whereas indigo carmine produced a slight leftward shift in the phenylephrine concentration-response curves in the endothelium-denuded rings and L-NAME-pretreated endothelium-intact rings. In the endothelium-intact rings pretreated with tiron (10-2 M), indigo carmine did not alter phenylephrine concentration-response curves significantly. Indigo carmine (10-5 M) increased the fluorescence of oxidized dichlorofluorescein in the vascular smooth muscle cells, whereas tiron abolished the indigo carmine-induced increase in oxidized dichlorofluorescein fluorescence.ConclusionsIndigo carmine increases the phenylephrine-induced contraction mainly through an endothelium-dependent mechanism involving the inactivation of nitric oxide caused by the increased production of reactive oxygen species. |
| first_indexed | 2024-04-13T23:10:28Z |
| format | Article |
| id | doaj.art-22b320659c5345689981fc470a97f57c |
| institution | Directory Open Access Journal |
| issn | 2005-6419 2005-7563 |
| language | English |
| last_indexed | 2024-04-13T23:10:28Z |
| publishDate | 2011-07-01 |
| publisher | Korean Society of Anesthesiologists |
| record_format | Article |
| series | Korean Journal of Anesthesiology |
| spelling | doaj.art-22b320659c5345689981fc470a97f57c2022-12-22T02:25:34ZengKorean Society of AnesthesiologistsKorean Journal of Anesthesiology2005-64192005-75632011-07-01611556210.4097/kjae.2011.61.1.557253Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aortaYun Suk Choi0Seong-Ho Ok1Seung Min Lee2Sang-Seung Park3Yu Mi Ha4Ki Churl Chang5Hye Jung Kim6Il-Woo Shin7Ju-Tae Sohn8Department of Anesthesiology and Pain Medicine, Jeju National University School of Medicine, Jeju, Korea.Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.Department of Pharmacology, Gyeongsang National University School of Medicine, Jinju, Korea.Department of Pharmacology, Gyeongsang National University School of Medicine, Jinju, Korea.Department of Pharmacology, Gyeongsang National University School of Medicine, Jinju, Korea.Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.Department of Anesthesiology and Pain Medicine, Gyeongsang National University School of Medicine, Jinju, Korea.BackgroundThe intravenous administration of indigo carmine has been reported to produce transiently increased blood pressure in patients. The goal of this in vitro study was to examine the effect of indigo carmine on phenylephrine-induced contractions in an isolated rat aorta and to determine the associated cellular mechanism with particular focus on the endothelium-derived vasodilators.MethodsThe concentration-response curves for phenylephrine were generated in the presence or absence of indigo carmine. Phenylephrine concentration-response curves were generated for the endothelium-intact rings pretreated independently with a nitric oxide synthase inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME), a cyclooxygenase inhibitor, indomethacin, and a low-molecular-weight superoxide anion scavenger, tiron, in the presence or absence of indigo carmine. The fluorescence of oxidized dichlorofluorescein was measured in rat aortic vascular smooth muscle cells cultured in the control, indigo carmine alone and tiron plus indigo carmine.ResultsIndigo carmine (10-5 M) increased the phenylephrine-induced maximum contraction in the endothelium-intact rings with or without indomethacin, whereas indigo carmine produced a slight leftward shift in the phenylephrine concentration-response curves in the endothelium-denuded rings and L-NAME-pretreated endothelium-intact rings. In the endothelium-intact rings pretreated with tiron (10-2 M), indigo carmine did not alter phenylephrine concentration-response curves significantly. Indigo carmine (10-5 M) increased the fluorescence of oxidized dichlorofluorescein in the vascular smooth muscle cells, whereas tiron abolished the indigo carmine-induced increase in oxidized dichlorofluorescein fluorescence.ConclusionsIndigo carmine increases the phenylephrine-induced contraction mainly through an endothelium-dependent mechanism involving the inactivation of nitric oxide caused by the increased production of reactive oxygen species.http://ekja.org/upload/pdf/kjae-61-55.pdfindigo carminenitric oxidephenylephrinereactive oxygen species |
| spellingShingle | Yun Suk Choi Seong-Ho Ok Seung Min Lee Sang-Seung Park Yu Mi Ha Ki Churl Chang Hye Jung Kim Il-Woo Shin Ju-Tae Sohn Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aorta Korean Journal of Anesthesiology indigo carmine nitric oxide phenylephrine reactive oxygen species |
| title | Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aorta |
| title_full | Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aorta |
| title_fullStr | Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aorta |
| title_full_unstemmed | Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aorta |
| title_short | Indigo carmine enhances phenylephrine-induced contractions in an isolated rat aorta |
| title_sort | indigo carmine enhances phenylephrine induced contractions in an isolated rat aorta |
| topic | indigo carmine nitric oxide phenylephrine reactive oxygen species |
| url | http://ekja.org/upload/pdf/kjae-61-55.pdf |
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