Microbial contamination in dental equipment and disinfection potential of different antimicrobial agents

ABSTRACT Objective: To analyze the dental equipment microbial contamination and to test different disinfectants, collaborating with the protocols control of cross infection in dental care. Methods: Samples were collected from dental equipment (syringes; auxiliary table; reflector), cultured in Pet...

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Main Authors: Stephanie Cezar de Mello TONELLO, Mateus José DUTRA, Gabriela PIZZOLATTO, Letícia de Abreu GIACOMINI, Daniela Jorge CORRALO
Format: Article
Language:English
Published: Faculdade São Leopoldo Mandic 2022-05-01
Series:RGO: Revista Gaúcha de Odontologia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372022000100307&tlng=en
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author Stephanie Cezar de Mello TONELLO
Mateus José DUTRA
Gabriela PIZZOLATTO
Letícia de Abreu GIACOMINI
Daniela Jorge CORRALO
author_facet Stephanie Cezar de Mello TONELLO
Mateus José DUTRA
Gabriela PIZZOLATTO
Letícia de Abreu GIACOMINI
Daniela Jorge CORRALO
author_sort Stephanie Cezar de Mello TONELLO
collection DOAJ
description ABSTRACT Objective: To analyze the dental equipment microbial contamination and to test different disinfectants, collaborating with the protocols control of cross infection in dental care. Methods: Samples were collected from dental equipment (syringes; auxiliary table; reflector), cultured in Petri plates with Brain Heart Agar (for bacteria) and Sabourad Agar (for fungi) culture medium. After collection of the initial samples, the surfaces were randomly divided and disinfected with the following products: ethanol 70% (A70); 5% chlorhexidine (CHX5) and, glucoprotamina 0.5% (GLP0,5). New sample collections were made from the same locations described above (final samples). Results: No disinfectant product tested was able to eliminate all microbial forms (bacteria and fungi) surfaces. For bacteria, the antimicrobial activity was higher with the ethanol 70%, followed by 5% chlorhexidine and glucoprotamina 0.5%. For fungi, the 5% chlorhexidine had the best effect, followed by ethanol 70% and glucoprotamina 0.5%. Conclusion: The study confirmed the contamination of surfaces of dental equipment and the importance of disinfection for infection control in the dental clinic. Through this study, no antimicrobial agent tested was 100% effective in eliminating microorganisms present in the dental clinic surfaces.
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spelling doaj.art-22b52099207b46d8aceee79454848ccb2023-06-28T23:15:55ZengFaculdade São Leopoldo MandicRGO: Revista Gaúcha de Odontologia1981-86372022-05-017010.1590/1981-86372022001620200046Microbial contamination in dental equipment and disinfection potential of different antimicrobial agentsStephanie Cezar de Mello TONELLOhttps://orcid.org/0000-0002-1751-1523Mateus José DUTRAhttps://orcid.org/0000-0002-8338-3857Gabriela PIZZOLATTOhttps://orcid.org/0000-0003-1105-2482Letícia de Abreu GIACOMINIhttps://orcid.org/0000-0003-3982-6048Daniela Jorge CORRALOhttps://orcid.org/0000-0003-3034-1730ABSTRACT Objective: To analyze the dental equipment microbial contamination and to test different disinfectants, collaborating with the protocols control of cross infection in dental care. Methods: Samples were collected from dental equipment (syringes; auxiliary table; reflector), cultured in Petri plates with Brain Heart Agar (for bacteria) and Sabourad Agar (for fungi) culture medium. After collection of the initial samples, the surfaces were randomly divided and disinfected with the following products: ethanol 70% (A70); 5% chlorhexidine (CHX5) and, glucoprotamina 0.5% (GLP0,5). New sample collections were made from the same locations described above (final samples). Results: No disinfectant product tested was able to eliminate all microbial forms (bacteria and fungi) surfaces. For bacteria, the antimicrobial activity was higher with the ethanol 70%, followed by 5% chlorhexidine and glucoprotamina 0.5%. For fungi, the 5% chlorhexidine had the best effect, followed by ethanol 70% and glucoprotamina 0.5%. Conclusion: The study confirmed the contamination of surfaces of dental equipment and the importance of disinfection for infection control in the dental clinic. Through this study, no antimicrobial agent tested was 100% effective in eliminating microorganisms present in the dental clinic surfaces.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372022000100307&tlng=enChlorhexidineContainment of BiohazardsDental clinicsDisinfectionEthanol
spellingShingle Stephanie Cezar de Mello TONELLO
Mateus José DUTRA
Gabriela PIZZOLATTO
Letícia de Abreu GIACOMINI
Daniela Jorge CORRALO
Microbial contamination in dental equipment and disinfection potential of different antimicrobial agents
RGO: Revista Gaúcha de Odontologia
Chlorhexidine
Containment of Biohazards
Dental clinics
Disinfection
Ethanol
title Microbial contamination in dental equipment and disinfection potential of different antimicrobial agents
title_full Microbial contamination in dental equipment and disinfection potential of different antimicrobial agents
title_fullStr Microbial contamination in dental equipment and disinfection potential of different antimicrobial agents
title_full_unstemmed Microbial contamination in dental equipment and disinfection potential of different antimicrobial agents
title_short Microbial contamination in dental equipment and disinfection potential of different antimicrobial agents
title_sort microbial contamination in dental equipment and disinfection potential of different antimicrobial agents
topic Chlorhexidine
Containment of Biohazards
Dental clinics
Disinfection
Ethanol
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-86372022000100307&tlng=en
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AT leticiadeabreugiacomini microbialcontaminationindentalequipmentanddisinfectionpotentialofdifferentantimicrobialagents
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