A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk
ABSTRACT: Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a Fe3O4@graphene modified electrode. Because of the excellent electrical conductivity and mechanical stability o...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
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| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030221011061 |
| _version_ | 1811228397197590528 |
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| author | Kaiwen Feng Ting Li Cuizhu Ye Xiaoyu Gao Xianglin Yue Shuangyan Ding Qiuling Dong Mingqi Yang Ganhui Huang Jinsheng Zhang |
| author_facet | Kaiwen Feng Ting Li Cuizhu Ye Xiaoyu Gao Xianglin Yue Shuangyan Ding Qiuling Dong Mingqi Yang Ganhui Huang Jinsheng Zhang |
| author_sort | Kaiwen Feng |
| collection | DOAJ |
| description | ABSTRACT: Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a Fe3O4@graphene modified electrode. Because of the excellent electrical conductivity and mechanical stability of graphene and the large specific surface area of Fe3O4, the Fe3O4@graphene nanocomposite exhibits an excellent electrical signal, which greatly increased the sensitivity of the immunosensor. Gold nanoparticles were deposited on Fe3O4@graphene nanocomposite by electrochemical technology for the immobilization of the antibody. Cyclic voltammetry was selected to electrochemically characterize the construction process of immunosensors. The microstructure and morphology of related nanocomposites were analyzed by scanning electron microscopy. Under optimized experimental conditions, a good linear relationship was achieved in the Salmonella concentration range of 2.4 × 102 to 2.4 × 107 cfu/mL, and the limit of detection of the immunosensor was 2.4 × 102 cfu/mL. Additionally, the constructed immunosensor exhibited acceptable selectivity, reproducibility, and stability and provides a new reference for detecting pathogenic bacteria in milk. |
| first_indexed | 2024-04-12T09:58:22Z |
| format | Article |
| id | doaj.art-22b584b6de1a4f94b2c95519d7cec069 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-04-12T09:58:22Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-22b584b6de1a4f94b2c95519d7cec0692022-12-22T03:37:38ZengElsevierJournal of Dairy Science0022-03022022-03-01105321082118A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milkKaiwen Feng0Ting Li1Cuizhu Ye2Xiaoyu Gao3Xianglin Yue4Shuangyan Ding5Qiuling Dong6Mingqi Yang7Ganhui Huang8Jinsheng Zhang9State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaCorresponding author; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, P. R. ChinaABSTRACT: Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a Fe3O4@graphene modified electrode. Because of the excellent electrical conductivity and mechanical stability of graphene and the large specific surface area of Fe3O4, the Fe3O4@graphene nanocomposite exhibits an excellent electrical signal, which greatly increased the sensitivity of the immunosensor. Gold nanoparticles were deposited on Fe3O4@graphene nanocomposite by electrochemical technology for the immobilization of the antibody. Cyclic voltammetry was selected to electrochemically characterize the construction process of immunosensors. The microstructure and morphology of related nanocomposites were analyzed by scanning electron microscopy. Under optimized experimental conditions, a good linear relationship was achieved in the Salmonella concentration range of 2.4 × 102 to 2.4 × 107 cfu/mL, and the limit of detection of the immunosensor was 2.4 × 102 cfu/mL. Additionally, the constructed immunosensor exhibited acceptable selectivity, reproducibility, and stability and provides a new reference for detecting pathogenic bacteria in milk.http://www.sciencedirect.com/science/article/pii/S0022030221011061SalmonellamilkimmunosensorFe3O4@graphene nanocompositegold nanoparticles |
| spellingShingle | Kaiwen Feng Ting Li Cuizhu Ye Xiaoyu Gao Xianglin Yue Shuangyan Ding Qiuling Dong Mingqi Yang Ganhui Huang Jinsheng Zhang A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk Journal of Dairy Science Salmonella milk immunosensor Fe3O4@graphene nanocomposite gold nanoparticles |
| title | A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk |
| title_full | A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk |
| title_fullStr | A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk |
| title_full_unstemmed | A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk |
| title_short | A novel electrochemical immunosensor based on Fe3O4@graphene nanocomposite modified glassy carbon electrode for rapid detection of Salmonella in milk |
| title_sort | novel electrochemical immunosensor based on fe3o4 graphene nanocomposite modified glassy carbon electrode for rapid detection of salmonella in milk |
| topic | Salmonella milk immunosensor Fe3O4@graphene nanocomposite gold nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S0022030221011061 |
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