Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virus
Background: Human papilloma virus (HPV) is one of the most important factors in cervical cancer. Viral sequences are integrated into the host cell genome. In mild cases the virus causes skin damages, in severe cases it leads to cancer. Like many other cancers, telomerase gene expression was increase...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2016-07-01
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| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5461&slc_lang=en&sid=1 |
| _version_ | 1811202690419523584 |
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| author | Maryam Akhtari Mahdi Kamali Gholam Reza Javadi Seyedeh Razieh Hashemi |
| author_facet | Maryam Akhtari Mahdi Kamali Gholam Reza Javadi Seyedeh Razieh Hashemi |
| author_sort | Maryam Akhtari |
| collection | DOAJ |
| description | Background: Human papilloma virus (HPV) is one of the most important factors in cervical cancer. Viral sequences are integrated into the host cell genome. In mild cases the virus causes skin damages, in severe cases it leads to cancer. Like many other cancers, telomerase gene expression was increased in cervical cancer. This enzyme is a reverse transcriptase that contains two common subunits: i) catalytic protein called human telomerase reverse transcriptase (hTERT) and, ii) RNA sequence called hTR. hTERT expression is hardly found in any somatic tissues. Detection of high telomerase activity in human cells, lead to tumor genesis. So hTERT can be used as a diagnostic tool in cancer detection.
Methods: This experimental study was carried out from May 2013 to April 2014 in Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences in Tehran, Iran. Caski and Hela cancer cell lines were used which contain HPV16 and HPV18 respectively. Cell lines were cultured and total RNA was extracted. Following normalization agent glyceraldehyde-3-phosphate dehydrogenase (GADPH), hTERT expression level was determining by real-time PCR method. For each sample, the expression level of hTERT and GAPDH were quantified as copy numbers (per reaction) using the standard curve. Finally, hTERT levels in Hela and Caski cell lines were compared quantitatively by t-test using GraphPad statistic software version 5 (San Diego, CA, USA).
Results: According to the charts real-time PCR, hTERT gene expression in Hela and Caski cancer cell lines is significantly different (t=0.0319).
Conclusion: All results confirm that hTERT expression levels in Hela and Caski cell lines are significantly different and the level of hTERT expression in the Caski cell line was slightly higher than that of Hela cell line. The significant difference between hTERT mRNA expression levels reported here could be used as a tumor marker for HPV16 and HPV18 in cervical cancer. |
| first_indexed | 2024-04-12T02:44:01Z |
| format | Article |
| id | doaj.art-22b9c8edff6a40708e323b1dfb23b3b0 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-04-12T02:44:01Z |
| publishDate | 2016-07-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-22b9c8edff6a40708e323b1dfb23b3b02022-12-22T03:51:15ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222016-07-01744260266Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virusMaryam Akhtari0Mahdi Kamali1Gholam Reza Javadi2Seyedeh Razieh Hashemi3 Department of Genetics, Science and Research Branch Islamic Azad University, Tehran, Iran. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Department of Genetics, Science and Research Branch Islamic Azad University, Tehran, Iran. Department of Obstetrics and Gynecology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. Background: Human papilloma virus (HPV) is one of the most important factors in cervical cancer. Viral sequences are integrated into the host cell genome. In mild cases the virus causes skin damages, in severe cases it leads to cancer. Like many other cancers, telomerase gene expression was increased in cervical cancer. This enzyme is a reverse transcriptase that contains two common subunits: i) catalytic protein called human telomerase reverse transcriptase (hTERT) and, ii) RNA sequence called hTR. hTERT expression is hardly found in any somatic tissues. Detection of high telomerase activity in human cells, lead to tumor genesis. So hTERT can be used as a diagnostic tool in cancer detection. Methods: This experimental study was carried out from May 2013 to April 2014 in Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences in Tehran, Iran. Caski and Hela cancer cell lines were used which contain HPV16 and HPV18 respectively. Cell lines were cultured and total RNA was extracted. Following normalization agent glyceraldehyde-3-phosphate dehydrogenase (GADPH), hTERT expression level was determining by real-time PCR method. For each sample, the expression level of hTERT and GAPDH were quantified as copy numbers (per reaction) using the standard curve. Finally, hTERT levels in Hela and Caski cell lines were compared quantitatively by t-test using GraphPad statistic software version 5 (San Diego, CA, USA). Results: According to the charts real-time PCR, hTERT gene expression in Hela and Caski cancer cell lines is significantly different (t=0.0319). Conclusion: All results confirm that hTERT expression levels in Hela and Caski cell lines are significantly different and the level of hTERT expression in the Caski cell line was slightly higher than that of Hela cell line. The significant difference between hTERT mRNA expression levels reported here could be used as a tumor marker for HPV16 and HPV18 in cervical cancer.http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5461&slc_lang=en&sid=1cervical cervix cancer HPV telomerase uterine cervical neoplasms |
| spellingShingle | Maryam Akhtari Mahdi Kamali Gholam Reza Javadi Seyedeh Razieh Hashemi Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virus Tehran University Medical Journal cervical cervix cancer HPV telomerase uterine cervical neoplasms |
| title | Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virus |
| title_full | Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virus |
| title_fullStr | Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virus |
| title_full_unstemmed | Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virus |
| title_short | Investigation of hTERT gene expression levels in two cell lines infected by high-risk human papilloma virus |
| title_sort | investigation of htert gene expression levels in two cell lines infected by high risk human papilloma virus |
| topic | cervical cervix cancer HPV telomerase uterine cervical neoplasms |
| url | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5461&slc_lang=en&sid=1 |
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