High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy
Oligodendrocyte loss can lead to cognitive and motor deficits. Current remyelinating therapeutic strategies imply either modulation of endogenous oligodendrocyte precursors or transplantation of in vitro expanded oligodendrocytes. Cell therapy, however, still lacks identification of an adequate sour...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2017.00703/full |
| _version_ | 1811261789753573376 |
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| author | Sissi Dolci Annachiara Pino Valeria Berton Pau Gonzalez Alice Braga Marta Fumagalli Elisabetta Bonfanti Giorgio Malpeli Francesca Pari Stefania Zorzin Clelia Amoroso Denny Moscon Francisco J. Rodriguez Guido Fumagalli Francesco Bifari Ilaria Decimo |
| author_facet | Sissi Dolci Annachiara Pino Valeria Berton Pau Gonzalez Alice Braga Marta Fumagalli Elisabetta Bonfanti Giorgio Malpeli Francesca Pari Stefania Zorzin Clelia Amoroso Denny Moscon Francisco J. Rodriguez Guido Fumagalli Francesco Bifari Ilaria Decimo |
| author_sort | Sissi Dolci |
| collection | DOAJ |
| description | Oligodendrocyte loss can lead to cognitive and motor deficits. Current remyelinating therapeutic strategies imply either modulation of endogenous oligodendrocyte precursors or transplantation of in vitro expanded oligodendrocytes. Cell therapy, however, still lacks identification of an adequate source of oligodendrocyte present in adulthood and able to efficiently produce transplantable cells. Recently, a neural stem cell-like population has been identified in meninges. We developed a protocol to obtain high yield of oligodendrocyte lineage cells from one single biopsy of adult rat meningeal tissue. From 1 cm2 of adult rat spinal cord meninges, we efficiently expanded a homogenous culture of 10 millions of meningeal-derived oligodendrocyte lineage cells in a short period of time (approximately 4 weeks). Meningeal-derived oligodendrocyte lineage cells show typical mature oligodendrocyte morphology and express specific oligodendrocyte markers, such as galactosylceramidase and myelin basic protein. Moreover, when transplanted in a chemically demyelinated spinal cord model, meningeal-derived oligodendrocyte lineage cells display in vivo-remyelinating potential. This oligodendrocyte lineage cell population derives from an accessible and adult source, being therefore a promising candidate for autologous cell therapy of demyelinating diseases. In addition, the described method to differentiate meningeal-derived neural stem cells into oligodendrocyte lineage cells may represent a valid in vitro model to dissect oligodendrocyte differentiation and to screen for drugs capable to promote oligodendrocyte regeneration. |
| first_indexed | 2024-04-12T19:12:11Z |
| format | Article |
| id | doaj.art-22bab551505c42849a406f9528df538a |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T19:12:11Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-22bab551505c42849a406f9528df538a2022-12-22T03:19:50ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122017-10-01810.3389/fphar.2017.00703295777High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal BiopsySissi Dolci0Annachiara Pino1Valeria Berton2Pau Gonzalez3Alice Braga4Marta Fumagalli5Elisabetta Bonfanti6Giorgio Malpeli7Francesca Pari8Stefania Zorzin9Clelia Amoroso10Denny Moscon11Francisco J. Rodriguez12Guido Fumagalli13Francesco Bifari14Ilaria Decimo15Section of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalyGroup of Molecular Neurology, Hospital Nacional de Parapléjicos, Toledo, SpainSection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalyLaboratory of Molecular and Cellular Pharmacology of Purinergic Transmission, Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, ItalyLaboratory of Molecular and Cellular Pharmacology of Purinergic Transmission, Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, ItalySection of General and Pancreatic Surgery, Department of Surgery, Dentistry, Paediatrics and Gynaecology, University of Verona, Verona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalyGroup of Molecular Neurology, Hospital Nacional de Parapléjicos, Toledo, SpainSection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalyLaboratory of Cell Metabolism and Regenerative Medicine, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of Verona, Verona, ItalyOligodendrocyte loss can lead to cognitive and motor deficits. Current remyelinating therapeutic strategies imply either modulation of endogenous oligodendrocyte precursors or transplantation of in vitro expanded oligodendrocytes. Cell therapy, however, still lacks identification of an adequate source of oligodendrocyte present in adulthood and able to efficiently produce transplantable cells. Recently, a neural stem cell-like population has been identified in meninges. We developed a protocol to obtain high yield of oligodendrocyte lineage cells from one single biopsy of adult rat meningeal tissue. From 1 cm2 of adult rat spinal cord meninges, we efficiently expanded a homogenous culture of 10 millions of meningeal-derived oligodendrocyte lineage cells in a short period of time (approximately 4 weeks). Meningeal-derived oligodendrocyte lineage cells show typical mature oligodendrocyte morphology and express specific oligodendrocyte markers, such as galactosylceramidase and myelin basic protein. Moreover, when transplanted in a chemically demyelinated spinal cord model, meningeal-derived oligodendrocyte lineage cells display in vivo-remyelinating potential. This oligodendrocyte lineage cell population derives from an accessible and adult source, being therefore a promising candidate for autologous cell therapy of demyelinating diseases. In addition, the described method to differentiate meningeal-derived neural stem cells into oligodendrocyte lineage cells may represent a valid in vitro model to dissect oligodendrocyte differentiation and to screen for drugs capable to promote oligodendrocyte regeneration.http://journal.frontiersin.org/article/10.3389/fphar.2017.00703/fulloligodendrocyte precursor cellsmeningesmeningeal neural stem cellsmyelinoligodendrocyte differentiationadult neural stem cells |
| spellingShingle | Sissi Dolci Annachiara Pino Valeria Berton Pau Gonzalez Alice Braga Marta Fumagalli Elisabetta Bonfanti Giorgio Malpeli Francesca Pari Stefania Zorzin Clelia Amoroso Denny Moscon Francisco J. Rodriguez Guido Fumagalli Francesco Bifari Ilaria Decimo High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy Frontiers in Pharmacology oligodendrocyte precursor cells meninges meningeal neural stem cells myelin oligodendrocyte differentiation adult neural stem cells |
| title | High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy |
| title_full | High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy |
| title_fullStr | High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy |
| title_full_unstemmed | High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy |
| title_short | High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy |
| title_sort | high yield of adult oligodendrocyte lineage cells obtained from meningeal biopsy |
| topic | oligodendrocyte precursor cells meninges meningeal neural stem cells myelin oligodendrocyte differentiation adult neural stem cells |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2017.00703/full |
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