Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression
The relationship among microbiome, immunity and cervical cancer has been targeted by several studies, yet many questions remain unanswered. We characterized herein the virome and bacteriome from cervical samples and correlated these findings with innate immunity gene expression in a Brazilian conven...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1147950/full |
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| author | Alan Messala A. Britto Alan Messala A. Britto Alan Messala A. Britto Juliana D. Siqueira Gislaine Curty Livia R. Goes Cintia Policarpo Angela R. Meyrelles Yara Furtado Yara Furtado Gutemberg Almeida Ana Lucia M. Giannini Elizabeth S. Machado Marcelo A. Soares |
| author_facet | Alan Messala A. Britto Alan Messala A. Britto Alan Messala A. Britto Juliana D. Siqueira Gislaine Curty Livia R. Goes Cintia Policarpo Angela R. Meyrelles Yara Furtado Yara Furtado Gutemberg Almeida Ana Lucia M. Giannini Elizabeth S. Machado Marcelo A. Soares |
| author_sort | Alan Messala A. Britto |
| collection | DOAJ |
| description | The relationship among microbiome, immunity and cervical cancer has been targeted by several studies, yet many questions remain unanswered. We characterized herein the virome and bacteriome from cervical samples and correlated these findings with innate immunity gene expression in a Brazilian convenience sample of HPV-infected (HPV+) and uninfected (HPV-) women. For this purpose, innate immune gene expression data were correlated to metagenomic information. Correlation analysis showed that interferon (IFN) is able to differentially modulate pattern recognition receptors (PRRs) expression based on HPV status. Virome analysis indicated that HPV infection correlates to the presence of Anellovirus (AV) and seven complete HPV genomes were assembled. Bacteriome results unveiled that vaginal community state types (CST) distribution was independent of HPV or AV status, although bacterial phyla distribution differed between groups. Furthermore, TLR3 and IFNαR2 levels were higher in the Lactobacillus no iners-dominated mucosa and we detected correlations among RIG-like receptors (RLR) associated genes and abundance of specific anaerobic bacteria. Collectively, our data show an intriguing connection between HPV and AV infections that could foster cervical cancer development. Besides that, TLR3 and IFNαR2 seem to create a protective milieu in healthy cervical mucosa (L. no iners-dominated), and RLRs, known to recognize viral RNA, were correlated to anaerobic bacteria suggesting that they might be related to dysbiosis. |
| first_indexed | 2024-04-09T16:02:37Z |
| format | Article |
| id | doaj.art-22bd0454494b4357a9c2511ef94fcea6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-09T16:02:37Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-22bd0454494b4357a9c2511ef94fcea62023-04-25T07:56:29ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-04-011410.3389/fimmu.2023.11479501147950Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expressionAlan Messala A. Britto0Alan Messala A. Britto1Alan Messala A. Britto2Juliana D. Siqueira3Gislaine Curty4Livia R. Goes5Cintia Policarpo6Angela R. Meyrelles7Yara Furtado8Yara Furtado9Gutemberg Almeida10Ana Lucia M. Giannini11Elizabeth S. Machado12Marcelo A. Soares13Departamento de Enfermagem Materno-Infantil (DEMI), Faculdade de Enfermagem (FEnf), Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, BrazilPrograma de Oncovirologia, Instituto Nacional de Câncer (INCA), Rio de Janeiro, BrazilPrograma de Pós-Graduação em Infecção HIV/aids e Hepatites Virais, Hospital Universitário Gaffrée e Guinle (HUGG/Ebserh), Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Rio de Janeiro, BrazilPrograma de Oncovirologia, Instituto Nacional de Câncer (INCA), Rio de Janeiro, BrazilPrograma de Oncovirologia, Instituto Nacional de Câncer (INCA), Rio de Janeiro, BrazilPrograma de Oncovirologia, Instituto Nacional de Câncer (INCA), Rio de Janeiro, BrazilLaboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilInstituto de Ginecologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilInstituto de Ginecologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilEscola de Medicina e Cirurgia da Universidade Federal do Estado do Rio de Janeiro, Universidade Federal do Rio de Janeiro (UNIRIO), Rio de Janeiro, BrazilInstituto de Ginecologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Genômica Funcional e Transdução de Sinal, Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, BrazilHospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilPrograma de Oncovirologia, Instituto Nacional de Câncer (INCA), Rio de Janeiro, BrazilThe relationship among microbiome, immunity and cervical cancer has been targeted by several studies, yet many questions remain unanswered. We characterized herein the virome and bacteriome from cervical samples and correlated these findings with innate immunity gene expression in a Brazilian convenience sample of HPV-infected (HPV+) and uninfected (HPV-) women. For this purpose, innate immune gene expression data were correlated to metagenomic information. Correlation analysis showed that interferon (IFN) is able to differentially modulate pattern recognition receptors (PRRs) expression based on HPV status. Virome analysis indicated that HPV infection correlates to the presence of Anellovirus (AV) and seven complete HPV genomes were assembled. Bacteriome results unveiled that vaginal community state types (CST) distribution was independent of HPV or AV status, although bacterial phyla distribution differed between groups. Furthermore, TLR3 and IFNαR2 levels were higher in the Lactobacillus no iners-dominated mucosa and we detected correlations among RIG-like receptors (RLR) associated genes and abundance of specific anaerobic bacteria. Collectively, our data show an intriguing connection between HPV and AV infections that could foster cervical cancer development. Besides that, TLR3 and IFNαR2 seem to create a protective milieu in healthy cervical mucosa (L. no iners-dominated), and RLRs, known to recognize viral RNA, were correlated to anaerobic bacteria suggesting that they might be related to dysbiosis.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1147950/fullmicrobiome and dysbiosisinnate immunity recognitionvirome analysishuman papilloma virus (HPV)squamous intraepithelial lesion of the cervixRIG-I-like receptor family |
| spellingShingle | Alan Messala A. Britto Alan Messala A. Britto Alan Messala A. Britto Juliana D. Siqueira Gislaine Curty Livia R. Goes Cintia Policarpo Angela R. Meyrelles Yara Furtado Yara Furtado Gutemberg Almeida Ana Lucia M. Giannini Elizabeth S. Machado Marcelo A. Soares Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression Frontiers in Immunology microbiome and dysbiosis innate immunity recognition virome analysis human papilloma virus (HPV) squamous intraepithelial lesion of the cervix RIG-I-like receptor family |
| title | Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression |
| title_full | Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression |
| title_fullStr | Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression |
| title_full_unstemmed | Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression |
| title_short | Microbiome analysis of Brazilian women cervix reveals specific bacterial abundance correlation to RIG-like receptor gene expression |
| title_sort | microbiome analysis of brazilian women cervix reveals specific bacterial abundance correlation to rig like receptor gene expression |
| topic | microbiome and dysbiosis innate immunity recognition virome analysis human papilloma virus (HPV) squamous intraepithelial lesion of the cervix RIG-I-like receptor family |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1147950/full |
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