Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients
BACKGROUND AND OBJECTIVES: Resistin is an adipocytokine, which has been studied for its role in insulin resistance and recently in inflammation. Several single-nucleotide polymorphisms (SNPs) have been identified in the human resistin gene (RETN). This study aims to investigate the association of RE...
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| Format: | Article |
| Language: | English |
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King Faisal Specialist Hospital and Research Centre
2014-07-01
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| Series: | Annals of Saudi Medicine |
| Online Access: | https://www.annsaudimed.net/doi/full/10.5144/0256-4947.2014.334 |
| _version_ | 1819172495109914624 |
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| author | Rowyda N. Alharithy |
| author_facet | Rowyda N. Alharithy |
| author_sort | Rowyda N. Alharithy |
| collection | DOAJ |
| description | BACKGROUND AND OBJECTIVES: Resistin is an adipocytokine, which has been studied for its role in insulin resistance and recently in inflammation. Several single-nucleotide polymorphisms (SNPs) have been identified in the human resistin gene (RETN). This study aims to investigate the association of RETN rs1862513 (C-420G) and rs3745367 (G+299A) SNPs with the colon cancer risk in Saudi patients. DESIGN AND SETTINGS: This is a case-control study conducted among Saudi adult colon cancer patients recruited from King Abdulaziz Hospital and Oncology Center in Jeddah, Saudi Arabia. SUBJECTS AND METHODS: In this study, 120 Saudi volunteers (60 colon cancer patients and 60 disease-free controls) were studied. The SNPs were determined by polymerase chain reaction (PCR) and genotyping using PCR- restriction fragment length polymorphism analysis. RESULTS: In comparing the result obtained for the patient group with that of the controls, colon cancer group displayed different genotype distribution of the RETN C-420G and G+299A SNPs. The study indicated that the SNP-420 heterozygous (CG) genotype (odds ratio [OR]=2.48, 95% CI 1.07–5.74, P=.03) and the SNP +299 heterozygous (GA) genotype (OR=6.5, 95% CI 1.77–24.18, P=.002) significantly increased the risk of colon cancer. A further analysis of the genotype combination of SNPs RETN C-420G and G+299A showed a larger increase in the colon cancer risk. CONCLUSION: These preliminary results suggested a potential role for RETN C-420G and G+299A polymorphisms in the genetic predisposition to colon cancer disease. |
| first_indexed | 2024-12-22T20:08:05Z |
| format | Article |
| id | doaj.art-22bf51c323bc46f99bc9656d83b19700 |
| institution | Directory Open Access Journal |
| issn | 0256-4947 0975-4466 |
| language | English |
| last_indexed | 2024-12-22T20:08:05Z |
| publishDate | 2014-07-01 |
| publisher | King Faisal Specialist Hospital and Research Centre |
| record_format | Article |
| series | Annals of Saudi Medicine |
| spelling | doaj.art-22bf51c323bc46f99bc9656d83b197002022-12-21T18:14:06ZengKing Faisal Specialist Hospital and Research CentreAnnals of Saudi Medicine0256-49470975-44662014-07-0134433433910.5144/0256-4947.2014.334asm-4-334Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patientsRowyda N. Alharithy0From the Department of Biochemistry, King Abdulaziz University, Jeddah, Saudi ArabiaBACKGROUND AND OBJECTIVES: Resistin is an adipocytokine, which has been studied for its role in insulin resistance and recently in inflammation. Several single-nucleotide polymorphisms (SNPs) have been identified in the human resistin gene (RETN). This study aims to investigate the association of RETN rs1862513 (C-420G) and rs3745367 (G+299A) SNPs with the colon cancer risk in Saudi patients. DESIGN AND SETTINGS: This is a case-control study conducted among Saudi adult colon cancer patients recruited from King Abdulaziz Hospital and Oncology Center in Jeddah, Saudi Arabia. SUBJECTS AND METHODS: In this study, 120 Saudi volunteers (60 colon cancer patients and 60 disease-free controls) were studied. The SNPs were determined by polymerase chain reaction (PCR) and genotyping using PCR- restriction fragment length polymorphism analysis. RESULTS: In comparing the result obtained for the patient group with that of the controls, colon cancer group displayed different genotype distribution of the RETN C-420G and G+299A SNPs. The study indicated that the SNP-420 heterozygous (CG) genotype (odds ratio [OR]=2.48, 95% CI 1.07–5.74, P=.03) and the SNP +299 heterozygous (GA) genotype (OR=6.5, 95% CI 1.77–24.18, P=.002) significantly increased the risk of colon cancer. A further analysis of the genotype combination of SNPs RETN C-420G and G+299A showed a larger increase in the colon cancer risk. CONCLUSION: These preliminary results suggested a potential role for RETN C-420G and G+299A polymorphisms in the genetic predisposition to colon cancer disease.https://www.annsaudimed.net/doi/full/10.5144/0256-4947.2014.334 |
| spellingShingle | Rowyda N. Alharithy Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients Annals of Saudi Medicine |
| title | Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients |
| title_full | Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients |
| title_fullStr | Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients |
| title_full_unstemmed | Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients |
| title_short | Polymorphisms in RETN gene and susceptibility to colon cancer in Saudi patients |
| title_sort | polymorphisms in retn gene and susceptibility to colon cancer in saudi patients |
| url | https://www.annsaudimed.net/doi/full/10.5144/0256-4947.2014.334 |
| work_keys_str_mv | AT rowydanalharithy polymorphismsinretngeneandsusceptibilitytocoloncancerinsaudipatients |