Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ
The gene expression and protein synthesis of small leucine-rich proteoglycans (SLRPs), including decorin, biglycan, fibromodulin, and lumican, was analyzed in the context of the hypothesis that they are closely related to tooth formation. In situ hybridization, immunohistochemistry, and organ cultur...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2020-02-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/3092 |
| _version_ | 1818059292506324992 |
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| author | Angammana Randilini Kaoru Fujikawa Shunichi Shibata |
| author_facet | Angammana Randilini Kaoru Fujikawa Shunichi Shibata |
| author_sort | Angammana Randilini |
| collection | DOAJ |
| description | The gene expression and protein synthesis of small leucine-rich proteoglycans (SLRPs), including decorin, biglycan, fibromodulin, and lumican, was analyzed in the context of the hypothesis that they are closely related to tooth formation. In situ hybridization, immunohistochemistry, and organ culture with metabolic labeling of [35S] were carried out in mouse first molar tooth germs of different developmental stages using ICR mice at embryonic day (E) 13.5 to postnatal day (P) 7.0. At the bud and cap stage, decorin mRNA was expressed only in the surrounding mesenchyme, but not within the tooth germ. Biglycan mRNA was then expressed in the condensing mesenchyme and the dental papilla of the tooth germ. At the apposition stage (late bell stage), both decorin and biglycan mRNA were expressed in odontoblasts, resulting in a switch of the pattern of expression within the different stages of odontoblast differentiation. Decorin mRNA was expressed earlier in newly differentiating odontoblasts than biglycan. With odontoblast maturation and dentin formation, decorin mRNA expression was diminished and localized to the newly differentiating odontoblasts at the cervical region. Simultaneously, biglycan mRNA took over and extended its expression throughout the new and mature odontoblasts. Both mRNAs were expressed in the dental pulp underlying the respective odontoblasts. At P7.0, both mRNAs were weakly expressed but maintained their spatial expression patterns. Immunostaining showed that biglycan was localized in the dental papillae and pulp. In addition, all four SLRPs showed clear immunostaining in predentin, although the expressions of fibromodulin and lumican mRNAs were not identified in the tooth germs examined. The organ culture data obtained supported the histological findings that biglycan is more predominant than decorin at the apposition stage. These results were used to identify biglycan as the principal molecule among the SLRPs investigated. Our findings indicate that decorin and biglycan show spatial and temporal differential expressions and play their own tissue-specific roles in tooth development. |
| first_indexed | 2024-12-10T13:14:12Z |
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| id | doaj.art-22c1b59295c5406b9ed6d5ca67198ee5 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-10T13:14:12Z |
| publishDate | 2020-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-22c1b59295c5406b9ed6d5ca67198ee52022-12-22T01:47:36ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062020-02-0164110.4081/ejh.2020.3092Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germAngammana Randilini0Kaoru Fujikawa1Shunichi Shibata2Department of Maxillofacial Anatomy, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo Department of Oral Anatomy and Developmental Biology, Showa University School of Dentistry, TokyoDepartment of Maxillofacial Anatomy, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo The gene expression and protein synthesis of small leucine-rich proteoglycans (SLRPs), including decorin, biglycan, fibromodulin, and lumican, was analyzed in the context of the hypothesis that they are closely related to tooth formation. In situ hybridization, immunohistochemistry, and organ culture with metabolic labeling of [35S] were carried out in mouse first molar tooth germs of different developmental stages using ICR mice at embryonic day (E) 13.5 to postnatal day (P) 7.0. At the bud and cap stage, decorin mRNA was expressed only in the surrounding mesenchyme, but not within the tooth germ. Biglycan mRNA was then expressed in the condensing mesenchyme and the dental papilla of the tooth germ. At the apposition stage (late bell stage), both decorin and biglycan mRNA were expressed in odontoblasts, resulting in a switch of the pattern of expression within the different stages of odontoblast differentiation. Decorin mRNA was expressed earlier in newly differentiating odontoblasts than biglycan. With odontoblast maturation and dentin formation, decorin mRNA expression was diminished and localized to the newly differentiating odontoblasts at the cervical region. Simultaneously, biglycan mRNA took over and extended its expression throughout the new and mature odontoblasts. Both mRNAs were expressed in the dental pulp underlying the respective odontoblasts. At P7.0, both mRNAs were weakly expressed but maintained their spatial expression patterns. Immunostaining showed that biglycan was localized in the dental papillae and pulp. In addition, all four SLRPs showed clear immunostaining in predentin, although the expressions of fibromodulin and lumican mRNAs were not identified in the tooth germs examined. The organ culture data obtained supported the histological findings that biglycan is more predominant than decorin at the apposition stage. These results were used to identify biglycan as the principal molecule among the SLRPs investigated. Our findings indicate that decorin and biglycan show spatial and temporal differential expressions and play their own tissue-specific roles in tooth development.https://www.ejh.it/index.php/ejh/article/view/3092Decorinbiglycantooth germin situ hybridizationorgan culture |
| spellingShingle | Angammana Randilini Kaoru Fujikawa Shunichi Shibata Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ European Journal of Histochemistry Decorin biglycan tooth germ in situ hybridization organ culture |
| title | Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ |
| title_full | Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ |
| title_fullStr | Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ |
| title_full_unstemmed | Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ |
| title_short | Expression, localization and synthesis of small leucine-rich proteoglycans in developing mouse molar tooth germ |
| title_sort | expression localization and synthesis of small leucine rich proteoglycans in developing mouse molar tooth germ |
| topic | Decorin biglycan tooth germ in situ hybridization organ culture |
| url | https://www.ejh.it/index.php/ejh/article/view/3092 |
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