A universal mammalian vaccine cell line substrate.
Using genome-wide small interfering RNA (siRNA) screens for poliovirus, influenza A virus and rotavirus, we validated the top 6 gene hits PV, RV or IAV to search for host genes that when knocked-down (KD) enhanced virus permissiveness and replication over wild type Vero cells or HEp-2 cells. The enh...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5703543?pdf=render |
| _version_ | 1818965351006732288 |
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| author | Jackelyn Murray Kyle V Todd Abhijeet Bakre Nichole Orr-Burks Les Jones Weilin Wu Ralph A Tripp |
| author_facet | Jackelyn Murray Kyle V Todd Abhijeet Bakre Nichole Orr-Burks Les Jones Weilin Wu Ralph A Tripp |
| author_sort | Jackelyn Murray |
| collection | DOAJ |
| description | Using genome-wide small interfering RNA (siRNA) screens for poliovirus, influenza A virus and rotavirus, we validated the top 6 gene hits PV, RV or IAV to search for host genes that when knocked-down (KD) enhanced virus permissiveness and replication over wild type Vero cells or HEp-2 cells. The enhanced virus replication was tested for 12 viruses and ranged from 2-fold to >1000-fold. There were variations in virus-specific replication (strain differences) across the cell lines examined. Some host genes (CNTD2, COQ9, GCGR, NDUFA9, NEU2, PYCR1, SEC16G, SVOPL, ZFYVE9, and ZNF205) showed that KD resulted in enhanced virus replication. These findings advance platform-enabling vaccine technology, the creation of diagnostic cells substrates, and are informative about the host mechanisms that affect virus replication in mammalian cells. |
| first_indexed | 2024-12-20T13:15:37Z |
| format | Article |
| id | doaj.art-22c465276bd349eabcdba16cd58ffb27 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T13:15:37Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-22c465276bd349eabcdba16cd58ffb272022-12-21T19:39:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018833310.1371/journal.pone.0188333A universal mammalian vaccine cell line substrate.Jackelyn MurrayKyle V ToddAbhijeet BakreNichole Orr-BurksLes JonesWeilin WuRalph A TrippUsing genome-wide small interfering RNA (siRNA) screens for poliovirus, influenza A virus and rotavirus, we validated the top 6 gene hits PV, RV or IAV to search for host genes that when knocked-down (KD) enhanced virus permissiveness and replication over wild type Vero cells or HEp-2 cells. The enhanced virus replication was tested for 12 viruses and ranged from 2-fold to >1000-fold. There were variations in virus-specific replication (strain differences) across the cell lines examined. Some host genes (CNTD2, COQ9, GCGR, NDUFA9, NEU2, PYCR1, SEC16G, SVOPL, ZFYVE9, and ZNF205) showed that KD resulted in enhanced virus replication. These findings advance platform-enabling vaccine technology, the creation of diagnostic cells substrates, and are informative about the host mechanisms that affect virus replication in mammalian cells.http://europepmc.org/articles/PMC5703543?pdf=render |
| spellingShingle | Jackelyn Murray Kyle V Todd Abhijeet Bakre Nichole Orr-Burks Les Jones Weilin Wu Ralph A Tripp A universal mammalian vaccine cell line substrate. PLoS ONE |
| title | A universal mammalian vaccine cell line substrate. |
| title_full | A universal mammalian vaccine cell line substrate. |
| title_fullStr | A universal mammalian vaccine cell line substrate. |
| title_full_unstemmed | A universal mammalian vaccine cell line substrate. |
| title_short | A universal mammalian vaccine cell line substrate. |
| title_sort | universal mammalian vaccine cell line substrate |
| url | http://europepmc.org/articles/PMC5703543?pdf=render |
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