L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis
High sulfides concentrations can be poisonous to environment because of anthropogenic waste production or natural occurrences. How to elucidate the biological transformation mechanisms of sulfide pollutants in the subtropical marine mangrove ecosystem has gained increased interest. Thus, in the pres...
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MDPI AG
2021-11-01
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| author | Shuming Mo Jinhui Li Bin Li Muhammad Kashif Shiqing Nie Jianping Liao Guijiao Su Qiong Jiang Bing Yan Chengjian Jiang |
| author_facet | Shuming Mo Jinhui Li Bin Li Muhammad Kashif Shiqing Nie Jianping Liao Guijiao Su Qiong Jiang Bing Yan Chengjian Jiang |
| author_sort | Shuming Mo |
| collection | DOAJ |
| description | High sulfides concentrations can be poisonous to environment because of anthropogenic waste production or natural occurrences. How to elucidate the biological transformation mechanisms of sulfide pollutants in the subtropical marine mangrove ecosystem has gained increased interest. Thus, in the present study, the sulfide biotransformation in subtropical mangroves ecosystem was accurately evaluated using metagenomic sequencing and quantitative polymerase chain reaction analysis. Most abundant genes were related to the organic sulfur transformation. Furthermore, an ecological model of sulfide conversion was constructed. Total phosphorus was the dominant environmental factor that drove the sulfur cycle and microbial communities. We compared mangrove and non-mangrove soils and found that the former enhanced metabolism that was related to sulfate reduction when compared to the latter. Total organic carbon, total organic nitrogen, iron, and available sulfur were the key environmental factors that effectively influenced the dissimilatory sulfate reduction. The taxonomic assignment of dissimilatory sulfate-reducing genes revealed that <i>Desulfobacterales</i> and <i>Chromatiales</i> were mainly responsible for sulfate reduction. <i>Chromatiales</i> were most sensitive to environmental factors. The high abundance of <i>cysE</i> and <i>cysK</i> could contribute to the coping of the microbial community with the toxic sulfide produced by <i>Desulfobacterales</i>. Collectively, these findings provided a theoretical basis for the mechanism of the sulfur cycle in subtropical mangrove ecosystems. |
| first_indexed | 2024-03-10T05:48:47Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4441 |
| language | English |
| last_indexed | 2024-03-10T05:48:47Z |
| publishDate | 2021-11-01 |
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| record_format | Article |
| series | Water |
| spelling | doaj.art-22c58d4cd50c4caca895aa2269201b1d2023-11-22T21:54:34ZengMDPI AGWater2073-44412021-11-011321305310.3390/w13213053L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics AnalysisShuming Mo0Jinhui Li1Bin Li2Muhammad Kashif3Shiqing Nie4Jianping Liao5Guijiao Su6Qiong Jiang7Bing Yan8Chengjian Jiang9State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai 536000, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaSchool of Computer and Information Engineering, Nanning Normal University, Nanning 530299, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Sciences, Beihai 536000, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaHigh sulfides concentrations can be poisonous to environment because of anthropogenic waste production or natural occurrences. How to elucidate the biological transformation mechanisms of sulfide pollutants in the subtropical marine mangrove ecosystem has gained increased interest. Thus, in the present study, the sulfide biotransformation in subtropical mangroves ecosystem was accurately evaluated using metagenomic sequencing and quantitative polymerase chain reaction analysis. Most abundant genes were related to the organic sulfur transformation. Furthermore, an ecological model of sulfide conversion was constructed. Total phosphorus was the dominant environmental factor that drove the sulfur cycle and microbial communities. We compared mangrove and non-mangrove soils and found that the former enhanced metabolism that was related to sulfate reduction when compared to the latter. Total organic carbon, total organic nitrogen, iron, and available sulfur were the key environmental factors that effectively influenced the dissimilatory sulfate reduction. The taxonomic assignment of dissimilatory sulfate-reducing genes revealed that <i>Desulfobacterales</i> and <i>Chromatiales</i> were mainly responsible for sulfate reduction. <i>Chromatiales</i> were most sensitive to environmental factors. The high abundance of <i>cysE</i> and <i>cysK</i> could contribute to the coping of the microbial community with the toxic sulfide produced by <i>Desulfobacterales</i>. Collectively, these findings provided a theoretical basis for the mechanism of the sulfur cycle in subtropical mangrove ecosystems.https://www.mdpi.com/2073-4441/13/21/3053sulfate-reduction gene familiessubtropical mangrove sedimentsulfidemetagenomicsL-cysteine synthase |
| spellingShingle | Shuming Mo Jinhui Li Bin Li Muhammad Kashif Shiqing Nie Jianping Liao Guijiao Su Qiong Jiang Bing Yan Chengjian Jiang L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis Water sulfate-reduction gene families subtropical mangrove sediment sulfide metagenomics L-cysteine synthase |
| title | L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis |
| title_full | L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis |
| title_fullStr | L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis |
| title_full_unstemmed | L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis |
| title_short | L-Cysteine Synthase Enhanced Sulfide Biotransformation in Subtropical Marine Mangrove Sediments as Revealed by Metagenomics Analysis |
| title_sort | l cysteine synthase enhanced sulfide biotransformation in subtropical marine mangrove sediments as revealed by metagenomics analysis |
| topic | sulfate-reduction gene families subtropical mangrove sediment sulfide metagenomics L-cysteine synthase |
| url | https://www.mdpi.com/2073-4441/13/21/3053 |
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