Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry
Nano-carrier systems such as liposomes have promising biomedical applications. Nevertheless, characterization of these complex samples is a challenging analytical task. In this study a coupled hydrodynamic chromatography-single particle-inductively coupled plasma mass spectrometry (HDC-spICP-MS) app...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/13/6/1447 |
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| author | Yves U. Hachenberger Daniel Rosenkranz Fabian L. Kriegel Benjamin Krause René Matschaß Philipp Reichardt Jutta Tentschert Peter Laux Norbert Jakubowski Ulrich Panne Andreas Luch |
| author_facet | Yves U. Hachenberger Daniel Rosenkranz Fabian L. Kriegel Benjamin Krause René Matschaß Philipp Reichardt Jutta Tentschert Peter Laux Norbert Jakubowski Ulrich Panne Andreas Luch |
| author_sort | Yves U. Hachenberger |
| collection | DOAJ |
| description | Nano-carrier systems such as liposomes have promising biomedical applications. Nevertheless, characterization of these complex samples is a challenging analytical task. In this study a coupled hydrodynamic chromatography-single particle-inductively coupled plasma mass spectrometry (HDC-spICP-MS) approach was validated based on the technical specification (TS) 19590:2017 of the international organization for standardization (ISO). The TS has been adapted to the hyphenated setup. The quality criteria (QC), e.g., linearity of the calibration, transport efficiency, were investigated. Furthermore, a cross calibration of the particle size was performed with values from dynamic light scattering (DLS) and transmission electron microscopy (TEM). Due to an additional Y-piece, an online-calibration routine was implemented. This approach allows the calibration of the ICP-MS during the dead time of the chromatography run, to reduce the required time and enhance the robustness of the results. The optimized method was tested with different gold nanoparticle (Au-NP) mixtures to investigate the characterization properties of HDC separations for samples with increasing complexity. Additionally, the technique was successfully applied to simultaneously determine both the hydrodynamic radius and the Au-NP content in liposomes. With the established hyphenated setup, it was possible to distinguish between different subpopulations with various NP loads and different hydrodynamic diameters inside the liposome carriers. |
| first_indexed | 2024-04-13T09:57:14Z |
| format | Article |
| id | doaj.art-22c6dba83d4749b691679e1a22674507 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-04-13T09:57:14Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-22c6dba83d4749b691679e1a226745072022-12-22T02:51:21ZengMDPI AGMaterials1996-19442020-03-01136144710.3390/ma13061447ma13061447Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass SpectrometryYves U. Hachenberger0Daniel Rosenkranz1Fabian L. Kriegel2Benjamin Krause3René Matschaß4Philipp Reichardt5Jutta Tentschert6Peter Laux7Norbert Jakubowski8Ulrich Panne9Andreas Luch10Department of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanySPETEC GmbH, Berghamer Str. 2, 85435 Erding, GermanyFederal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Strasse 11, 12489 Berlin, GermanyDepartment of Chemical & Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin, GermanyNano-carrier systems such as liposomes have promising biomedical applications. Nevertheless, characterization of these complex samples is a challenging analytical task. In this study a coupled hydrodynamic chromatography-single particle-inductively coupled plasma mass spectrometry (HDC-spICP-MS) approach was validated based on the technical specification (TS) 19590:2017 of the international organization for standardization (ISO). The TS has been adapted to the hyphenated setup. The quality criteria (QC), e.g., linearity of the calibration, transport efficiency, were investigated. Furthermore, a cross calibration of the particle size was performed with values from dynamic light scattering (DLS) and transmission electron microscopy (TEM). Due to an additional Y-piece, an online-calibration routine was implemented. This approach allows the calibration of the ICP-MS during the dead time of the chromatography run, to reduce the required time and enhance the robustness of the results. The optimized method was tested with different gold nanoparticle (Au-NP) mixtures to investigate the characterization properties of HDC separations for samples with increasing complexity. Additionally, the technique was successfully applied to simultaneously determine both the hydrodynamic radius and the Au-NP content in liposomes. With the established hyphenated setup, it was possible to distinguish between different subpopulations with various NP loads and different hydrodynamic diameters inside the liposome carriers.https://www.mdpi.com/1996-1944/13/6/1447single particle icp-msnanoparticle characterizationnano-carrierliposomeshydrodynamic chromatography (hdc)validation |
| spellingShingle | Yves U. Hachenberger Daniel Rosenkranz Fabian L. Kriegel Benjamin Krause René Matschaß Philipp Reichardt Jutta Tentschert Peter Laux Norbert Jakubowski Ulrich Panne Andreas Luch Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry Materials single particle icp-ms nanoparticle characterization nano-carrier liposomes hydrodynamic chromatography (hdc) validation |
| title | Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry |
| title_full | Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry |
| title_fullStr | Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry |
| title_full_unstemmed | Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry |
| title_short | Tackling Complex Analytical Tasks: An ISO/TS-Based Validation Approach for Hydrodynamic Chromatography Single Particle Inductively Coupled Plasma Mass Spectrometry |
| title_sort | tackling complex analytical tasks an iso ts based validation approach for hydrodynamic chromatography single particle inductively coupled plasma mass spectrometry |
| topic | single particle icp-ms nanoparticle characterization nano-carrier liposomes hydrodynamic chromatography (hdc) validation |
| url | https://www.mdpi.com/1996-1944/13/6/1447 |
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