<i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome
Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of <i>Lactiplantibacillus plantarum&...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/23/12665 |
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| author | Young-Sil Lee Eun-Jung Park Gun-Seok Park Seung-Hyun Ko Juyi Park You-Kyung Lee Jong-Yeon Kim Daeyoung Lee Jihee Kang Hae-Jeung Lee |
| author_facet | Young-Sil Lee Eun-Jung Park Gun-Seok Park Seung-Hyun Ko Juyi Park You-Kyung Lee Jong-Yeon Kim Daeyoung Lee Jihee Kang Hae-Jeung Lee |
| author_sort | Young-Sil Lee |
| collection | DOAJ |
| description | Obesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of <i>Lactiplantibacillus plantarum</i> ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of <i>L. plantarum</i> ATG-K2. Mice were fed a normal fat diet or HFD with <i>L. plantarum</i> ATG-K2 and Orlistat for 8 weeks. <i>L. plantarum</i> ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPARγ, SREBP1c, and FAS and upregulation of PPARα, CTP1, UCP1, Prdm16, and ND5. Moreover, <i>L. plantarum</i> ATG-K2 decreased TG, T-CHO, leptin, and TNF-α levels in the serum, with corresponding gene expression levels in the intestine. <i>L. plantarum</i> ATG-K2 modulated the gut microbiome by increasing the abundance of the <i>Lactobacillaceae</i> family, which increased SCFA levels and branched SCFAs in the feces. <i>L. plantarum</i> ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore, <i>L. plantarum</i> ATG-K2 can be a preventive and therapeutic agent for obesity. |
| first_indexed | 2024-03-10T04:53:01Z |
| format | Article |
| id | doaj.art-22c7f143e295447bad5b7ef74eccdae6 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:53:01Z |
| publishDate | 2021-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-22c7f143e295447bad5b7ef74eccdae62023-11-23T02:26:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122231266510.3390/ijms222312665<i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut MicrobiomeYoung-Sil Lee0Eun-Jung Park1Gun-Seok Park2Seung-Hyun Ko3Juyi Park4You-Kyung Lee5Jong-Yeon Kim6Daeyoung Lee7Jihee Kang8Hae-Jeung Lee9AtoGen Co., Ltd., Daejeon 34015, KoreaDepartment of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, KoreaAtoGen Co., Ltd., Daejeon 34015, KoreaAtoGen Co., Ltd., Daejeon 34015, KoreaAtoGen Co., Ltd., Daejeon 34015, KoreaAtoGen Co., Ltd., Daejeon 34015, KoreaDepartment of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, KoreaAtoGen Co., Ltd., Daejeon 34015, KoreaAtoGen Co., Ltd., Daejeon 34015, KoreaDepartment of Food and Nutrition, College of BioNano Technology, Gachon University, Gyeonggi-do 13120, KoreaObesity is a major health problem. Compelling evidence supports the beneficial effects of probiotics on obesity. However, the anti-obesity effect of probiotics remains unknown. In this study, we investigated the anti-obesity effects and potential mechanisms of <i>Lactiplantibacillus plantarum</i> ATG-K2 using 3T3-L1 adipocytes and high-fat diet (HFD)-induced obese mice. 3T3-L1 cells were incubated to determine the effect of lipid accumulation with lysate of <i>L. plantarum</i> ATG-K2. Mice were fed a normal fat diet or HFD with <i>L. plantarum</i> ATG-K2 and Orlistat for 8 weeks. <i>L. plantarum</i> ATG-K2 inhibited lipid accumulation in 3T3-L1 adipocytes, and reduced body weight gain, WAT weight, and adipocyte size in HFD-induced obese mice, concurrently with the downregulation of PPARγ, SREBP1c, and FAS and upregulation of PPARα, CTP1, UCP1, Prdm16, and ND5. Moreover, <i>L. plantarum</i> ATG-K2 decreased TG, T-CHO, leptin, and TNF-α levels in the serum, with corresponding gene expression levels in the intestine. <i>L. plantarum</i> ATG-K2 modulated the gut microbiome by increasing the abundance of the <i>Lactobacillaceae</i> family, which increased SCFA levels and branched SCFAs in the feces. <i>L. plantarum</i> ATG-K2 exhibited an anti-obesity effect and anti-hyperlipidemic effect in 3T3-L1 adipocytes and HFD-induced obese mice by alleviating the inflammatory response and regulating lipid metabolism, which may be influenced by modulation of the gut microbiome and its metabolites. Therefore, <i>L. plantarum</i> ATG-K2 can be a preventive and therapeutic agent for obesity.https://www.mdpi.com/1422-0067/22/23/12665obesityprobiotics<i>Lactiplantibacillus plantarum</i> ATG-K2gut microbiomeshort-chain fatty acidslipid metabolism |
| spellingShingle | Young-Sil Lee Eun-Jung Park Gun-Seok Park Seung-Hyun Ko Juyi Park You-Kyung Lee Jong-Yeon Kim Daeyoung Lee Jihee Kang Hae-Jeung Lee <i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome International Journal of Molecular Sciences obesity probiotics <i>Lactiplantibacillus plantarum</i> ATG-K2 gut microbiome short-chain fatty acids lipid metabolism |
| title | <i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome |
| title_full | <i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome |
| title_fullStr | <i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome |
| title_full_unstemmed | <i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome |
| title_short | <i>Lactiplantibacillus</i><i>plantarum</i> ATG-K2 Exerts an Anti-Obesity Effect in High-Fat Diet-Induced Obese Mice by Modulating the Gut Microbiome |
| title_sort | i lactiplantibacillus i i plantarum i atg k2 exerts an anti obesity effect in high fat diet induced obese mice by modulating the gut microbiome |
| topic | obesity probiotics <i>Lactiplantibacillus plantarum</i> ATG-K2 gut microbiome short-chain fatty acids lipid metabolism |
| url | https://www.mdpi.com/1422-0067/22/23/12665 |
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