Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline Products
Cordyceps, a parasitic complex of the fungus <i>Ophiocordyceps sinensis</i> (Berk.) (Hypocreales: Ophiocordycipitaceae) and the ghost moth <i>Thitarodes</i> (Lepidoptera: Hepialidae), is a historical ethnopharmacological commodity in China. Recently, artificial cultivation of...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/8/666 |
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| author | Rui Tang Xue-Hong Qiu Li Cao Hai-Lin Long Ri-Chou Han |
| author_facet | Rui Tang Xue-Hong Qiu Li Cao Hai-Lin Long Ri-Chou Han |
| author_sort | Rui Tang |
| collection | DOAJ |
| description | Cordyceps, a parasitic complex of the fungus <i>Ophiocordyceps sinensis</i> (Berk.) (Hypocreales: Ophiocordycipitaceae) and the ghost moth <i>Thitarodes</i> (Lepidoptera: Hepialidae), is a historical ethnopharmacological commodity in China. Recently, artificial cultivation of Chinese cordyceps has been established to supplement the dwindling natural resources. However, much is unknown between the natural and cultivated products in terms of nutritional aspect, which may provide essential information for quality evaluation. The current study aims to determine the metabolic profiles of 17 treatments from 3 sample groups including <i>O. sinensis</i> fungus, <i>Thitarodes</i> insect and cordyceps complex, using Gas Chromatography - Quadrupole Time-of-Flight Mass Spectrometry. A total of 98 metabolites were detected, with 90 of them varying in concentrations among groups. The tested groups could be separated, except that fungal fruiting body was clustered into the same group as Chinese cordyceps. The main distinguishing factors for the groups studied were the 24 metabolites involved in numerous different metabolic pathways. In conclusion, metabolomics of <i>O. sinensis</i> and its related products were determined mainly by the fruiting bodies other than culture methods. Our results suggest that artificially cultured fruiting bodies and cordyceps may share indistinguishable metabolic functions as the natural ones. |
| first_indexed | 2024-03-10T08:42:37Z |
| format | Article |
| id | doaj.art-22cbf10e1e274271b5f0387e4b5ed23d |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T08:42:37Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-22cbf10e1e274271b5f0387e4b5ed23d2023-11-22T08:06:44ZengMDPI AGInsects2075-44502021-07-0112866610.3390/insects12080666Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline ProductsRui Tang0Xue-Hong Qiu1Li Cao2Hai-Lin Long3Ri-Chou Han4Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaCordyceps, a parasitic complex of the fungus <i>Ophiocordyceps sinensis</i> (Berk.) (Hypocreales: Ophiocordycipitaceae) and the ghost moth <i>Thitarodes</i> (Lepidoptera: Hepialidae), is a historical ethnopharmacological commodity in China. Recently, artificial cultivation of Chinese cordyceps has been established to supplement the dwindling natural resources. However, much is unknown between the natural and cultivated products in terms of nutritional aspect, which may provide essential information for quality evaluation. The current study aims to determine the metabolic profiles of 17 treatments from 3 sample groups including <i>O. sinensis</i> fungus, <i>Thitarodes</i> insect and cordyceps complex, using Gas Chromatography - Quadrupole Time-of-Flight Mass Spectrometry. A total of 98 metabolites were detected, with 90 of them varying in concentrations among groups. The tested groups could be separated, except that fungal fruiting body was clustered into the same group as Chinese cordyceps. The main distinguishing factors for the groups studied were the 24 metabolites involved in numerous different metabolic pathways. In conclusion, metabolomics of <i>O. sinensis</i> and its related products were determined mainly by the fruiting bodies other than culture methods. Our results suggest that artificially cultured fruiting bodies and cordyceps may share indistinguishable metabolic functions as the natural ones.https://www.mdpi.com/2075-4450/12/8/666caterpillar fungus<i>Ophiocordyceps sinensis</i><i>Thitarodes</i>metabolitesGC-QTOFMS |
| spellingShingle | Rui Tang Xue-Hong Qiu Li Cao Hai-Lin Long Ri-Chou Han Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline Products Insects caterpillar fungus <i>Ophiocordyceps sinensis</i> <i>Thitarodes</i> metabolites GC-QTOFMS |
| title | Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline Products |
| title_full | Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline Products |
| title_fullStr | Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline Products |
| title_full_unstemmed | Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline Products |
| title_short | Stage- and Rearing-Dependent Metabolomics Profiling of <i>Ophiocordyceps sinensis</i> and Its Pipeline Products |
| title_sort | stage and rearing dependent metabolomics profiling of i ophiocordyceps sinensis i and its pipeline products |
| topic | caterpillar fungus <i>Ophiocordyceps sinensis</i> <i>Thitarodes</i> metabolites GC-QTOFMS |
| url | https://www.mdpi.com/2075-4450/12/8/666 |
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