A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma
Background: Laryngeal squamous cell carcinoma (LSCC) is a common cancer of the head and neck in humans. The 5-years survival rate of patients with LSCC have declined in the past four decades. microRNAs (miRNAs) has been reported to be capable of predicting the prognosis outcomes of patients with dif...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-12-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054023000483 |
| _version_ | 1797401668710563840 |
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| author | Linshi Zhang Zhe Zhang Xiwang Zheng Yan Lu Li Dai Wenqi Li Hui Liu Shuxin Wen Qiuping Xie Xiangmin Zhang Ping Wang Yongyan Wu Wei Gao |
| author_facet | Linshi Zhang Zhe Zhang Xiwang Zheng Yan Lu Li Dai Wenqi Li Hui Liu Shuxin Wen Qiuping Xie Xiangmin Zhang Ping Wang Yongyan Wu Wei Gao |
| author_sort | Linshi Zhang |
| collection | DOAJ |
| description | Background: Laryngeal squamous cell carcinoma (LSCC) is a common cancer of the head and neck in humans. The 5-years survival rate of patients with LSCC have declined in the past four decades. microRNAs (miRNAs) has been reported to be capable of predicting the prognosis outcomes of patients with different cancers. However, there are no reports on the usage of multi-miRNAs model as signature for the diagnosis or prognosis of LSCC. Methods: To establish the miRNAs expression-associated model for diagnosis, prognosis prediction and aided therapy of patients with LSCC, the present study enrolled 107 patients with LSCC in clinic and obtained 117 LSCC samples data from TCGA database for evaluation, respectively. Next generation sequencing (NGS), raw data processing, the least absolute shrinkage and selection operator algorithm, Cox regression analysis, construction of nomogram and cell function assays (including proliferation, migration and invasion assays) were sequentially performed. Results: There were massively dysregulated miRNAs in the LSCC compared to normal tissues. A six-miRNAs signature consists of miR-137–3p, miR-3934–5p, miR-1276, miR-129–5p, miR-7-5p and miR-105–5p was built for prognosis prediction of LSCC patients. The six-miRNAs signature is strongly associated with the poor overall survival (OS, p = 2.5e-05, HR: 4.30 [2.20–8.50]), progression free interval (PFI, p = 0.025, HR: 1.94 [1.08–3.46]) and disease specific survival (DSS, p = 1.1e-05, HR: 5.00 [2.50–10.00]). A nomogram for prediction of 2-, 3- and 5-years OS was also developed based on the six-miRNAs signature and clinical features. Furthermore, blocking the function of each of the six miRNAs inhibited proliferation, invasion and migration of LSCC cells. Conclusions: The performance of six-miRNAs signature described in the current study demonstrated remarkable potential for progression assessment of LSCC. Moreover, the six-miRNAs signature may serve as predictive tool for prognosis and therapeutic targets of LSCC in clinic. |
| first_indexed | 2024-03-09T02:13:24Z |
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| id | doaj.art-22cc214d0bbf4d88abbd3b9ccb92462a |
| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-03-09T02:13:24Z |
| publishDate | 2023-12-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Non-coding RNA Research |
| spelling | doaj.art-22cc214d0bbf4d88abbd3b9ccb92462a2023-12-07T05:29:54ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402023-12-0184550561A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinomaLinshi Zhang0Zhe Zhang1Xiwang Zheng2Yan Lu3Li Dai4Wenqi Li5Hui Liu6Shuxin Wen7Qiuping Xie8Xiangmin Zhang9Ping Wang10Yongyan Wu11Wei Gao12Department of Thyroid Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, PR ChinaShanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, PR China; Shenzhen Eye Institute, Shenzhen Eye Hospital, Shenzhen, 518040, Guangdong, PR ChinaShanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, PR ChinaDepartment of Otolaryngology Head & Neck Surgery, First Hospital of Jinzhou Medical University, Jinzhou, 121011, Liaoning, PR ChinaDepartment of Otolaryngology Head & Neck Surgery, Shanxi Bethune Hospital, Taiyuan, 030032, Shanxi, PR ChinaDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, Guangdong, PR ChinaDepartment of Hepatobiliary Surgery, Shenzhen University General Hospital & Shenzhen University Clinical Medical Academy Center, Shenzhen University, Shenzhen, 518055, Guangdong, PR ChinaDepartment of Otolaryngology Head & Neck Surgery, Shanxi Bethune Hospital, Taiyuan, 030032, Shanxi, PR ChinaDepartment of Thyroid Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, PR ChinaDepartment of Otolaryngology Head & Neck Surgery, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR China; Shenzhen Institute of Otolaryngology & Key Laboratory of Otolaryngology, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR ChinaDepartment of Thyroid Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, PR China; Corresponding author. Department of Thyroid Surgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, 310009, Zhejiang, PR China.Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, PR China; Department of Otolaryngology Head & Neck Surgery, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR China; Shenzhen Institute of Otolaryngology & Key Laboratory of Otolaryngology, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR China; Corresponding author. Department of Otolaryngology Head & Neck Surgery, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR China.Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, 030001, Shanxi, PR China; Department of Otolaryngology Head & Neck Surgery, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR China; Shenzhen Institute of Otolaryngology & Key Laboratory of Otolaryngology, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR China; Corresponding author. Department of Otolaryngology Head & Neck Surgery, Longgang Ear-Nose-Throat Hospital, Shenzhen, 518172, Guangdong, PR ChinaBackground: Laryngeal squamous cell carcinoma (LSCC) is a common cancer of the head and neck in humans. The 5-years survival rate of patients with LSCC have declined in the past four decades. microRNAs (miRNAs) has been reported to be capable of predicting the prognosis outcomes of patients with different cancers. However, there are no reports on the usage of multi-miRNAs model as signature for the diagnosis or prognosis of LSCC. Methods: To establish the miRNAs expression-associated model for diagnosis, prognosis prediction and aided therapy of patients with LSCC, the present study enrolled 107 patients with LSCC in clinic and obtained 117 LSCC samples data from TCGA database for evaluation, respectively. Next generation sequencing (NGS), raw data processing, the least absolute shrinkage and selection operator algorithm, Cox regression analysis, construction of nomogram and cell function assays (including proliferation, migration and invasion assays) were sequentially performed. Results: There were massively dysregulated miRNAs in the LSCC compared to normal tissues. A six-miRNAs signature consists of miR-137–3p, miR-3934–5p, miR-1276, miR-129–5p, miR-7-5p and miR-105–5p was built for prognosis prediction of LSCC patients. The six-miRNAs signature is strongly associated with the poor overall survival (OS, p = 2.5e-05, HR: 4.30 [2.20–8.50]), progression free interval (PFI, p = 0.025, HR: 1.94 [1.08–3.46]) and disease specific survival (DSS, p = 1.1e-05, HR: 5.00 [2.50–10.00]). A nomogram for prediction of 2-, 3- and 5-years OS was also developed based on the six-miRNAs signature and clinical features. Furthermore, blocking the function of each of the six miRNAs inhibited proliferation, invasion and migration of LSCC cells. Conclusions: The performance of six-miRNAs signature described in the current study demonstrated remarkable potential for progression assessment of LSCC. Moreover, the six-miRNAs signature may serve as predictive tool for prognosis and therapeutic targets of LSCC in clinic.http://www.sciencedirect.com/science/article/pii/S2468054023000483MicroRNAMolecular biomarkersOtorhinolaryngology Head and Neck SurgeryLaryngeal squamous cell carcinomaPrognosis predictionOverall survival |
| spellingShingle | Linshi Zhang Zhe Zhang Xiwang Zheng Yan Lu Li Dai Wenqi Li Hui Liu Shuxin Wen Qiuping Xie Xiangmin Zhang Ping Wang Yongyan Wu Wei Gao A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma Non-coding RNA Research MicroRNA Molecular biomarkers Otorhinolaryngology Head and Neck Surgery Laryngeal squamous cell carcinoma Prognosis prediction Overall survival |
| title | A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma |
| title_full | A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma |
| title_fullStr | A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma |
| title_full_unstemmed | A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma |
| title_short | A novel microRNA panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma |
| title_sort | novel microrna panel exhibited significant potential in evaluating the progression of laryngeal squamous cell carcinoma |
| topic | MicroRNA Molecular biomarkers Otorhinolaryngology Head and Neck Surgery Laryngeal squamous cell carcinoma Prognosis prediction Overall survival |
| url | http://www.sciencedirect.com/science/article/pii/S2468054023000483 |
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