<i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization
Modern broiler chickens have ongoing bone health problems. Phosphorus (P) plays an important role in bone development and increased understanding of P metabolism should improve the skeletal health of broilers. <i>Enterococcus faecium</i> has been widely used as a probiotic in broiler pro...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/7/1232 |
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| author | Weiwei Wang Huiyi Cai Anrong Zhang Zhimin Chen Wenhuan Chang Guohua Liu Xuejuan Deng Wayne L. Bryden Aijuan Zheng |
| author_facet | Weiwei Wang Huiyi Cai Anrong Zhang Zhimin Chen Wenhuan Chang Guohua Liu Xuejuan Deng Wayne L. Bryden Aijuan Zheng |
| author_sort | Weiwei Wang |
| collection | DOAJ |
| description | Modern broiler chickens have ongoing bone health problems. Phosphorus (P) plays an important role in bone development and increased understanding of P metabolism should improve the skeletal health of broilers. <i>Enterococcus faecium</i> has been widely used as a probiotic in broiler production and is shown to improve skeletal health of rats, but its effect on the bones of broilers remains unclear. This study investigated the effect of <i>E. faecium</i> on P absorption and utilization in broilers and the associated changes in the gut microbiota using 16S rDNA sequencing. Dietary supplementation with <i>E. faecium</i> improved P absorption through upregulation of the expression of intestinal <i>NaP-IIb</i> mRNA and increased the concentration of serum alkaline phosphatase. These actions increased P retention and bone mineralization in <i>E. faecium</i>-treated broilers. The positive effects of <i>E. faecium</i> on P metabolism were associated with changes in the populations of the intestinal microbiota. There was increased relative abundance of the following genera, <i>Alistipes</i>, <i>Eubacterium</i>, <i>Rikenella</i> and <i>Ruminococcaceae</i> and a decrease in the relative abundance of <i>Faecalibacterium</i> and <i>Escherichia-Shigella</i>. Dietary supplementation with <i>E. faecium</i> changed gut microbiota populations of broilers, increased the relative abundance of SCFA (short-chain fatty acid)-producing bacteria, improved intestinal P absorption and bone forming metabolic activities, and decreased P excretion. <i>E. faecium</i> facilitates increased utilisation of P in broilers. |
| first_indexed | 2024-03-10T18:20:34Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T18:20:34Z |
| publishDate | 2020-07-01 |
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| series | Animals |
| spelling | doaj.art-22cc37d32b884409ad99bfe69f85961b2023-11-20T07:19:33ZengMDPI AGAnimals2076-26152020-07-01107123210.3390/ani10071232<i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and UtilizationWeiwei Wang0Huiyi Cai1Anrong Zhang2Zhimin Chen3Wenhuan Chang4Guohua Liu5Xuejuan Deng6Wayne L. Bryden7Aijuan Zheng8Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaNational Engineering Research Center of Biological Feed Development, Beijing 100081, ChinaSchool of Agriculture and Food Sciences, University of Queensland, Gatton, QLD 4343, AustraliaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaModern broiler chickens have ongoing bone health problems. Phosphorus (P) plays an important role in bone development and increased understanding of P metabolism should improve the skeletal health of broilers. <i>Enterococcus faecium</i> has been widely used as a probiotic in broiler production and is shown to improve skeletal health of rats, but its effect on the bones of broilers remains unclear. This study investigated the effect of <i>E. faecium</i> on P absorption and utilization in broilers and the associated changes in the gut microbiota using 16S rDNA sequencing. Dietary supplementation with <i>E. faecium</i> improved P absorption through upregulation of the expression of intestinal <i>NaP-IIb</i> mRNA and increased the concentration of serum alkaline phosphatase. These actions increased P retention and bone mineralization in <i>E. faecium</i>-treated broilers. The positive effects of <i>E. faecium</i> on P metabolism were associated with changes in the populations of the intestinal microbiota. There was increased relative abundance of the following genera, <i>Alistipes</i>, <i>Eubacterium</i>, <i>Rikenella</i> and <i>Ruminococcaceae</i> and a decrease in the relative abundance of <i>Faecalibacterium</i> and <i>Escherichia-Shigella</i>. Dietary supplementation with <i>E. faecium</i> changed gut microbiota populations of broilers, increased the relative abundance of SCFA (short-chain fatty acid)-producing bacteria, improved intestinal P absorption and bone forming metabolic activities, and decreased P excretion. <i>E. faecium</i> facilitates increased utilisation of P in broilers.https://www.mdpi.com/2076-2615/10/7/1232broilerphosphorus<i>Enterococcus faecium</i>microbiota16S rDNA |
| spellingShingle | Weiwei Wang Huiyi Cai Anrong Zhang Zhimin Chen Wenhuan Chang Guohua Liu Xuejuan Deng Wayne L. Bryden Aijuan Zheng <i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization Animals broiler phosphorus <i>Enterococcus faecium</i> microbiota 16S rDNA |
| title | <i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization |
| title_full | <i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization |
| title_fullStr | <i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization |
| title_full_unstemmed | <i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization |
| title_short | <i>Enterococcus faecium</i> Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization |
| title_sort | i enterococcus faecium i modulates the gut microbiota of broilers and enhances phosphorus absorption and utilization |
| topic | broiler phosphorus <i>Enterococcus faecium</i> microbiota 16S rDNA |
| url | https://www.mdpi.com/2076-2615/10/7/1232 |
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