Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker
Muscovite mica with an amino silane-modified surface is commonly used as a substrate in atomic force microscopy (AFM) studies of biological macromolecules. Herein, the efficiency of two different protein immobilization strategies employing either (N-hydroxysuccinimide ester)-based crosslinker (DSP)...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Minerals |
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| Online Access: | https://www.mdpi.com/2075-163X/10/5/464 |
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| author | Anastasia A. Valueva Ivan D. Shumov Anna L. Kaysheva Irina A. Ivanova Vadim S. Ziborov Yuri D. Ivanov Tatyana O. Pleshakova |
| author_facet | Anastasia A. Valueva Ivan D. Shumov Anna L. Kaysheva Irina A. Ivanova Vadim S. Ziborov Yuri D. Ivanov Tatyana O. Pleshakova |
| author_sort | Anastasia A. Valueva |
| collection | DOAJ |
| description | Muscovite mica with an amino silane-modified surface is commonly used as a substrate in atomic force microscopy (AFM) studies of biological macromolecules. Herein, the efficiency of two different protein immobilization strategies employing either (N-hydroxysuccinimide ester)-based crosslinker (DSP) or benzophenone-based photoactivatable crosslinker (SuccBB) has been compared using AFM and mass spectrometry analysis. Two proteins with different physicochemical properties—human serum albumin (HSA) and horseradish peroxidase enzyme protein (HRP)—have been used as model objects in the study. In the case of HRP, both crosslinkers exhibited high immobilization efficiency—as opposed to the case with HSA, when sufficient capturing efficiency has only been observed with SuccBB photocrosslinker. The results obtained herein can find their application in commonly employed bioanalytical systems and in the development of novel highly sensitive chip-based diagnostic platforms employing immobilized proteins. The obtained data can also be of interest for other research areas in medicine and biotechnology employing immobilized biomolecules. |
| first_indexed | 2024-03-10T19:42:31Z |
| format | Article |
| id | doaj.art-22cdcea8fe3145459faaafb6b60e8b8f |
| institution | Directory Open Access Journal |
| issn | 2075-163X |
| language | English |
| last_indexed | 2024-03-10T19:42:31Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Minerals |
| spelling | doaj.art-22cdcea8fe3145459faaafb6b60e8b8f2023-11-20T01:04:53ZengMDPI AGMinerals2075-163X2020-05-0110546410.3390/min10050464Covalent Protein Immobilization onto Muscovite Mica Surface with a PhotocrosslinkerAnastasia A. Valueva0Ivan D. Shumov1Anna L. Kaysheva2Irina A. Ivanova3Vadim S. Ziborov4Yuri D. Ivanov5Tatyana O. Pleshakova6Institute of Biomedical Chemistry, Pogodinskaya st., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, 119121 Moscow, RussiaMuscovite mica with an amino silane-modified surface is commonly used as a substrate in atomic force microscopy (AFM) studies of biological macromolecules. Herein, the efficiency of two different protein immobilization strategies employing either (N-hydroxysuccinimide ester)-based crosslinker (DSP) or benzophenone-based photoactivatable crosslinker (SuccBB) has been compared using AFM and mass spectrometry analysis. Two proteins with different physicochemical properties—human serum albumin (HSA) and horseradish peroxidase enzyme protein (HRP)—have been used as model objects in the study. In the case of HRP, both crosslinkers exhibited high immobilization efficiency—as opposed to the case with HSA, when sufficient capturing efficiency has only been observed with SuccBB photocrosslinker. The results obtained herein can find their application in commonly employed bioanalytical systems and in the development of novel highly sensitive chip-based diagnostic platforms employing immobilized proteins. The obtained data can also be of interest for other research areas in medicine and biotechnology employing immobilized biomolecules.https://www.mdpi.com/2075-163X/10/5/464muscovite micaatomic force microscopycrosslinkerprotein immobilization |
| spellingShingle | Anastasia A. Valueva Ivan D. Shumov Anna L. Kaysheva Irina A. Ivanova Vadim S. Ziborov Yuri D. Ivanov Tatyana O. Pleshakova Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker Minerals muscovite mica atomic force microscopy crosslinker protein immobilization |
| title | Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker |
| title_full | Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker |
| title_fullStr | Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker |
| title_full_unstemmed | Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker |
| title_short | Covalent Protein Immobilization onto Muscovite Mica Surface with a Photocrosslinker |
| title_sort | covalent protein immobilization onto muscovite mica surface with a photocrosslinker |
| topic | muscovite mica atomic force microscopy crosslinker protein immobilization |
| url | https://www.mdpi.com/2075-163X/10/5/464 |
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