GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs.
Non-translating RNAs that have undergone active translational repression are culled from the cytoplasm into P-bodies for decapping-dependent decay or for sequestration. Organisms that use microRNA-mediated RNA silencing have an additional pathway to remove RNAs from active translation. Consequently,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4773245?pdf=render |
| _version_ | 1818987227947991040 |
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| author | Prajal H Patel Scott A Barbee J Todd Blankenship |
| author_facet | Prajal H Patel Scott A Barbee J Todd Blankenship |
| author_sort | Prajal H Patel |
| collection | DOAJ |
| description | Non-translating RNAs that have undergone active translational repression are culled from the cytoplasm into P-bodies for decapping-dependent decay or for sequestration. Organisms that use microRNA-mediated RNA silencing have an additional pathway to remove RNAs from active translation. Consequently, proteins that govern microRNA-mediated silencing, such as GW182/Gw and AGO1, are often associated with the P-bodies of higher eukaryotic organisms. Due to the presence of Gw, these structures have been referred to as GW-bodies. However, several reports have indicated that GW-bodies have different dynamics to P-bodies. Here, we use live imaging to examine GW-body and P-body dynamics in the early Drosophila melanogaster embryo. While P-bodies are present throughout early embryonic development, cytoplasmic GW-bodies only form in significant numbers at the midblastula transition. Unlike P-bodies, which are predominantly cytoplasmic, GW-bodies are present in both nuclei and the cytoplasm. RNA decapping factors such as DCP1, Me31B, and Hpat are not associated with GW-bodies, indicating that P-bodies and GW-bodies are distinct structures. Furthermore, known Gw interactors such as AGO1 and the CCR4-NOT deadenylation complex, which have been shown to be important for Gw function, are also not present in GW-bodies. Use of translational inhibitors puromycin and cycloheximide, which respectively increase or decrease cellular pools of non-translating RNAs, alter GW-body size, underscoring that GW-bodies are composed of non-translating RNAs. Taken together, these data indicate that active translational silencing most likely does not occur in GW-bodies. Instead GW-bodies most likely function as repositories for translationally silenced RNAs. Finally, inhibition of zygotic gene transcription is unable to block the formation of either P-bodies or GW-bodies in the early embryo, suggesting that these structures are composed of maternal RNAs. |
| first_indexed | 2024-12-20T19:03:21Z |
| format | Article |
| id | doaj.art-22ce259860a74e37ad2b0a34ade62dae |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T19:03:21Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-22ce259860a74e37ad2b0a34ade62dae2022-12-21T19:29:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015029110.1371/journal.pone.0150291GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs.Prajal H PatelScott A BarbeeJ Todd BlankenshipNon-translating RNAs that have undergone active translational repression are culled from the cytoplasm into P-bodies for decapping-dependent decay or for sequestration. Organisms that use microRNA-mediated RNA silencing have an additional pathway to remove RNAs from active translation. Consequently, proteins that govern microRNA-mediated silencing, such as GW182/Gw and AGO1, are often associated with the P-bodies of higher eukaryotic organisms. Due to the presence of Gw, these structures have been referred to as GW-bodies. However, several reports have indicated that GW-bodies have different dynamics to P-bodies. Here, we use live imaging to examine GW-body and P-body dynamics in the early Drosophila melanogaster embryo. While P-bodies are present throughout early embryonic development, cytoplasmic GW-bodies only form in significant numbers at the midblastula transition. Unlike P-bodies, which are predominantly cytoplasmic, GW-bodies are present in both nuclei and the cytoplasm. RNA decapping factors such as DCP1, Me31B, and Hpat are not associated with GW-bodies, indicating that P-bodies and GW-bodies are distinct structures. Furthermore, known Gw interactors such as AGO1 and the CCR4-NOT deadenylation complex, which have been shown to be important for Gw function, are also not present in GW-bodies. Use of translational inhibitors puromycin and cycloheximide, which respectively increase or decrease cellular pools of non-translating RNAs, alter GW-body size, underscoring that GW-bodies are composed of non-translating RNAs. Taken together, these data indicate that active translational silencing most likely does not occur in GW-bodies. Instead GW-bodies most likely function as repositories for translationally silenced RNAs. Finally, inhibition of zygotic gene transcription is unable to block the formation of either P-bodies or GW-bodies in the early embryo, suggesting that these structures are composed of maternal RNAs.http://europepmc.org/articles/PMC4773245?pdf=render |
| spellingShingle | Prajal H Patel Scott A Barbee J Todd Blankenship GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs. PLoS ONE |
| title | GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs. |
| title_full | GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs. |
| title_fullStr | GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs. |
| title_full_unstemmed | GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs. |
| title_short | GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs. |
| title_sort | gw bodies and p bodies constitute two separate pools of sequestered non translating rnas |
| url | http://europepmc.org/articles/PMC4773245?pdf=render |
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