Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron

Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron

Abstract Background Ordered transposon-insertion collections, in which specific transposon-insertion mutants are stored as monocultures in a genome-scale collection, represent a promising tool for genetic dissection of human gut microbiota members. However, publicly available collections are scarce...

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Bibliographic Details
Main Authors: Heidi A. Arjes, Jiawei Sun, Hualan Liu, Taylor H. Nguyen, Rebecca N. Culver, Arianna I. Celis, Sophie Jean Walton, Kimberly S. Vasquez, Feiqiao Brian Yu, Katherine S. Xue, Daniel Newton, Ricardo Zermeno, Meredith Weglarz, Adam Deutschbauer, Kerwyn Casey Huang, Anthony L. Shiver
Format: Article
Language:English
Published: BMC 2022-12-01
Series:BMC Biology
Subjects:
B. theta
Transposon mutagenesis
Ordered library
BarSeq
RB-TnSeq
Microbiome
Online Access:https://doi.org/10.1186/s12915-022-01481-2

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