Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i>
<i>Yarrowia lipolytica</i>, a GRAS (generally recognized as safe) nonconventional yeast, has been used widely in industrial fermentation to produce chemicals, fuels, and functional sugars such as erythritol and mannitol. Although <i>Y. lipolytica</i> is a promising organism f...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/6/509 |
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| author | Mengchan Liu Hairong Cheng |
| author_facet | Mengchan Liu Hairong Cheng |
| author_sort | Mengchan Liu |
| collection | DOAJ |
| description | <i>Yarrowia lipolytica</i>, a GRAS (generally recognized as safe) nonconventional yeast, has been used widely in industrial fermentation to produce chemicals, fuels, and functional sugars such as erythritol and mannitol. Although <i>Y. lipolytica</i> is a promising organism for bioconversion and has substantial potential in industrial production, its utilization is restricted by the high cost of cooling during the fermentation process; the optimum growth or fermentation temperature of <i>Y. lipolytica</i> is 28–30 °C, which is lower than that of some fermenting species. Therefore, it is necessary to breed a thermoresistant <i>Y. lipolytica</i> for use in a fermentation system. Here, we report a new thermoduric <i>Y. lipolytica</i> strain (a thermoresistant clone, HRC) that can grow at 35 °C—higher than the starting strain <i>Y. lipolytica</i> CGMCC7326 (maximum growth temperature at 33 °C)—by laboratory adaptive evolution. Based on the transcriptome analysis of the mutant strain HRC and the parental strain <i>Y. lipolytica</i> CGMCC7326 at different temperatures, 22 genes with increased expression at high temperatures were identified and 10 of them were overexpressed in <i>Y. lipolytica</i> CGMCC7326. HRC1, HRC2, and HRC3 (with YALI0B21582g, YALI0C13750g, and YALI0B10626g overexpressed, respectively) were assessed for growth at higher temperatures. This revealed that these three genes were related to thermotolerance. This study provides insights into the metabolic landscape of <i>Y. lipolytica</i> under heat stress, enabling future metabolic engineering endeavors to improve both thermoresistance and sugar alcohol production in the yeast <i>Y. lipolytica</i>. |
| first_indexed | 2024-03-11T02:29:29Z |
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| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T02:29:29Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-22d33ac99d4c42779ebf0976d87349bd2023-11-18T10:20:44ZengMDPI AGFermentation2311-56372023-05-019650910.3390/fermentation9060509Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i>Mengchan Liu0Hairong Cheng1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China<i>Yarrowia lipolytica</i>, a GRAS (generally recognized as safe) nonconventional yeast, has been used widely in industrial fermentation to produce chemicals, fuels, and functional sugars such as erythritol and mannitol. Although <i>Y. lipolytica</i> is a promising organism for bioconversion and has substantial potential in industrial production, its utilization is restricted by the high cost of cooling during the fermentation process; the optimum growth or fermentation temperature of <i>Y. lipolytica</i> is 28–30 °C, which is lower than that of some fermenting species. Therefore, it is necessary to breed a thermoresistant <i>Y. lipolytica</i> for use in a fermentation system. Here, we report a new thermoduric <i>Y. lipolytica</i> strain (a thermoresistant clone, HRC) that can grow at 35 °C—higher than the starting strain <i>Y. lipolytica</i> CGMCC7326 (maximum growth temperature at 33 °C)—by laboratory adaptive evolution. Based on the transcriptome analysis of the mutant strain HRC and the parental strain <i>Y. lipolytica</i> CGMCC7326 at different temperatures, 22 genes with increased expression at high temperatures were identified and 10 of them were overexpressed in <i>Y. lipolytica</i> CGMCC7326. HRC1, HRC2, and HRC3 (with YALI0B21582g, YALI0C13750g, and YALI0B10626g overexpressed, respectively) were assessed for growth at higher temperatures. This revealed that these three genes were related to thermotolerance. This study provides insights into the metabolic landscape of <i>Y. lipolytica</i> under heat stress, enabling future metabolic engineering endeavors to improve both thermoresistance and sugar alcohol production in the yeast <i>Y. lipolytica</i>.https://www.mdpi.com/2311-5637/9/6/509<i>Yarrowia lipolytica</i>thermoresistancetranscriptome analysislaboratory evolution |
| spellingShingle | Mengchan Liu Hairong Cheng Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i> Fermentation <i>Yarrowia lipolytica</i> thermoresistance transcriptome analysis laboratory evolution |
| title | Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i> |
| title_full | Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i> |
| title_fullStr | Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i> |
| title_full_unstemmed | Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i> |
| title_short | Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast <i>Yarrowia lipolytica</i> |
| title_sort | discovery and functional evaluation of heat tolerance genes in the nonconventional yeast i yarrowia lipolytica i |
| topic | <i>Yarrowia lipolytica</i> thermoresistance transcriptome analysis laboratory evolution |
| url | https://www.mdpi.com/2311-5637/9/6/509 |
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