Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model
The probiotic Lacticaseibacillus rhamnosus strain HN001 has been shown to have several beneficial health effects for both pediatric and maternal groups, including reduced risk of eczema in infants and gestational diabetes and postnatal depression in mothers. While L. rhamnosus HN001 appears to modif...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1002369/full |
| _version_ | 1797989616020619264 |
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| author | Wayne Young Wayne Young Paul Maclean Kelly Dunstan Leigh Ryan Jason Peters Kelly Armstrong Rachel Anderson Hilary Dewhurst Melanie van Gendt Ryan N. Dilger James Dekker Neill Haggarty Nicole Roy Nicole Roy |
| author_facet | Wayne Young Wayne Young Paul Maclean Kelly Dunstan Leigh Ryan Jason Peters Kelly Armstrong Rachel Anderson Hilary Dewhurst Melanie van Gendt Ryan N. Dilger James Dekker Neill Haggarty Nicole Roy Nicole Roy |
| author_sort | Wayne Young |
| collection | DOAJ |
| description | The probiotic Lacticaseibacillus rhamnosus strain HN001 has been shown to have several beneficial health effects for both pediatric and maternal groups, including reduced risk of eczema in infants and gestational diabetes and postnatal depression in mothers. While L. rhamnosus HN001 appears to modify immune and gut barrier biomarkers, its mode of action remains to be fully elucidated. To gain insights into the role of HN001 on the infant microbiome, the impacts of L. rhamnosus HN001 supplementation was studied in 10-day old male piglets that were fed either infant formula, or infant formula with L. rhamnosus HN001 at a low (1.3 × 105 CFU/ml) or high dose (7.9 × 106 CFU/ml) daily for 24 days. The cecal and fecal microbial communities were assessed by shotgun metagenome sequencing and host gene expression in the cecum and colon tissue was assessed by RNA-seq. Piglet fecal samples showed only modest differences between controls and those receiving dietary L. rhamnosus HN001. However, striking differences between the three groups were observed for cecal samples. While total lactobacilli were significantly increased only in the high dose L. rhamnosus HN001 group, both high and low dose groups showed an up to twofold reduction across the Firmicutes phylum and up to fourfold increase in Prevotella compared to controls. Methanobrevibacter was also decreased in HN001 fed piglets. Microbial genes involved in carbohydrate and vitamin metabolism were among those that differed in relative abundance between those with and without L. rhamnosus HN001. Changes in the cecal microbiome were accompanied by increased expression of tight junction pathway genes and decreased autophagy pathway genes in the cecal tissue of piglets fed the higher dose of L. rhamnosus HN001. Our findings showed supplementation with L. rhamnosus HN001 caused substantial changes in the cecal microbiome with likely consequences for key microbial metabolic pathways. Host gene expression changes in the cecum support previous research showing L. rhamnosus HN001 beneficially impacts intestinal barrier function. We show that fecal samples may not adequately reflect microbiome composition higher in the gastrointestinal tract, with the implication that effects of probiotic consumption may be missed by examining only the fecal microbiome. |
| first_indexed | 2024-04-11T08:22:07Z |
| format | Article |
| id | doaj.art-22d732ce5b2f40c5b6f2c4be6bed1d75 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T08:22:07Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-22d732ce5b2f40c5b6f2c4be6bed1d752022-12-22T04:34:52ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-10-01910.3389/fnut.2022.10023691002369Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet modelWayne Young0Wayne Young1Paul Maclean2Kelly Dunstan3Leigh Ryan4Jason Peters5Kelly Armstrong6Rachel Anderson7Hilary Dewhurst8Melanie van Gendt9Ryan N. Dilger10James Dekker11Neill Haggarty12Nicole Roy13Nicole Roy14AgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandDepartment of Animal Sciences, University of Illinois, Urbana, IL, United StatesFonterra Research and Development Centre, Palmerston North, New ZealandFonterra Research and Development Centre, Palmerston North, New ZealandAgResearch, Te Ohu Rangahau Kai, Palmerston North, New ZealandRiddet Institute, Massey University, Palmerston North, New ZealandThe probiotic Lacticaseibacillus rhamnosus strain HN001 has been shown to have several beneficial health effects for both pediatric and maternal groups, including reduced risk of eczema in infants and gestational diabetes and postnatal depression in mothers. While L. rhamnosus HN001 appears to modify immune and gut barrier biomarkers, its mode of action remains to be fully elucidated. To gain insights into the role of HN001 on the infant microbiome, the impacts of L. rhamnosus HN001 supplementation was studied in 10-day old male piglets that were fed either infant formula, or infant formula with L. rhamnosus HN001 at a low (1.3 × 105 CFU/ml) or high dose (7.9 × 106 CFU/ml) daily for 24 days. The cecal and fecal microbial communities were assessed by shotgun metagenome sequencing and host gene expression in the cecum and colon tissue was assessed by RNA-seq. Piglet fecal samples showed only modest differences between controls and those receiving dietary L. rhamnosus HN001. However, striking differences between the three groups were observed for cecal samples. While total lactobacilli were significantly increased only in the high dose L. rhamnosus HN001 group, both high and low dose groups showed an up to twofold reduction across the Firmicutes phylum and up to fourfold increase in Prevotella compared to controls. Methanobrevibacter was also decreased in HN001 fed piglets. Microbial genes involved in carbohydrate and vitamin metabolism were among those that differed in relative abundance between those with and without L. rhamnosus HN001. Changes in the cecal microbiome were accompanied by increased expression of tight junction pathway genes and decreased autophagy pathway genes in the cecal tissue of piglets fed the higher dose of L. rhamnosus HN001. Our findings showed supplementation with L. rhamnosus HN001 caused substantial changes in the cecal microbiome with likely consequences for key microbial metabolic pathways. Host gene expression changes in the cecum support previous research showing L. rhamnosus HN001 beneficially impacts intestinal barrier function. We show that fecal samples may not adequately reflect microbiome composition higher in the gastrointestinal tract, with the implication that effects of probiotic consumption may be missed by examining only the fecal microbiome.https://www.frontiersin.org/articles/10.3389/fnut.2022.1002369/fullprobioticLacticaseibacillus rhamnosusHN001pigletmicrobiomegut |
| spellingShingle | Wayne Young Wayne Young Paul Maclean Kelly Dunstan Leigh Ryan Jason Peters Kelly Armstrong Rachel Anderson Hilary Dewhurst Melanie van Gendt Ryan N. Dilger James Dekker Neill Haggarty Nicole Roy Nicole Roy Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model Frontiers in Nutrition probiotic Lacticaseibacillus rhamnosus HN001 piglet microbiome gut |
| title | Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model |
| title_full | Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model |
| title_fullStr | Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model |
| title_full_unstemmed | Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model |
| title_short | Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model |
| title_sort | lacticaseibacillus rhamnosus hn001 alters the microbiota composition in the cecum but not the feces in a piglet model |
| topic | probiotic Lacticaseibacillus rhamnosus HN001 piglet microbiome gut |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1002369/full |
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