Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in children
Abstract Circadian rhythms, which are governed by a circadian clock, regulate important biological processes associated with obesity. SNPs in circadian clock genes have been linked to energy and lipid homeostasis. The aim of our study was to evaluate the associations of CLOCK and REV-ERBα SNPs with...
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| Format: | Article |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49506-2 |
| _version_ | 1797388390468943872 |
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| author | Claudia Vales-Villamarín Olaya de Dios Ignacio Mahíllo-Fernández Macarena Perales Iris Pérez-Nadador Teresa Gavela-Pérez Leandro Soriano-Guillén Carmen Garcés |
| author_facet | Claudia Vales-Villamarín Olaya de Dios Ignacio Mahíllo-Fernández Macarena Perales Iris Pérez-Nadador Teresa Gavela-Pérez Leandro Soriano-Guillén Carmen Garcés |
| author_sort | Claudia Vales-Villamarín |
| collection | DOAJ |
| description | Abstract Circadian rhythms, which are governed by a circadian clock, regulate important biological processes associated with obesity. SNPs in circadian clock genes have been linked to energy and lipid homeostasis. The aim of our study was to evaluate the associations of CLOCK and REV-ERBα SNPs with BMI and plasma lipid levels in pre-pubertal boys and girls. The study sample population comprised 1268 children aged 6–8 years. Information regarding anthropometric parameters and plasma lipid concentrations was available. Genotyping of CLOCK SNPs rs1801260, rs4580704, rs3749474, rs3736544 and rs4864548 and REV-ERBα SNPs rs2017427, rs20711570 and rs2314339 was performed by RT-PCR. The CLOCK SNPs rs3749474 and rs4864548 were significantly associated with BMI in girls but no in boys. Female carriers of the minor alleles for these SNPs presented lower BMI compared to non-carriers. A significant association of the REV-ERBα SNP rs2071570 with plasma total cholesterol, LDL-cholesterol and Apo B in males was also observed. Male AA carriers showed lower plasma levels of total cholesterol, LDL-cholesterol and Apo B levels as compared with carriers of the C allele. No significant associations between any of the studied REV-ERBα SNPs and plasma lipid levels were observed in females. In summary, CLOCK and REV-ERBα SNPs were associated with BMI and plasma lipid levels respectively in a sex-dependent manner. Our findings suggest that sex-related factors may interact with Clock genes SNPs conditioning the effects of these polymorphisms on circadian alterations. |
| first_indexed | 2024-03-08T22:39:07Z |
| format | Article |
| id | doaj.art-22d9d1f001244e91b2b579bc0707994b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T22:39:07Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-22d9d1f001244e91b2b579bc0707994b2023-12-17T12:14:27ZengNature PortfolioScientific Reports2045-23222023-12-011311710.1038/s41598-023-49506-2Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in childrenClaudia Vales-Villamarín0Olaya de Dios1Ignacio Mahíllo-Fernández2Macarena Perales3Iris Pérez-Nadador4Teresa Gavela-Pérez5Leandro Soriano-Guillén6Carmen Garcés7Lipid Research Laboratory, IIS-Fundación Jiménez DíazLipid Research Laboratory, IIS-Fundación Jiménez DíazDepartment of Biostatistics and Epidemiology, IIS-Fundación Jiménez Díaz UAMLipid Research Laboratory, IIS-Fundación Jiménez DíazLipid Research Laboratory, IIS-Fundación Jiménez DíazDepartment of Pediatrics, IIS-Fundación Jiménez DíazDepartment of Pediatrics, IIS-Fundación Jiménez DíazLipid Research Laboratory, IIS-Fundación Jiménez DíazAbstract Circadian rhythms, which are governed by a circadian clock, regulate important biological processes associated with obesity. SNPs in circadian clock genes have been linked to energy and lipid homeostasis. The aim of our study was to evaluate the associations of CLOCK and REV-ERBα SNPs with BMI and plasma lipid levels in pre-pubertal boys and girls. The study sample population comprised 1268 children aged 6–8 years. Information regarding anthropometric parameters and plasma lipid concentrations was available. Genotyping of CLOCK SNPs rs1801260, rs4580704, rs3749474, rs3736544 and rs4864548 and REV-ERBα SNPs rs2017427, rs20711570 and rs2314339 was performed by RT-PCR. The CLOCK SNPs rs3749474 and rs4864548 were significantly associated with BMI in girls but no in boys. Female carriers of the minor alleles for these SNPs presented lower BMI compared to non-carriers. A significant association of the REV-ERBα SNP rs2071570 with plasma total cholesterol, LDL-cholesterol and Apo B in males was also observed. Male AA carriers showed lower plasma levels of total cholesterol, LDL-cholesterol and Apo B levels as compared with carriers of the C allele. No significant associations between any of the studied REV-ERBα SNPs and plasma lipid levels were observed in females. In summary, CLOCK and REV-ERBα SNPs were associated with BMI and plasma lipid levels respectively in a sex-dependent manner. Our findings suggest that sex-related factors may interact with Clock genes SNPs conditioning the effects of these polymorphisms on circadian alterations.https://doi.org/10.1038/s41598-023-49506-2 |
| spellingShingle | Claudia Vales-Villamarín Olaya de Dios Ignacio Mahíllo-Fernández Macarena Perales Iris Pérez-Nadador Teresa Gavela-Pérez Leandro Soriano-Guillén Carmen Garcés Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in children Scientific Reports |
| title | Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in children |
| title_full | Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in children |
| title_fullStr | Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in children |
| title_full_unstemmed | Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in children |
| title_short | Sex-dependent relationship of polymorphisms in CLOCK and REV-ERBα genes with body mass index and lipid levels in children |
| title_sort | sex dependent relationship of polymorphisms in clock and rev erbα genes with body mass index and lipid levels in children |
| url | https://doi.org/10.1038/s41598-023-49506-2 |
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