The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i>
The development of efficient bioprocesses requires inexpensive and renewable substrates. Molasses, a by-product of the sugar industry, contains mostly sucrose, a disaccharide composed of glucose and fructose, both easily absorbed by microorganisms. <i>Yarrowia lipolytica</i>, a platform...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/17/9282 |
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| author | Piotr Hapeta Patrycja Szczepańska Tadeusz Witkowski Jean-Marc Nicaud Anne-Marie Crutz-Le Coq Zbigniew Lazar |
| author_facet | Piotr Hapeta Patrycja Szczepańska Tadeusz Witkowski Jean-Marc Nicaud Anne-Marie Crutz-Le Coq Zbigniew Lazar |
| author_sort | Piotr Hapeta |
| collection | DOAJ |
| description | The development of efficient bioprocesses requires inexpensive and renewable substrates. Molasses, a by-product of the sugar industry, contains mostly sucrose, a disaccharide composed of glucose and fructose, both easily absorbed by microorganisms. <i>Yarrowia lipolytica</i>, a platform for the production of various chemicals, can be engineered for sucrose utilization by heterologous invertase expression, yet the problem of preferential use of glucose over fructose remains, as fructose consumption begins only after glucose depletion what significantly extends the bioprocesses. We investigated the role of hexose transporters and hexokinase (native and fructophilic) in this preference. Analysis of growth profiles and kinetics of monosaccharide utilization has proven that the glucose preference in <i>Y. lipolytica</i> depends primarily on the affinity of native hexokinase for glucose. Interestingly, combined overexpression of either hexokinase with hexose transporters significantly accelerated citric acid biosynthesis and enhanced pentose phosphate pathway leading to secretion of polyols (31.5 g/L vs. no polyols in the control strain). So far, polyol biosynthesis was efficient in glycerol-containing media. Moreover, overexpression of fructophilic hexokinase in combination with hexose transporters not only shortened this process to 48 h (84 h for the medium with glycerol) but also allowed to obtain 23% more polyols (40 g/L) compared to the glycerol medium (32.5 g/L). |
| first_indexed | 2024-03-10T08:11:26Z |
| format | Article |
| id | doaj.art-22de5bd7f6894e17950b340ce668825f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:11:26Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-22de5bd7f6894e17950b340ce668825f2023-11-22T10:41:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012217928210.3390/ijms22179282The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i>Piotr Hapeta0Patrycja Szczepańska1Tadeusz Witkowski2Jean-Marc Nicaud3Anne-Marie Crutz-Le Coq4Zbigniew Lazar5Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78352 Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78352 Jouy-en-Josas, FranceDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandThe development of efficient bioprocesses requires inexpensive and renewable substrates. Molasses, a by-product of the sugar industry, contains mostly sucrose, a disaccharide composed of glucose and fructose, both easily absorbed by microorganisms. <i>Yarrowia lipolytica</i>, a platform for the production of various chemicals, can be engineered for sucrose utilization by heterologous invertase expression, yet the problem of preferential use of glucose over fructose remains, as fructose consumption begins only after glucose depletion what significantly extends the bioprocesses. We investigated the role of hexose transporters and hexokinase (native and fructophilic) in this preference. Analysis of growth profiles and kinetics of monosaccharide utilization has proven that the glucose preference in <i>Y. lipolytica</i> depends primarily on the affinity of native hexokinase for glucose. Interestingly, combined overexpression of either hexokinase with hexose transporters significantly accelerated citric acid biosynthesis and enhanced pentose phosphate pathway leading to secretion of polyols (31.5 g/L vs. no polyols in the control strain). So far, polyol biosynthesis was efficient in glycerol-containing media. Moreover, overexpression of fructophilic hexokinase in combination with hexose transporters not only shortened this process to 48 h (84 h for the medium with glycerol) but also allowed to obtain 23% more polyols (40 g/L) compared to the glycerol medium (32.5 g/L).https://www.mdpi.com/1422-0067/22/17/9282<i>Yarrowia lipolytica</i>hexokinasehexose transportersglucosefructoseerythritol |
| spellingShingle | Piotr Hapeta Patrycja Szczepańska Tadeusz Witkowski Jean-Marc Nicaud Anne-Marie Crutz-Le Coq Zbigniew Lazar The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i> International Journal of Molecular Sciences <i>Yarrowia lipolytica</i> hexokinase hexose transporters glucose fructose erythritol |
| title | The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i> |
| title_full | The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i> |
| title_fullStr | The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i> |
| title_full_unstemmed | The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i> |
| title_short | The Role of Hexokinase and Hexose Transporters in Preferential Use of Glucose over Fructose and Downstream Metabolic Pathways in the Yeast <i>Yarrowia lipolytica</i> |
| title_sort | role of hexokinase and hexose transporters in preferential use of glucose over fructose and downstream metabolic pathways in the yeast i yarrowia lipolytica i |
| topic | <i>Yarrowia lipolytica</i> hexokinase hexose transporters glucose fructose erythritol |
| url | https://www.mdpi.com/1422-0067/22/17/9282 |
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