Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children
Abstract Objective To characterize the Staphylococcus aureus strains colonizing healthy Spanish children. Methods Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonizatio...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-10-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.1235 |
| _version_ | 1818984910496464896 |
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| author | Federico Román Ana Mendez‐Echevarria Teresa Del Rosal Cesar Garcia‐Vera Luis Escosa‐Garcia Martin Agud Fernando Chaves José Gutiérrez‐Fernández Enrique Ruiz de Gopegui Guillermo Ruiz‐Carrascoso Maria del Carmen Ruiz‐Gallego Albert Bernet Sara Maria Quevedo Ana Maria Fernández‐Verdugo Talia Sainz Cristina Calvo |
| author_facet | Federico Román Ana Mendez‐Echevarria Teresa Del Rosal Cesar Garcia‐Vera Luis Escosa‐Garcia Martin Agud Fernando Chaves José Gutiérrez‐Fernández Enrique Ruiz de Gopegui Guillermo Ruiz‐Carrascoso Maria del Carmen Ruiz‐Gallego Albert Bernet Sara Maria Quevedo Ana Maria Fernández‐Verdugo Talia Sainz Cristina Calvo |
| author_sort | Federico Román |
| collection | DOAJ |
| description | Abstract Objective To characterize the Staphylococcus aureus strains colonizing healthy Spanish children. Methods Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonization of the anterior nostrils was analyzed. MecA and mecC genes, antibiotic susceptibility, and genotyping according to the spa were determined in all strains, and the following toxins were examined: Panton‐Valentine leucocidin (pvl), toxic shock syndrome toxin (tst), and exfoliative toxins (eta, etb, etd). Multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCCmec) typing were performed on methicillin‐resistant Staphylococcus aureus (MRSA) strains, as well as pulsed‐field gel electrophoresis (PFGE). Results 619 strains were isolated in 1876 children (33%), and 92% of them were sent for characterization to the Spanish National Centre of Microbiology (n = 572). Twenty (3.5%) of these strains were mecA‐positive. Several spa types were detected among MRSA, being t002 the most frequently observed (30%), associating with SCCmec IVc. Among MSSA, 33% were positive for tst, while only 0.73% were positive for pvl. The 20 MRSA strains were negative for pvl, and 6 (30%) harbored the tst gene. Conclusions methicillin‐resistant Staphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% of S. aureus strains were positive for tst. |
| first_indexed | 2024-12-20T18:26:31Z |
| format | Article |
| id | doaj.art-22deb8c7429c487b9146fdecb4feb06b |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-20T18:26:31Z |
| publishDate | 2021-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-22deb8c7429c487b9146fdecb4feb06b2022-12-21T19:30:07ZengWileyMicrobiologyOpen2045-88272021-10-01105n/an/a10.1002/mbo3.1235Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish childrenFederico Román0Ana Mendez‐Echevarria1Teresa Del Rosal2Cesar Garcia‐Vera3Luis Escosa‐Garcia4Martin Agud5Fernando Chaves6José Gutiérrez‐Fernández7Enrique Ruiz de Gopegui8Guillermo Ruiz‐Carrascoso9Maria del Carmen Ruiz‐Gallego10Albert Bernet11Sara Maria Quevedo12Ana Maria Fernández‐Verdugo13Talia Sainz14Cristina Calvo15Nosocomial Infections Unit CNM Carlos III Health Institute Madrid SpainPaediatric Infectious and Tropical Diseases Department La Paz University Hospital and Translational Research Network in Paediatric Infectious Diseases (RITIP) Institute for Health Research IdiPAZ Madrid SpainPaediatric Infectious and Tropical Diseases Department La Paz University Hospital and Translational Research Network in Paediatric Infectious Diseases (RITIP) Institute for Health Research IdiPAZ Madrid SpainPrimary Healthcare Centre "José Ramón Muñoz Fernández" Aragón Health Service Zaragoza SpainPaediatric Infectious and Tropical Diseases Department La Paz University Hospital and Translational Research Network in Paediatric Infectious Diseases (RITIP) Institute for Health Research IdiPAZ Madrid SpainPaediatric Infectious and Tropical Diseases Department La Paz University Hospital Madrid SpainDepartment of Clinical Microbiology University Hospital 12 de Octubre Madrid SpainDepartment of Microbiology Hospital Virgen de las Nieves Institute for Biosanitary Research‐Ibs Granada SpainDepartment of Clinical Microbiology University Hospital Son Espases Palma SpainDepartment of Clinical Microbiology La Paz University Hospital Madrid SpainDepartment of Microbiology University Hospital Virgen del Rocío Sevilla SpainSection of Microbiology Arnau de Vilanova University Hospital Lleida SpainDepartment of Microbiology University Hospital Severo Ochoa Leganes SpainDepartment of Microbiology Central University Hospital of Asturias Oviedo SpainPaediatric Infectious and Tropical Diseases Department La Paz University Hospital and Translational Research Network in Paediatric Infectious Diseases (RITIP) Institute for Health Research IdiPAZ Madrid SpainPaediatric Infectious and Tropical Diseases Department La Paz University Hospital and Translational Research Network in Paediatric Infectious Diseases (RITIP) Institute for Health Research IdiPAZ Madrid SpainAbstract Objective To characterize the Staphylococcus aureus strains colonizing healthy Spanish children. Methods Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonization of the anterior nostrils was analyzed. MecA and mecC genes, antibiotic susceptibility, and genotyping according to the spa were determined in all strains, and the following toxins were examined: Panton‐Valentine leucocidin (pvl), toxic shock syndrome toxin (tst), and exfoliative toxins (eta, etb, etd). Multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCCmec) typing were performed on methicillin‐resistant Staphylococcus aureus (MRSA) strains, as well as pulsed‐field gel electrophoresis (PFGE). Results 619 strains were isolated in 1876 children (33%), and 92% of them were sent for characterization to the Spanish National Centre of Microbiology (n = 572). Twenty (3.5%) of these strains were mecA‐positive. Several spa types were detected among MRSA, being t002 the most frequently observed (30%), associating with SCCmec IVc. Among MSSA, 33% were positive for tst, while only 0.73% were positive for pvl. The 20 MRSA strains were negative for pvl, and 6 (30%) harbored the tst gene. Conclusions methicillin‐resistant Staphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% of S. aureus strains were positive for tst.https://doi.org/10.1002/mbo3.1235methicillin‐resistantmolecular characterizationnasal colonizationStaphylococcus aureus |
| spellingShingle | Federico Román Ana Mendez‐Echevarria Teresa Del Rosal Cesar Garcia‐Vera Luis Escosa‐Garcia Martin Agud Fernando Chaves José Gutiérrez‐Fernández Enrique Ruiz de Gopegui Guillermo Ruiz‐Carrascoso Maria del Carmen Ruiz‐Gallego Albert Bernet Sara Maria Quevedo Ana Maria Fernández‐Verdugo Talia Sainz Cristina Calvo Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children MicrobiologyOpen methicillin‐resistant molecular characterization nasal colonization Staphylococcus aureus |
| title | Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children |
| title_full | Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children |
| title_fullStr | Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children |
| title_full_unstemmed | Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children |
| title_short | Characterization of methicillin‐resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children |
| title_sort | characterization of methicillin resistant staphylococcus aureus strains colonizing the nostrils of spanish children |
| topic | methicillin‐resistant molecular characterization nasal colonization Staphylococcus aureus |
| url | https://doi.org/10.1002/mbo3.1235 |
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