miR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX)
Ovarian cancer is the leading cause of death from gynecologic cancer, reflecting its chemoresistance and frequent late diagnosis, and suggesting that a more effective treatment approach is needed. Lysyl oxidase (LOX) is involved in important biological processes such as gene regulation, cel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600020W.pdf |
| _version_ | 1811322275094331392 |
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| author | Wang Xuan Wang Yan Wang Guichan Liu Peishu |
| author_facet | Wang Xuan Wang Yan Wang Guichan Liu Peishu |
| author_sort | Wang Xuan |
| collection | DOAJ |
| description | Ovarian cancer is the leading cause of death from gynecologic cancer,
reflecting its chemoresistance and frequent late diagnosis, and suggesting
that a more effective treatment approach is needed. Lysyl oxidase (LOX) is
involved in important biological processes such as gene regulation, cell
signaling and cell motility, its deregulation contributing to tumor
formation and development. Although it is known that LOX is involved in
proliferation, migration and invasion in several types of tumors, studies of
LOX in ovarian cancers are scarce. To explore the molecular regulation
mechanisms in ovarian cancer tumorigenesis, the expression change
and the function of LOX was confirmed in ovarian tissues and cells, which
suggested that LOX is a tumor suppressor gene. To further understand how LOX
expression is regulated in ovarian cancer, microRNAs(miRNAs) were considered
because of their role in post-transcriptional regulation of many genes.
Recent work has described differential expression of mature miRNAs in human
cancers. Bioinformatics prediction which was used to find the appropriate
miRNA regulating LOX, revealed that miR-29b regulates LOX protein level via
its binding site on the 3'UTR of LOX mRNAin ES-2 cells, a human ovarian
clear cell carcinoma cell line. miR-29b knockdown inhibited proliferation and
invasion in ES-2 cells. Taken together, these findings suggest that
influencing LOX regulation bychanging the level of miR-29b expression could
provide a novel potential approachfor treating human ovarian clear cell
carcinoma. |
| first_indexed | 2024-04-13T13:33:31Z |
| format | Article |
| id | doaj.art-22e1089be739440892c0b87109e41799 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-04-13T13:33:31Z |
| publishDate | 2016-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-22e1089be739440892c0b87109e417992022-12-22T02:44:52ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392016-01-0168115516310.2298/ABS150420020W0354-46641600020WmiR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX)Wang Xuan0Wang Yan1Wang Guichan2Liu Peishu3Shandong University, Qilu Hospital, Department of Gynecology, Jinan City, Shandong Province, P.R. China + Qingdao University School of Medicine, Yantai Yuhuangding Hospital, Department of Gynecology, Yantai City, Shandong Province, P.R. ChinaQingdao University School of MedicineYantai Yuhuangding Hospital, , Department of Gynecology, Yantai City, Shandong Province, P.R. ChinaQingdao University School of Medicine, Yantai Yuhuangding Hospital, Department of Obstetrics, Yantai City, Shandong Province, P.R. ChinaShandong University, Qilu Hospital, Department of Gynecology, Jinan City, Shandong Province, P.R. ChinaOvarian cancer is the leading cause of death from gynecologic cancer, reflecting its chemoresistance and frequent late diagnosis, and suggesting that a more effective treatment approach is needed. Lysyl oxidase (LOX) is involved in important biological processes such as gene regulation, cell signaling and cell motility, its deregulation contributing to tumor formation and development. Although it is known that LOX is involved in proliferation, migration and invasion in several types of tumors, studies of LOX in ovarian cancers are scarce. To explore the molecular regulation mechanisms in ovarian cancer tumorigenesis, the expression change and the function of LOX was confirmed in ovarian tissues and cells, which suggested that LOX is a tumor suppressor gene. To further understand how LOX expression is regulated in ovarian cancer, microRNAs(miRNAs) were considered because of their role in post-transcriptional regulation of many genes. Recent work has described differential expression of mature miRNAs in human cancers. Bioinformatics prediction which was used to find the appropriate miRNA regulating LOX, revealed that miR-29b regulates LOX protein level via its binding site on the 3'UTR of LOX mRNAin ES-2 cells, a human ovarian clear cell carcinoma cell line. miR-29b knockdown inhibited proliferation and invasion in ES-2 cells. Taken together, these findings suggest that influencing LOX regulation bychanging the level of miR-29b expression could provide a novel potential approachfor treating human ovarian clear cell carcinoma.http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600020W.pdfOvarian cancerLOXmiR-29bproliferation and invasionhuman ovarian clear cell carcinoma |
| spellingShingle | Wang Xuan Wang Yan Wang Guichan Liu Peishu miR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX) Archives of Biological Sciences Ovarian cancer LOX miR-29b proliferation and invasion human ovarian clear cell carcinoma |
| title | miR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX) |
| title_full | miR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX) |
| title_fullStr | miR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX) |
| title_full_unstemmed | miR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX) |
| title_short | miR-29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting Lysyl oxidase (LOX) |
| title_sort | mir 29b regulates cell proliferation and invasion in human ovarian clear cell carcinoma by targeting lysyl oxidase lox |
| topic | Ovarian cancer LOX miR-29b proliferation and invasion human ovarian clear cell carcinoma |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600020W.pdf |
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