Immunohistochemical Differentiation of Triple Negative Breast Cancer
Based on immunohistochemical staining for the basal markers cytokeratin 5/6 (CK 5/6), cytokeratin 14 (CK 14) and P-cadherin, triple negative tumors (TNT) are divided into two groups: 1) basal-like (BL) positive for one or all three markers; and 2) non basal-like (NBL) negative for all three markers...
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| Format: | Article |
| Language: | English |
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Sestre Milosrdnice University hospital, Institute of Clinical Medical Research
2016-01-01
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| Series: | Acta Clinica Croatica |
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| Online Access: | https://hrcak.srce.hr/file/237717 |
| _version_ | 1827283133733535744 |
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| author | Miroslav Lesar Mladen Stanec Nikola Lesar Danko Velimir Vrdoljak Zvonimir Zore Marija Banović Gordana Brozović |
| author_facet | Miroslav Lesar Mladen Stanec Nikola Lesar Danko Velimir Vrdoljak Zvonimir Zore Marija Banović Gordana Brozović |
| author_sort | Miroslav Lesar |
| collection | DOAJ |
| description | Based on immunohistochemical staining for the basal markers cytokeratin 5/6 (CK 5/6), cytokeratin 14 (CK 14) and P-cadherin, triple negative tumors (TNT) are divided into two groups: 1) basal-like (BL) positive for one or all three markers; and 2) non basal-like (NBL)
negative for all three markers. Even though the different origin of the cells of these two types of tumors implies different biological properties, they had been treated as one entity until recently. This
paper analyzes TNT collected from 150 patients and distributed into two groups according to the results of immunohistochemical analysis, i.e. BL 116 (77.3%) and NBL 34 (22.67%). In this study, CK 5/6, CK 14 and P-cadherin were used as markers for identifying BL tumors. The immunohistochemical reaction was positive for CK 5/6 in 37%, for CK 14 in 50.86% and for P-cadherin in 68.34% of cases. The subclassification of triple negative breast cancer using the basal markers CK 5/6, CK 14 and P-cadherin has enabled identification of BL and NBL breast cancers in a proportion that is in line with the only accurate analysis of TNT gene expression. Using the mentioned combination of markers in daily practice is easy to perform and economically affordable. |
| first_indexed | 2024-04-24T09:30:40Z |
| format | Article |
| id | doaj.art-22e5495fb96a4177b42906ff8733941a |
| institution | Directory Open Access Journal |
| issn | 0353-9466 1333-9451 |
| language | English |
| last_indexed | 2024-04-24T09:30:40Z |
| publishDate | 2016-01-01 |
| publisher | Sestre Milosrdnice University hospital, Institute of Clinical Medical Research |
| record_format | Article |
| series | Acta Clinica Croatica |
| spelling | doaj.art-22e5495fb96a4177b42906ff8733941a2024-04-15T13:53:26ZengSestre Milosrdnice University hospital, Institute of Clinical Medical ResearchActa Clinica Croatica0353-94661333-94512016-01-0155.1.3810.20471/acc.2016.55.01.1Immunohistochemical Differentiation of Triple Negative Breast CancerMiroslav Lesar0Mladen Stanec1Nikola Lesar2Danko Velimir Vrdoljak3Zvonimir Zore4Marija Banović5Gordana Brozović6Department of Oncology Surgery,University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaDepartment of Oncology and Plastic Surgery, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaDepartment of Anesthesiology,University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zaqgreb, CroatiaDepartment of Oncology Surgery, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, CroatiaDepartment of Oncology Surgery,University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia,student, School of Medicine, University of Zagreb, Zagreb, CroatiaDepartment of Anesthesiology, University Hospital for Tumors, Sestre milosrdnice University Hospital Center, Zagreb, Croatia; Faculty of Medicine, University of Osijek, Osijek, CroatiaBased on immunohistochemical staining for the basal markers cytokeratin 5/6 (CK 5/6), cytokeratin 14 (CK 14) and P-cadherin, triple negative tumors (TNT) are divided into two groups: 1) basal-like (BL) positive for one or all three markers; and 2) non basal-like (NBL) negative for all three markers. Even though the different origin of the cells of these two types of tumors implies different biological properties, they had been treated as one entity until recently. This paper analyzes TNT collected from 150 patients and distributed into two groups according to the results of immunohistochemical analysis, i.e. BL 116 (77.3%) and NBL 34 (22.67%). In this study, CK 5/6, CK 14 and P-cadherin were used as markers for identifying BL tumors. The immunohistochemical reaction was positive for CK 5/6 in 37%, for CK 14 in 50.86% and for P-cadherin in 68.34% of cases. The subclassification of triple negative breast cancer using the basal markers CK 5/6, CK 14 and P-cadherin has enabled identification of BL and NBL breast cancers in a proportion that is in line with the only accurate analysis of TNT gene expression. Using the mentioned combination of markers in daily practice is easy to perform and economically affordable.https://hrcak.srce.hr/file/237717Carcinoma, ductal, breastTriple negative breast neoplasmsBiomarkers, tumorBasallike tumorsNon basal-like tumorsAdult |
| spellingShingle | Miroslav Lesar Mladen Stanec Nikola Lesar Danko Velimir Vrdoljak Zvonimir Zore Marija Banović Gordana Brozović Immunohistochemical Differentiation of Triple Negative Breast Cancer Acta Clinica Croatica Carcinoma, ductal, breast Triple negative breast neoplasms Biomarkers, tumor Basallike tumors Non basal-like tumors Adult |
| title | Immunohistochemical Differentiation of Triple Negative Breast Cancer |
| title_full | Immunohistochemical Differentiation of Triple Negative Breast Cancer |
| title_fullStr | Immunohistochemical Differentiation of Triple Negative Breast Cancer |
| title_full_unstemmed | Immunohistochemical Differentiation of Triple Negative Breast Cancer |
| title_short | Immunohistochemical Differentiation of Triple Negative Breast Cancer |
| title_sort | immunohistochemical differentiation of triple negative breast cancer |
| topic | Carcinoma, ductal, breast Triple negative breast neoplasms Biomarkers, tumor Basallike tumors Non basal-like tumors Adult |
| url | https://hrcak.srce.hr/file/237717 |
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