Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo
In order to better understand differences in the outcome of infectious bursal disease virus (IBDV) infection, we inoculated a very virulent (vv) strain into White Leghorn chickens of inbred line W that was previously reported to experience over 24% flock mortality, and three inbred lines (15I, C.B4...
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MDPI AG
2021-05-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/5/933 |
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| author | Amin S. Asfor Salik Nazki Vishwanatha R.A.P. Reddy Elle Campbell Katherine L. Dulwich Efstathios S. Giotis Michael A. Skinner Andrew J. Broadbent |
| author_facet | Amin S. Asfor Salik Nazki Vishwanatha R.A.P. Reddy Elle Campbell Katherine L. Dulwich Efstathios S. Giotis Michael A. Skinner Andrew J. Broadbent |
| author_sort | Amin S. Asfor |
| collection | DOAJ |
| description | In order to better understand differences in the outcome of infectious bursal disease virus (IBDV) infection, we inoculated a very virulent (vv) strain into White Leghorn chickens of inbred line W that was previously reported to experience over 24% flock mortality, and three inbred lines (15I, C.B4 and 0) that were previously reported to display no mortality. Within each experimental group, some individuals experienced more severe disease than others but line 15I birds experienced milder disease based on average clinical scores, percentage of birds with gross pathology, average bursal lesion scores and average peak bursal virus titre. RNA-Seq analysis revealed that more severe disease in line W was associated with significant up-regulation of pathways involved in inflammation, cytoskeletal regulation by Rho GTPases, nicotinic acetylcholine receptor signaling, and Wnt signaling in the bursa compared to line 15I. Primary bursal cell populations isolated from uninfected line W birds contained a significantly greater percentage of KUL01+ macrophages than cells isolated from line 15I birds (<i>p</i> < 0.01) and, when stimulated ex vivo with LPS, showed more rapid up-regulation of pro-inflammatory gene expression than those from line 15I birds. We hypothesize that a more rapid induction of pro-inflammatory cytokine responses in bursal cells following IBDV infection leads to more severe disease in line W birds than in line 15I. |
| first_indexed | 2024-03-10T11:17:49Z |
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| id | doaj.art-22e5f37bcd7445498634323e93e1d0f3 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T11:17:49Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-22e5f37bcd7445498634323e93e1d0f32023-11-21T20:13:57ZengMDPI AGViruses1999-49152021-05-0113593310.3390/v13050933Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex VivoAmin S. Asfor0Salik Nazki1Vishwanatha R.A.P. Reddy2Elle Campbell3Katherine L. Dulwich4Efstathios S. Giotis5Michael A. Skinner6Andrew J. Broadbent7Birnaviruses Group, The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKBirnaviruses Group, The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKBirnaviruses Group, The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKBirnaviruses Group, The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKBirnaviruses Group, The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKSection of Virology, Faculty of Medicine, Imperial College London, St. Mary’s Campus, Norfolk Place, London W2 1PG, UKSection of Virology, Faculty of Medicine, Imperial College London, St. Mary’s Campus, Norfolk Place, London W2 1PG, UKBirnaviruses Group, The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UKIn order to better understand differences in the outcome of infectious bursal disease virus (IBDV) infection, we inoculated a very virulent (vv) strain into White Leghorn chickens of inbred line W that was previously reported to experience over 24% flock mortality, and three inbred lines (15I, C.B4 and 0) that were previously reported to display no mortality. Within each experimental group, some individuals experienced more severe disease than others but line 15I birds experienced milder disease based on average clinical scores, percentage of birds with gross pathology, average bursal lesion scores and average peak bursal virus titre. RNA-Seq analysis revealed that more severe disease in line W was associated with significant up-regulation of pathways involved in inflammation, cytoskeletal regulation by Rho GTPases, nicotinic acetylcholine receptor signaling, and Wnt signaling in the bursa compared to line 15I. Primary bursal cell populations isolated from uninfected line W birds contained a significantly greater percentage of KUL01+ macrophages than cells isolated from line 15I birds (<i>p</i> < 0.01) and, when stimulated ex vivo with LPS, showed more rapid up-regulation of pro-inflammatory gene expression than those from line 15I birds. We hypothesize that a more rapid induction of pro-inflammatory cytokine responses in bursal cells following IBDV infection leads to more severe disease in line W birds than in line 15I.https://www.mdpi.com/1999-4915/13/5/933IBDVinfectious bursal diseasebursa of Fabriciusinbred lineschickens |
| spellingShingle | Amin S. Asfor Salik Nazki Vishwanatha R.A.P. Reddy Elle Campbell Katherine L. Dulwich Efstathios S. Giotis Michael A. Skinner Andrew J. Broadbent Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo Viruses IBDV infectious bursal disease bursa of Fabricius inbred lines chickens |
| title | Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo |
| title_full | Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo |
| title_fullStr | Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo |
| title_full_unstemmed | Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo |
| title_short | Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo |
| title_sort | transcriptomic analysis of inbred chicken lines reveals infectious bursal disease severity is associated with greater bursal inflammation in vivo and more rapid induction of pro inflammatory responses in primary bursal cells stimulated ex vivo |
| topic | IBDV infectious bursal disease bursa of Fabricius inbred lines chickens |
| url | https://www.mdpi.com/1999-4915/13/5/933 |
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