Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorum
Abstract Background The objective of this study was to evaluate the effects of glutamine on the growth performance and systemic innate immune response in broiler chickens challenged with Salmonella pullorum. A total of 600 one-day-old Arbor Acres broiler chickens were assigned randomly to 6 dietary...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-023-03836-5 |
| _version_ | 1797388249355780096 |
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| author | Qiu jue Wu Long long Zhu Rong kai Zhang Zhong ying Xing Cong Wang Jia hui Liao Nai zhi Hu Bin yao Cheng Yan Ma Yu qin Wang |
| author_facet | Qiu jue Wu Long long Zhu Rong kai Zhang Zhong ying Xing Cong Wang Jia hui Liao Nai zhi Hu Bin yao Cheng Yan Ma Yu qin Wang |
| author_sort | Qiu jue Wu |
| collection | DOAJ |
| description | Abstract Background The objective of this study was to evaluate the effects of glutamine on the growth performance and systemic innate immune response in broiler chickens challenged with Salmonella pullorum. A total of 600 one-day-old Arbor Acres broiler chickens were assigned randomly to 6 dietary treatments with 10 replicates for a 21-day feeding experiment. The experimental treatments were as follows: the control treatment (birds fed the basal diet), the Gln1 treatment, and the Gln 2 treatment (birds fed the basal diet supplemented with 0.5%, and 1.0% Glutamine, respectively). At 3 d of age, half of the birds from each treatment were challenged oral gavage with 2.0 × 104 CFU/mL of S. pullorum suspension (1.0 mL per bird) or an equivalent amount of sterile saline alone, which served as a control. Results The results showed that S. pullorum infection had adverse effects on the average daily feed intake, average daily gain, and feed conversion ratio of broiler chickens compared with those of the CON treatment on d 7, decreased the spleen and bursa of fabricius relative weights (except on d 21), serum immunoglobulin A (IgA),immunoglobulin G (IgG), and immunoglobulin M (IgM) concentrations, and spleen melanoma differentiation-associated gene 5 (MDA5) and laboratory of genetics and physiology gene 2 (LGP2) mRNA expression levels, and increased the mRNA expression levels of spleen Nodinitib-1 (NOD1), Toll-like receptors 2,4 (TLR2, TLR4), DNA-dependent activator of IFN-regulatory factors (DAI), mitochondrial antiviral-signaling protein (MAVS), P50, P65, and RelB on d 4, 7, 14, and 21. Supplementation with Gln improved the relative weights of the spleen and bursa of Fabricius (except on d 21), increased the serum IgA, IgG, and IgM concentrations and the mRNA expression levels of spleen MDA5 and LGP2, and decreased the mRNA expression levels of spleen NOD1, TLR2, TLR4, DAI, MAVS, P50, P65, and RelB of S. pullorum-challenged broiler chickens. Conclusion These results indicate that Gln might stimulate the systemic innate immune responses of the spleen in broiler chickens challenged with S. pullorum. |
| first_indexed | 2024-03-08T22:38:04Z |
| format | Article |
| id | doaj.art-22e8599b9f974ac7a1e438db17272289 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-08T22:38:04Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-22e8599b9f974ac7a1e438db172722892023-12-17T12:20:34ZengBMCBMC Veterinary Research1746-61482023-12-0119111110.1186/s12917-023-03836-5Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorumQiu jue Wu0Long long Zhu1Rong kai Zhang2Zhong ying Xing3Cong Wang4Jia hui Liao5Nai zhi Hu6Bin yao Cheng7Yan Ma8Yu qin Wang9College of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyCollege of Animal Science and Technology, Henan University of Science and TechnologyAbstract Background The objective of this study was to evaluate the effects of glutamine on the growth performance and systemic innate immune response in broiler chickens challenged with Salmonella pullorum. A total of 600 one-day-old Arbor Acres broiler chickens were assigned randomly to 6 dietary treatments with 10 replicates for a 21-day feeding experiment. The experimental treatments were as follows: the control treatment (birds fed the basal diet), the Gln1 treatment, and the Gln 2 treatment (birds fed the basal diet supplemented with 0.5%, and 1.0% Glutamine, respectively). At 3 d of age, half of the birds from each treatment were challenged oral gavage with 2.0 × 104 CFU/mL of S. pullorum suspension (1.0 mL per bird) or an equivalent amount of sterile saline alone, which served as a control. Results The results showed that S. pullorum infection had adverse effects on the average daily feed intake, average daily gain, and feed conversion ratio of broiler chickens compared with those of the CON treatment on d 7, decreased the spleen and bursa of fabricius relative weights (except on d 21), serum immunoglobulin A (IgA),immunoglobulin G (IgG), and immunoglobulin M (IgM) concentrations, and spleen melanoma differentiation-associated gene 5 (MDA5) and laboratory of genetics and physiology gene 2 (LGP2) mRNA expression levels, and increased the mRNA expression levels of spleen Nodinitib-1 (NOD1), Toll-like receptors 2,4 (TLR2, TLR4), DNA-dependent activator of IFN-regulatory factors (DAI), mitochondrial antiviral-signaling protein (MAVS), P50, P65, and RelB on d 4, 7, 14, and 21. Supplementation with Gln improved the relative weights of the spleen and bursa of Fabricius (except on d 21), increased the serum IgA, IgG, and IgM concentrations and the mRNA expression levels of spleen MDA5 and LGP2, and decreased the mRNA expression levels of spleen NOD1, TLR2, TLR4, DAI, MAVS, P50, P65, and RelB of S. pullorum-challenged broiler chickens. Conclusion These results indicate that Gln might stimulate the systemic innate immune responses of the spleen in broiler chickens challenged with S. pullorum.https://doi.org/10.1186/s12917-023-03836-5GlutamineInnate immune responseSalmonella pullorumSpleenBroiler chickens |
| spellingShingle | Qiu jue Wu Long long Zhu Rong kai Zhang Zhong ying Xing Cong Wang Jia hui Liao Nai zhi Hu Bin yao Cheng Yan Ma Yu qin Wang Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorum BMC Veterinary Research Glutamine Innate immune response Salmonella pullorum Spleen Broiler chickens |
| title | Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorum |
| title_full | Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorum |
| title_fullStr | Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorum |
| title_full_unstemmed | Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorum |
| title_short | Effect of glutamine on the systemic innate immune response in broiler chickens challenged with Salmonella pullorum |
| title_sort | effect of glutamine on the systemic innate immune response in broiler chickens challenged with salmonella pullorum |
| topic | Glutamine Innate immune response Salmonella pullorum Spleen Broiler chickens |
| url | https://doi.org/10.1186/s12917-023-03836-5 |
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