Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species
Interspecies or even inter-genus somatic nuclear transfer is considered to be an effective means for conserving a wide variety of avian genetic resources. However, somatic nuclear transfer offspring production is currently limited to mammals. Therefore, the present experiment was designed in an at...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2008-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/45/2/45_45_143/_pdf/-char/en |
| _version_ | 1797816232789934080 |
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| author | Susumu Ishiguro Yukio Kanai Atsushi Tajima |
| author_facet | Susumu Ishiguro Yukio Kanai Atsushi Tajima |
| author_sort | Susumu Ishiguro |
| collection | DOAJ |
| description | Interspecies or even inter-genus somatic nuclear transfer is considered to be an effective means for conserving a wide variety of avian genetic resources. However, somatic nuclear transfer offspring production is currently limited to mammals. Therefore, the present experiment was designed in an attempt to produce inter-genus somatic nuclear transferred gonadal germ cells (snt-GGCs) between chicken and quail. Electrofusion was carried out between embryonic blood cells (EBCs) collected from 4-day-old embryos (donor cells) and gonadal germ cells (GGCs) from 7-day-old chick embryos or 6-day-old quail embryos (recipient cells). GGCs were labeled with PKH26 fluorescent dye as a marker. Electrofusion was carried out according to previously described methods. The combinations of donor-recipient cells were designed to contain all four possible combinations: E(c)-G(c), E(q)-G(q), E(q)-G(c) and E(c)-G(q), where E, G, c and q are abbreviations for EBCs, GGCs, chick and quail, respectively. Following electrofusion, the fusion solution containing cells were stained with 1μg/mL Hoechst 33342. PKH26-labeled cells with two or more nuclei of different sizes were determined to be snt-GGCs. The experiment was replicated ten times and snt-GGCs were observed in five (50%), three (30%), four (40%) and five (50%) replicates. The average number of snt-GGCs produced per replicate were 0.6 (1.2%), 0.3 (0.6%), 0.5 (1.0%) and 0.5 (1.0%) for E(c)-G(c), E(q)-G(q), E(q)-G(c) and E(c)-G(q), respectively. The present results demonstrate that inter-genus snt-GGCs can be produced by electrofusion using EBCs and GGCs in avian species. |
| first_indexed | 2024-03-13T08:34:41Z |
| format | Article |
| id | doaj.art-22ea7946e35e4784b8a94a0d035636e2 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:34:41Z |
| publishDate | 2008-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-22ea7946e35e4784b8a94a0d035636e22023-05-31T00:33:42ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862008-04-0145214314610.2141/jpsa.45.143jpsaProduction of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian SpeciesSusumu Ishiguro0Yukio Kanai1Atsushi Tajima2Graduate School of Life and Environmental Sciences, University of Tsukuba, JapanGraduate School of Life and Environmental Sciences, University of Tsukuba, JapanGraduate School of Life and Environmental Sciences, University of Tsukuba, JapanInterspecies or even inter-genus somatic nuclear transfer is considered to be an effective means for conserving a wide variety of avian genetic resources. However, somatic nuclear transfer offspring production is currently limited to mammals. Therefore, the present experiment was designed in an attempt to produce inter-genus somatic nuclear transferred gonadal germ cells (snt-GGCs) between chicken and quail. Electrofusion was carried out between embryonic blood cells (EBCs) collected from 4-day-old embryos (donor cells) and gonadal germ cells (GGCs) from 7-day-old chick embryos or 6-day-old quail embryos (recipient cells). GGCs were labeled with PKH26 fluorescent dye as a marker. Electrofusion was carried out according to previously described methods. The combinations of donor-recipient cells were designed to contain all four possible combinations: E(c)-G(c), E(q)-G(q), E(q)-G(c) and E(c)-G(q), where E, G, c and q are abbreviations for EBCs, GGCs, chick and quail, respectively. Following electrofusion, the fusion solution containing cells were stained with 1μg/mL Hoechst 33342. PKH26-labeled cells with two or more nuclei of different sizes were determined to be snt-GGCs. The experiment was replicated ten times and snt-GGCs were observed in five (50%), three (30%), four (40%) and five (50%) replicates. The average number of snt-GGCs produced per replicate were 0.6 (1.2%), 0.3 (0.6%), 0.5 (1.0%) and 0.5 (1.0%) for E(c)-G(c), E(q)-G(q), E(q)-G(c) and E(c)-G(q), respectively. The present results demonstrate that inter-genus snt-GGCs can be produced by electrofusion using EBCs and GGCs in avian species.https://www.jstage.jst.go.jp/article/jpsa/45/2/45_45_143/_pdf/-char/enchickenelectrofusiongonadal germ cellsquailsomatic nuclear transfer |
| spellingShingle | Susumu Ishiguro Yukio Kanai Atsushi Tajima Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species The Journal of Poultry Science chicken electrofusion gonadal germ cells quail somatic nuclear transfer |
| title | Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species |
| title_full | Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species |
| title_fullStr | Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species |
| title_full_unstemmed | Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species |
| title_short | Production of Inter-Genus Somatic Nuclear Transferred Gonadal Germ Cells (snt-GGCs) in Avian Species |
| title_sort | production of inter genus somatic nuclear transferred gonadal germ cells snt ggcs in avian species |
| topic | chicken electrofusion gonadal germ cells quail somatic nuclear transfer |
| url | https://www.jstage.jst.go.jp/article/jpsa/45/2/45_45_143/_pdf/-char/en |
| work_keys_str_mv | AT susumuishiguro productionofintergenussomaticnucleartransferredgonadalgermcellssntggcsinavianspecies AT yukiokanai productionofintergenussomaticnucleartransferredgonadalgermcellssntggcsinavianspecies AT atsushitajima productionofintergenussomaticnucleartransferredgonadalgermcellssntggcsinavianspecies |