Neuroprotective Effect of Dioscin on the Aging Brain
Our previous works have shown that dioscin, a natural product, has various pharmacological activities, however, its role in brain aging has not been reported. In the present study, in vitro H<sub>2</sub>O<sub>2</sub>-treated PC12 cells and in vivo <span style="font-va...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/7/1247 |
| _version_ | 1819017682639388672 |
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| author | Yan Qi Ruomiao Li Lina Xu Lianhong Yin Youwei Xu Xu Han Jinyong Peng |
| author_facet | Yan Qi Ruomiao Li Lina Xu Lianhong Yin Youwei Xu Xu Han Jinyong Peng |
| author_sort | Yan Qi |
| collection | DOAJ |
| description | Our previous works have shown that dioscin, a natural product, has various pharmacological activities, however, its role in brain aging has not been reported. In the present study, in vitro H<sub>2</sub>O<sub>2</sub>-treated PC12 cells and in vivo <span style="font-variant: small-caps;">d</span>-galactose-induced aging rat models were used to evaluate the neuroprotective effect of dioscin on brain aging. The results showed that dioscin increased cell viability and protected PC12 cells against oxidative stress through decreasing reactive oxygen species (ROS) and lactate dehydrogenase (LDH) levels. In vivo, dioscin markedly improved the spatial learning ability and memory of aging rats, reduced the protein carbonyl content and aging cell numbers, restored the levels of superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and nitric oxide synthase (NOS) in brain tissue, and reversed the histopathological structure changes of nerve cells. Mechanism studies showed that dioscin markedly adjusted the MAPK and Nrf2/ARE signalling pathways to decrease oxidative stress. Additionally, dioscin also significantly decreased inflammation by inhibiting the mRNA or protein levels of TNF-α, IL-1β, IL-6, CYP2E1 and HMGB1. Taken together, these results indicate that dioscin showed neuroprotective effect against brain aging via decreasing oxidative stress and inflammation, which should be developed as an efficient candidate in clinical to treat brain aging in the future. |
| first_indexed | 2024-12-21T03:07:25Z |
| format | Article |
| id | doaj.art-22ec67934152430fb0d569b32d19c623 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T03:07:25Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-22ec67934152430fb0d569b32d19c6232022-12-21T19:18:03ZengMDPI AGMolecules1420-30492019-03-01247124710.3390/molecules24071247molecules24071247Neuroprotective Effect of Dioscin on the Aging BrainYan Qi0Ruomiao Li1Lina Xu2Lianhong Yin3Youwei Xu4Xu Han5Jinyong Peng6College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, ChinaCollege of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, ChinaCollege of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, ChinaCollege of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, ChinaCollege of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, ChinaCollege of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, ChinaCollege of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, ChinaOur previous works have shown that dioscin, a natural product, has various pharmacological activities, however, its role in brain aging has not been reported. In the present study, in vitro H<sub>2</sub>O<sub>2</sub>-treated PC12 cells and in vivo <span style="font-variant: small-caps;">d</span>-galactose-induced aging rat models were used to evaluate the neuroprotective effect of dioscin on brain aging. The results showed that dioscin increased cell viability and protected PC12 cells against oxidative stress through decreasing reactive oxygen species (ROS) and lactate dehydrogenase (LDH) levels. In vivo, dioscin markedly improved the spatial learning ability and memory of aging rats, reduced the protein carbonyl content and aging cell numbers, restored the levels of superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and nitric oxide synthase (NOS) in brain tissue, and reversed the histopathological structure changes of nerve cells. Mechanism studies showed that dioscin markedly adjusted the MAPK and Nrf2/ARE signalling pathways to decrease oxidative stress. Additionally, dioscin also significantly decreased inflammation by inhibiting the mRNA or protein levels of TNF-α, IL-1β, IL-6, CYP2E1 and HMGB1. Taken together, these results indicate that dioscin showed neuroprotective effect against brain aging via decreasing oxidative stress and inflammation, which should be developed as an efficient candidate in clinical to treat brain aging in the future.https://www.mdpi.com/1420-3049/24/7/1247brain agingneuroprotectiondioscinoxidative stressinflammation |
| spellingShingle | Yan Qi Ruomiao Li Lina Xu Lianhong Yin Youwei Xu Xu Han Jinyong Peng Neuroprotective Effect of Dioscin on the Aging Brain Molecules brain aging neuroprotection dioscin oxidative stress inflammation |
| title | Neuroprotective Effect of Dioscin on the Aging Brain |
| title_full | Neuroprotective Effect of Dioscin on the Aging Brain |
| title_fullStr | Neuroprotective Effect of Dioscin on the Aging Brain |
| title_full_unstemmed | Neuroprotective Effect of Dioscin on the Aging Brain |
| title_short | Neuroprotective Effect of Dioscin on the Aging Brain |
| title_sort | neuroprotective effect of dioscin on the aging brain |
| topic | brain aging neuroprotection dioscin oxidative stress inflammation |
| url | https://www.mdpi.com/1420-3049/24/7/1247 |
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