The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease
Sugarcane Mosaic Virus (SCMV) infection is one of the most serious problems that can result in severe yield loss of sugarcane. Since the symptoms of SCMV infection are similar to other biotic and abiotic stress symptoms, the development of a rapid diagnostic with high precision is required. The use...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2023-03-01
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| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/74104 |
| _version_ | 1797852187429175296 |
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| author | Nurmalasari Darsono Widhi Dyah Sawitri Retnosari Apriasti Agus Heri Setyo Wahyudi Putri Andreyna Saragi Victorin Mega Putri Sugiharto Sugiharto Win Darmanto |
| author_facet | Nurmalasari Darsono Widhi Dyah Sawitri Retnosari Apriasti Agus Heri Setyo Wahyudi Putri Andreyna Saragi Victorin Mega Putri Sugiharto Sugiharto Win Darmanto |
| author_sort | Nurmalasari Darsono |
| collection | DOAJ |
| description | Sugarcane Mosaic Virus (SCMV) infection is one of the most serious problems that can result in severe yield loss of sugarcane. Since the symptoms of SCMV infection are similar to other biotic and abiotic stress symptoms, the development of a rapid diagnostic with high precision is required. The use of laboratory animals such as rabbits is required for antibody production in immunoassay‐based detection. However, due to its many advantages, specific chicken egg yolk immunoglobulin (IgY) has received considerable attention as an alternative antibody production in immunodiagnostics for infectious diseases. In this study, IgY antibody against SCMV recombinant coat protein (CP) was successfully obtained from chicken blood serum and tested to compare its efficacy against antibody from rabbit (IgG) using immunocapture reverse transcription‐polymerase chain reaction (IC‐RT‐PCR). The result showed that IgY and IgG could detect 0.1 g SCMV infected leaves using 1000‐times‐diluted antibodies. The IgY antibody was also confirmed to be reproducible and potentially applicable in plant disease diagnostics using an antibody‐based detection. |
| first_indexed | 2024-04-09T19:29:37Z |
| format | Article |
| id | doaj.art-22f07fd29ff746ce81bb5cb6d9576cdb |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-09T19:29:37Z |
| publishDate | 2023-03-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-22f07fd29ff746ce81bb5cb6d9576cdb2023-04-05T04:41:11ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412023-03-01281313610.22146/ijbiotech.7410433500The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus diseaseNurmalasari Darsono0Widhi Dyah Sawitri1Retnosari Apriasti2Agus Heri Setyo Wahyudi3Putri Andreyna Saragi4Victorin Mega Putri5Sugiharto Sugiharto6Win Darmanto7Biology Department, Faculty of Science and Technology, Airlangga University, Jalan Mulyorejo, Surabaya 60115, Indonesia; Research Center for Genetic Engineering, National Research and Innovation Agency, BJ Habibie Building, Jalan MH Thamrin 8, Jakarta Pusat 10340, IndonesiaAgronomy Department, Faculty of Agriculture, Universitas Gadjah Mada, Jalan Flora, Bulaksumur, Yogyakarta 55281, IndonesiaCenter for Development of Advanced Science and Technology (CDAST), University of Jember, Jalan Kalimantan 37, Jember 68121, IndonesiaBiotechnology Laboratory, PT Perkebunan Nusantara XI, Jl. Merak 1, Surabaya 60175, IndonesiaInstitute of Science Technology and Health, Jl. Kemuning 57A, Jombang 61413, IndonesiaInstitute of Science Technology and Health, Jl. Kemuning 57A, Jombang 61413, IndonesiaBiology Department, Faculty of Science and Technology, Airlangga University, Jalan Mulyorejo, Surabaya 60115, Indonesia; Institute of Science Technology and Health, Jl. Kemuning 57A, Jombang 61413, IndonesiaBiology Department, Faculty of Science and Technology, Airlangga University, Jalan Mulyorejo, Surabaya 60115, Indonesia; Institute of Science Technology and Health, Jl. Kemuning 57A, Jombang 61413, IndonesiaSugarcane Mosaic Virus (SCMV) infection is one of the most serious problems that can result in severe yield loss of sugarcane. Since the symptoms of SCMV infection are similar to other biotic and abiotic stress symptoms, the development of a rapid diagnostic with high precision is required. The use of laboratory animals such as rabbits is required for antibody production in immunoassay‐based detection. However, due to its many advantages, specific chicken egg yolk immunoglobulin (IgY) has received considerable attention as an alternative antibody production in immunodiagnostics for infectious diseases. In this study, IgY antibody against SCMV recombinant coat protein (CP) was successfully obtained from chicken blood serum and tested to compare its efficacy against antibody from rabbit (IgG) using immunocapture reverse transcription‐polymerase chain reaction (IC‐RT‐PCR). The result showed that IgY and IgG could detect 0.1 g SCMV infected leaves using 1000‐times‐diluted antibodies. The IgY antibody was also confirmed to be reproducible and potentially applicable in plant disease diagnostics using an antibody‐based detection.https://jurnal.ugm.ac.id/ijbiotech/article/view/74104igg antibodyigy antibodyimmunoassayimmunocapture reverse transcription‐polymerase chain reaction (ic‐rt‐ pcr)sugarcane mosaic virus (scmv) |
| spellingShingle | Nurmalasari Darsono Widhi Dyah Sawitri Retnosari Apriasti Agus Heri Setyo Wahyudi Putri Andreyna Saragi Victorin Mega Putri Sugiharto Sugiharto Win Darmanto The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease Indonesian Journal of Biotechnology igg antibody igy antibody immunoassay immunocapture reverse transcription‐polymerase chain reaction (ic‐rt‐ pcr) sugarcane mosaic virus (scmv) |
| title | The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease |
| title_full | The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease |
| title_fullStr | The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease |
| title_full_unstemmed | The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease |
| title_short | The efficacy of a chicken antibody for the development of immunoassay‐based rapid detection in sugarcane mosaic virus disease |
| title_sort | efficacy of a chicken antibody for the development of immunoassay based rapid detection in sugarcane mosaic virus disease |
| topic | igg antibody igy antibody immunoassay immunocapture reverse transcription‐polymerase chain reaction (ic‐rt‐ pcr) sugarcane mosaic virus (scmv) |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/74104 |
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