High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019
The Post-Ebola era (2017–2019) presented an opportunity for laboratory investments in Sierra Leone. US CDC supported the Ministry of Health and Sanitation to establish a microbiological unit for routine antimicrobial sensitivity testing in two referral (pediatric and maternity) hospitals in Freetown...
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MDPI AG
2021-06-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/6/2/103 |
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| author | Zikan Koroma Francis Moses Alexandre Delamou Katrina Hann Engy Ali Freddy Eric Kitutu Juliet Sanyu Namugambe Doris Harding Veerle Hermans Kudakwashe Takarinda Pruthu Thekkur Isatta Wurie |
| author_facet | Zikan Koroma Francis Moses Alexandre Delamou Katrina Hann Engy Ali Freddy Eric Kitutu Juliet Sanyu Namugambe Doris Harding Veerle Hermans Kudakwashe Takarinda Pruthu Thekkur Isatta Wurie |
| author_sort | Zikan Koroma |
| collection | DOAJ |
| description | The Post-Ebola era (2017–2019) presented an opportunity for laboratory investments in Sierra Leone. US CDC supported the Ministry of Health and Sanitation to establish a microbiological unit for routine antimicrobial sensitivity testing in two referral (pediatric and maternity) hospitals in Freetown. This study describes resistance patterns among patients’ laboratory samples from 2017 to 2019 using routine data. Samples included urine, stool, cerebrospinal fluid, pus-wound, pleural fluid, and high vaginal swabs. Selected Gram-positive and Gram-negative bacterial isolates were tested for antimicrobial susceptibility. Of 200 samples received by the laboratory, 89 returned positive bacterial isolates with urine and pus-wound swabs accounting for 75% of positive isolates. The number of positive isolates increased annually from one in 2017 to 42 in 2018 and 46 in 2019. Resistance of the cultures to at least one antibiotic was high (91%), and even higher in the pediatric hospital (94%). Resistance was highest with penicillin (81%) for Gram-positive bacteria and lowest with nitrofurantoin (13%). Gram-negative bacteria were most resistant to ampicillin, gentamycin, streptomycin, tetracycline, cephalothin and penicillin (100%) and least resistant to novobiocin (0%). Antibiotic resistance for commonly prescribed antibiotics was high in two referral hospitals, highlighting the urgent need for antimicrobial stewardship and access to reserve antibiotics. |
| first_indexed | 2024-03-10T10:22:11Z |
| format | Article |
| id | doaj.art-22f6019ed3c44e4583f1a94d97493022 |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-10T10:22:11Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-22f6019ed3c44e4583f1a94d974930222023-11-22T00:17:06ZengMDPI AGTropical Medicine and Infectious Disease2414-63662021-06-016210310.3390/tropicalmed6020103High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019Zikan Koroma0Francis Moses1Alexandre Delamou2Katrina Hann3Engy Ali4Freddy Eric Kitutu5Juliet Sanyu Namugambe6Doris Harding7Veerle Hermans8Kudakwashe Takarinda9Pruthu Thekkur10Isatta Wurie11Ministry of Health and Sanitation, Freetown P.O. Box 232, Sierra LeoneMinistry of Health and Sanitation, Freetown P.O. Box 232, Sierra LeoneMaferinyah Training and Research Centre, Forécariah 4099, GuineaSustainable Health Systems, Freetown P.O. Box 232, Sierra LeoneLuxembourg Operational Research Unit (LuxOR), Medical Department, Medecins Sans Frontieres—Operational Centre Brussels, L-1617 Luxembourg, LuxembourgStrengthening Pharmaceutical Systems (SPS), Department of Pharmacy, Makerere University School of Health Sciences, Kampala P.O. Box 7072, UgandaDepartment of Pharmacy, Mbarara University of Science and Technology (MUST), Mbarara P.O. Box 1410, UgandaMinistry of Health and Sanitation, Freetown P.O. Box 232, Sierra LeoneLuxembourg Operational Research Unit (LuxOR), Medical Department, Medecins Sans Frontieres—Operational Centre Brussels, L-1617 Luxembourg, LuxembourgCentre for Operational Research, International Union Against Tuberculosis & Lung Disease (The Union), 75006 Paris, FranceCentre for Operational Research, International Union Against Tuberculosis & Lung Disease (The Union), 75006 Paris, FranceDepartment of Chemical Pathology, College of Medicine and Allied Health Sciences, University of Sierra Leone, Freetown P.O. Box 232, Sierra LeoneThe Post-Ebola era (2017–2019) presented an opportunity for laboratory investments in Sierra Leone. US CDC supported the Ministry of Health and Sanitation to establish a microbiological unit for routine antimicrobial sensitivity testing in two referral (pediatric and maternity) hospitals in Freetown. This study describes resistance patterns among patients’ laboratory samples from 2017 to 2019 using routine data. Samples included urine, stool, cerebrospinal fluid, pus-wound, pleural fluid, and high vaginal swabs. Selected Gram-positive and Gram-negative bacterial isolates were tested for antimicrobial susceptibility. Of 200 samples received by the laboratory, 89 returned positive bacterial isolates with urine and pus-wound swabs accounting for 75% of positive isolates. The number of positive isolates increased annually from one in 2017 to 42 in 2018 and 46 in 2019. Resistance of the cultures to at least one antibiotic was high (91%), and even higher in the pediatric hospital (94%). Resistance was highest with penicillin (81%) for Gram-positive bacteria and lowest with nitrofurantoin (13%). Gram-negative bacteria were most resistant to ampicillin, gentamycin, streptomycin, tetracycline, cephalothin and penicillin (100%) and least resistant to novobiocin (0%). Antibiotic resistance for commonly prescribed antibiotics was high in two referral hospitals, highlighting the urgent need for antimicrobial stewardship and access to reserve antibiotics.https://www.mdpi.com/2414-6366/6/2/103AMRlaboratoryAMR surveillanceantimicrobial stewardshipoperational researchSORT IT |
| spellingShingle | Zikan Koroma Francis Moses Alexandre Delamou Katrina Hann Engy Ali Freddy Eric Kitutu Juliet Sanyu Namugambe Doris Harding Veerle Hermans Kudakwashe Takarinda Pruthu Thekkur Isatta Wurie High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019 Tropical Medicine and Infectious Disease AMR laboratory AMR surveillance antimicrobial stewardship operational research SORT IT |
| title | High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019 |
| title_full | High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019 |
| title_fullStr | High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019 |
| title_full_unstemmed | High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019 |
| title_short | High Levels of Antibiotic Resistance Patterns in Two Referral Hospitals during the Post-Ebola Era in Free-Town, Sierra Leone: 2017–2019 |
| title_sort | high levels of antibiotic resistance patterns in two referral hospitals during the post ebola era in free town sierra leone 2017 2019 |
| topic | AMR laboratory AMR surveillance antimicrobial stewardship operational research SORT IT |
| url | https://www.mdpi.com/2414-6366/6/2/103 |
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