In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2
Abstract The present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and mo...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-03-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-020-00303-z |
| _version_ | 1818901728464994304 |
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| author | Mahendra Kumar Sahu Komal Kaushik Amitava Das Harit Jha |
| author_facet | Mahendra Kumar Sahu Komal Kaushik Amitava Das Harit Jha |
| author_sort | Mahendra Kumar Sahu |
| collection | DOAJ |
| description | Abstract The present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and molecular techniques. Fungus was screened for the production of secondary metabolites using a specific medium. The metabolites were extracted using a suitable solvent and each fraction was subsequently evaluated for their antioxidant, antimicrobial, antiproliferative and anti-aging properties. The ethanolic extract depicted the highest antioxidant activity with 83%, 79%, 80% and 74% as assessed by ferric reducing power, 2,2-diphenyl 1-picrylhydrazyl, 2,2′-azino-bis3-ethylbenzthiazoline-6-sulfonic and phosphomolybdenum assays, respectively. Similarly, ethanolic extracts depicted marked antimicrobial activity as compared with standard antibiotics and antifungal agents as well as demonstrated significant antiproliferative property against a panel of mammalian cancer cell lines. Furthermore, different fractions of the purified ethanolic extract obtained using adsorption column chromatography were evaluated for antiproliferative property and identification of an active metabolite in the purified fraction using gas chromatography–mass spectroscopy and nuclear magnetic resonance techniques yielded 3-methyl-4-oxo-pentanoic acid. Thus, the present study suggests that the active metabolite 3-methyl-4-oxo-pentanoic acid extracted from Talaromyces purpureogenus isolate-ABRF2 has a potential antiproliferative, anti-aging, and antimicrobial therapeutic properties that will be further evaluated using in vivo studies in future. |
| first_indexed | 2024-12-19T20:24:22Z |
| format | Article |
| id | doaj.art-22f618b443b34b1c9faac2770d3f8539 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-19T20:24:22Z |
| publishDate | 2020-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-22f618b443b34b1c9faac2770d3f85392022-12-21T20:06:52ZengSpringerOpenBioresources and Bioprocessing2197-43652020-03-017111610.1186/s40643-020-00303-zIn vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2Mahendra Kumar Sahu0Komal Kaushik1Amitava Das2Harit Jha3Department of Biotechnology, Guru Ghasidas VishwavidyalayaDepartment of Applied Biology, CSIR-Indian Institute of Chemical TechnologyDepartment of Applied Biology, CSIR-Indian Institute of Chemical TechnologyDepartment of Biotechnology, Guru Ghasidas VishwavidyalayaAbstract The present study evaluated the potential biological activities of rhizospheric fungi isolated from the Achanakmar Biosphere Reserve, India. Fungus, Talaromyces purpureogenus isolate-ABRF2 from the soil of the Achanakmar biosphere was characterized by using morphological, biochemical and molecular techniques. Fungus was screened for the production of secondary metabolites using a specific medium. The metabolites were extracted using a suitable solvent and each fraction was subsequently evaluated for their antioxidant, antimicrobial, antiproliferative and anti-aging properties. The ethanolic extract depicted the highest antioxidant activity with 83%, 79%, 80% and 74% as assessed by ferric reducing power, 2,2-diphenyl 1-picrylhydrazyl, 2,2′-azino-bis3-ethylbenzthiazoline-6-sulfonic and phosphomolybdenum assays, respectively. Similarly, ethanolic extracts depicted marked antimicrobial activity as compared with standard antibiotics and antifungal agents as well as demonstrated significant antiproliferative property against a panel of mammalian cancer cell lines. Furthermore, different fractions of the purified ethanolic extract obtained using adsorption column chromatography were evaluated for antiproliferative property and identification of an active metabolite in the purified fraction using gas chromatography–mass spectroscopy and nuclear magnetic resonance techniques yielded 3-methyl-4-oxo-pentanoic acid. Thus, the present study suggests that the active metabolite 3-methyl-4-oxo-pentanoic acid extracted from Talaromyces purpureogenus isolate-ABRF2 has a potential antiproliferative, anti-aging, and antimicrobial therapeutic properties that will be further evaluated using in vivo studies in future.http://link.springer.com/article/10.1186/s40643-020-00303-zTalaromyces purpureogenusRhizosphericAntioxidantAntiproliferativeAnti-aging |
| spellingShingle | Mahendra Kumar Sahu Komal Kaushik Amitava Das Harit Jha In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2 Bioresources and Bioprocessing Talaromyces purpureogenus Rhizospheric Antioxidant Antiproliferative Anti-aging |
| title | In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2 |
| title_full | In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2 |
| title_fullStr | In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2 |
| title_full_unstemmed | In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2 |
| title_short | In vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus Talaromyces purpureogenus isolate-ABRF2 |
| title_sort | in vitro and in silico antioxidant and antiproliferative activity of rhizospheric fungus talaromyces purpureogenus isolate abrf2 |
| topic | Talaromyces purpureogenus Rhizospheric Antioxidant Antiproliferative Anti-aging |
| url | http://link.springer.com/article/10.1186/s40643-020-00303-z |
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