Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of Carotenoids
Carotenoids are fat-soluble bioactive compounds found in plants, animals, and microorganisms, which have a range of health benefits and great technological and industrial importance in the fields of pharmaceuticals, food, and biotechnology. Yeasts of the genus <i>Rhodotorula</i> can bios...
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MDPI AG
2023-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/7/617 |
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| author | Felipe de Andrade Maia Willen Silva Igreja Ana Augusta Odorissi Xavier Adriana Zerlotti Mercadante Alessandra Santos Lopes Renan Campos Chisté |
| author_facet | Felipe de Andrade Maia Willen Silva Igreja Ana Augusta Odorissi Xavier Adriana Zerlotti Mercadante Alessandra Santos Lopes Renan Campos Chisté |
| author_sort | Felipe de Andrade Maia |
| collection | DOAJ |
| description | Carotenoids are fat-soluble bioactive compounds found in plants, animals, and microorganisms, which have a range of health benefits and great technological and industrial importance in the fields of pharmaceuticals, food, and biotechnology. Yeasts of the genus <i>Rhodotorula</i> can biosynthesize large amounts of carotenoids under environmental stress conditions, and this process may become industrially feasible if production costs are minimized using low-cost by-products as nutrient sources. As cassava roots are produced and consumed in large quantities in the Amazon biome, our research aimed to investigate the best cultivation conditions in a benchtop bioreactor for the biotechnological production of carotenoids by <i>R. glutinis</i> using manipueira as a low-cost substrate. Manipueira is wastewater generated during cassava flour production, and it was concentrated at 12 °Brix for this study. In addition, the carotenoid composition, at the best cultivation condition, was analyzed by LC-MS. Higher carotenoid contents (1410 μg/g of dried biomass) than described in the literature, and biomass (10 g), were produced by <i>R. glutinis</i> using concentrated manipueira as a low-cost substrate after cultivation at pH 5, 35 °C and agitation at 150 rpm. At the best cultivation conditions, β-carotene, lutein, and 5,8-epoxy-lutein were the major carotenoids, with the latter two compounds being identified for the first time in the biomass of <i>R. glutinis</i>. Therefore, concentrated manipueira can be seen as a feasible alternative and low-cost substrate to be used for the biotechnological production of high carotenoid contents by <i>R. glutinis</i>. |
| first_indexed | 2024-03-11T01:05:25Z |
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| language | English |
| last_indexed | 2024-03-11T01:05:25Z |
| publishDate | 2023-06-01 |
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| series | Fermentation |
| spelling | doaj.art-22f64282f6784e88b5220db0cb6a60582023-11-18T19:15:57ZengMDPI AGFermentation2311-56372023-06-019761710.3390/fermentation9070617Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of CarotenoidsFelipe de Andrade Maia0Willen Silva Igreja1Ana Augusta Odorissi Xavier2Adriana Zerlotti Mercadante3Alessandra Santos Lopes4Renan Campos Chisté5Faculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Pará (UFPA), Belém 66075-110, BrazilPostgraduate Program of Food Science and Technology (PPGCTA), Institute of Technology (ITEC), Federal University of Pará (UFPA), Belém 66075-110, BrazilDepartment of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas (UNICAMP), São Paulo 13083-862, BrazilDepartment of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas (UNICAMP), São Paulo 13083-862, BrazilFaculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Pará (UFPA), Belém 66075-110, BrazilFaculty of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Pará (UFPA), Belém 66075-110, BrazilCarotenoids are fat-soluble bioactive compounds found in plants, animals, and microorganisms, which have a range of health benefits and great technological and industrial importance in the fields of pharmaceuticals, food, and biotechnology. Yeasts of the genus <i>Rhodotorula</i> can biosynthesize large amounts of carotenoids under environmental stress conditions, and this process may become industrially feasible if production costs are minimized using low-cost by-products as nutrient sources. As cassava roots are produced and consumed in large quantities in the Amazon biome, our research aimed to investigate the best cultivation conditions in a benchtop bioreactor for the biotechnological production of carotenoids by <i>R. glutinis</i> using manipueira as a low-cost substrate. Manipueira is wastewater generated during cassava flour production, and it was concentrated at 12 °Brix for this study. In addition, the carotenoid composition, at the best cultivation condition, was analyzed by LC-MS. Higher carotenoid contents (1410 μg/g of dried biomass) than described in the literature, and biomass (10 g), were produced by <i>R. glutinis</i> using concentrated manipueira as a low-cost substrate after cultivation at pH 5, 35 °C and agitation at 150 rpm. At the best cultivation conditions, β-carotene, lutein, and 5,8-epoxy-lutein were the major carotenoids, with the latter two compounds being identified for the first time in the biomass of <i>R. glutinis</i>. Therefore, concentrated manipueira can be seen as a feasible alternative and low-cost substrate to be used for the biotechnological production of high carotenoid contents by <i>R. glutinis</i>.https://www.mdpi.com/2311-5637/9/7/617<i>Rhodotorula</i>yeastbenchtop bioreactormanipueiraβ-carotene |
| spellingShingle | Felipe de Andrade Maia Willen Silva Igreja Ana Augusta Odorissi Xavier Adriana Zerlotti Mercadante Alessandra Santos Lopes Renan Campos Chisté Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of Carotenoids Fermentation <i>Rhodotorula</i> yeast benchtop bioreactor manipueira β-carotene |
| title | Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of Carotenoids |
| title_full | Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of Carotenoids |
| title_fullStr | Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of Carotenoids |
| title_full_unstemmed | Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of Carotenoids |
| title_short | Concentrated Manipueira as an Alternative Low-Cost Substrate to <i>Rhodotorula glutinis</i> for Biotechnological Production of High Contents of Carotenoids |
| title_sort | concentrated manipueira as an alternative low cost substrate to i rhodotorula glutinis i for biotechnological production of high contents of carotenoids |
| topic | <i>Rhodotorula</i> yeast benchtop bioreactor manipueira β-carotene |
| url | https://www.mdpi.com/2311-5637/9/7/617 |
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