Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea
Lipopolysaccharide (LPS) is a major component of outer-membrane gram-negative bacteria, and it can act as a Pathogen-Associated Molecular Pattern (PAMP) for perception of pathogens by plants. LPS can be recognized by plants, triggering certain plant defense-related responses, including callose depos...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2014-12-01
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| Series: | Makara Journal of Science |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/science/article/view/4343/3196 |
| _version_ | 1818345112656150528 |
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| author | Pipit Marianingsih Andi Salamah Yuki Ichinose |
| author_facet | Pipit Marianingsih Andi Salamah Yuki Ichinose |
| author_sort | Pipit Marianingsih |
| collection | DOAJ |
| description | Lipopolysaccharide (LPS) is a major component of outer-membrane gram-negative bacteria, and it can act as a Pathogen-Associated Molecular Pattern (PAMP) for perception of pathogens by plants. LPS can be recognized by plants, triggering certain plant defense-related responses, including callose deposition. This study investigated induction of callose deposition by bacterial LPS in tobacco. Tobacco leaves were infiltrated with 400 μg/mL and 800 μg/mL LPS extracted from Pseudomonas syringae pv. tabaci (Pta) and Pseudomonas syringae pv. glycinea (Pgl) and incubated for 24 h or 48 h. To detect callose deposition, tobacco leaves were cleared in lactophenol solution, stained with aniline blue, and visualized by fluorescence microscopy. Results showed that LPS from Pgl induced more callose deposition in tobacco leaves than did that from Pta. In addition, a Pearson correlation test revealed that incubation period was the most significant factor in callose deposition, followed by the type of LPS bacteria. However, LPS concentration was not significantly corelated to callose deposition in tobacco leaves. |
| first_indexed | 2024-12-13T16:57:12Z |
| format | Article |
| id | doaj.art-22f7e1c304044cec8353adc5e5e52841 |
| institution | Directory Open Access Journal |
| issn | 2339-1995 2356-0851 |
| language | English |
| last_indexed | 2024-12-13T16:57:12Z |
| publishDate | 2014-12-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Science |
| spelling | doaj.art-22f7e1c304044cec8353adc5e5e528412022-12-21T23:37:52ZengUniversitas IndonesiaMakara Journal of Science2339-19952356-08512014-12-0118412713210.7454/mss.v18i4.4343Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycineaPipit Marianingsih0Andi Salamah1Yuki Ichinose2Department of Biology Education, Faculty of Teacher Training and Education, Universitas Sultan Ageng Tirtayasa, Serang 42122, IndonesiaDepartment of Biology, Faculty of Mathematic and Natural Sciences, Universitas Indonesia, Depok 16424, IndonesiaLaboratory of Applied Genetic Engineering, Department of Plant Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, JapanLipopolysaccharide (LPS) is a major component of outer-membrane gram-negative bacteria, and it can act as a Pathogen-Associated Molecular Pattern (PAMP) for perception of pathogens by plants. LPS can be recognized by plants, triggering certain plant defense-related responses, including callose deposition. This study investigated induction of callose deposition by bacterial LPS in tobacco. Tobacco leaves were infiltrated with 400 μg/mL and 800 μg/mL LPS extracted from Pseudomonas syringae pv. tabaci (Pta) and Pseudomonas syringae pv. glycinea (Pgl) and incubated for 24 h or 48 h. To detect callose deposition, tobacco leaves were cleared in lactophenol solution, stained with aniline blue, and visualized by fluorescence microscopy. Results showed that LPS from Pgl induced more callose deposition in tobacco leaves than did that from Pta. In addition, a Pearson correlation test revealed that incubation period was the most significant factor in callose deposition, followed by the type of LPS bacteria. However, LPS concentration was not significantly corelated to callose deposition in tobacco leaves.http://journal.ui.ac.id/index.php/science/article/view/4343/3196callose depositionlipopolysaccharidePseudomonas syringae pv. tabaci Pseudomonas syringae. glycine; tobaccoPseudomonas syringae pv. tabaci Pseudomonas syringae. glycinetobacco |
| spellingShingle | Pipit Marianingsih Andi Salamah Yuki Ichinose Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea Makara Journal of Science callose deposition lipopolysaccharide Pseudomonas syringae pv. tabaci Pseudomonas syringae. glycine; tobacco Pseudomonas syringae pv. tabaci Pseudomonas syringae. glycine tobacco |
| title | Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea |
| title_full | Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea |
| title_fullStr | Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea |
| title_full_unstemmed | Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea |
| title_short | Induction of Callose Deposition in Tobacco (Nicotiana tabacum) by Bacterial Lipopolysaccharide Pseudomonas syringae pv. tabaci and Pseudomonas syringae pv. glycinea |
| title_sort | induction of callose deposition in tobacco nicotiana tabacum by bacterial lipopolysaccharide pseudomonas syringae pv tabaci and pseudomonas syringae pv glycinea |
| topic | callose deposition lipopolysaccharide Pseudomonas syringae pv. tabaci Pseudomonas syringae. glycine; tobacco Pseudomonas syringae pv. tabaci Pseudomonas syringae. glycine tobacco |
| url | http://journal.ui.ac.id/index.php/science/article/view/4343/3196 |
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