The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa
Abstract Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls. Semen samples containing a large amount of functionally and morphologically impaire...
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| Format: | Article |
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BMC
2022-08-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03408-z |
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| author | Maria Eberhardt Sylwia Prochowska Anna M. Duszewska Ann Van Soom Wanda Olech Wojciech Niżański |
| author_facet | Maria Eberhardt Sylwia Prochowska Anna M. Duszewska Ann Van Soom Wanda Olech Wojciech Niżański |
| author_sort | Maria Eberhardt |
| collection | DOAJ |
| description | Abstract Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls. Semen samples containing a large amount of functionally and morphologically impaired or dead spermatozoa have lower tolerance for cryopreservation process. Such samples are prone to involve damage acrosomes, to produce and release reactive oxygen which negatively affects proper function of spermatozoas. It is a good practice to select intact and viable gametes before subjecting the sample to cryopreservation to improve the efficiency of this process. The aim of this study was to assess the ability of Percoll® density gradient centrifugation in order to improve the quality of wisent spermatozoa after cryopreservation. Spermatozoa samples were analysed with computer-assisted semen analysis system and flow cytometry. Results Percoll® density gradient centrifugation resulted in increased percentage of motile spermatozoa, higher proportion of spermatozoa with normal morphology and proper functionality but also in a significant reduction of the total number of gametes. Nevertheless, the concentration of frozen spermatoza was still sufficient for obtaining a few complete insemination doses suggested for cattle from each epididymis. Conclusions While creating a high-quality genetic reserve, for in vitro fertilisation purposes, eliminating detritus and improving the overall quality of samples is more important than total number of spermatozoa. For these reasons, the achievement of higher post thaw quality of spermatozoa justifies the purification of samples by centrifugation in a Percoll® density gradient prior to the cryopreservation process. |
| first_indexed | 2024-04-11T22:35:36Z |
| format | Article |
| id | doaj.art-22f8f37fc23b42a3b2547aceffbc8e13 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-11T22:35:36Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-22f8f37fc23b42a3b2547aceffbc8e132022-12-22T03:59:13ZengBMCBMC Veterinary Research1746-61482022-08-0118111110.1186/s12917-022-03408-zThe influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoaMaria Eberhardt0Sylwia Prochowska1Anna M. Duszewska2Ann Van Soom3Wanda Olech4Wojciech Niżański5Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life SciencesDepartment of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life SciencesDepartment of Morphological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life SciencesDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, University of GhentDepartment of Animal Genetics and Conservation, Institute of Animal Sciences, Warsaw University of Life SciencesDepartment of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life SciencesAbstract Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls. Semen samples containing a large amount of functionally and morphologically impaired or dead spermatozoa have lower tolerance for cryopreservation process. Such samples are prone to involve damage acrosomes, to produce and release reactive oxygen which negatively affects proper function of spermatozoas. It is a good practice to select intact and viable gametes before subjecting the sample to cryopreservation to improve the efficiency of this process. The aim of this study was to assess the ability of Percoll® density gradient centrifugation in order to improve the quality of wisent spermatozoa after cryopreservation. Spermatozoa samples were analysed with computer-assisted semen analysis system and flow cytometry. Results Percoll® density gradient centrifugation resulted in increased percentage of motile spermatozoa, higher proportion of spermatozoa with normal morphology and proper functionality but also in a significant reduction of the total number of gametes. Nevertheless, the concentration of frozen spermatoza was still sufficient for obtaining a few complete insemination doses suggested for cattle from each epididymis. Conclusions While creating a high-quality genetic reserve, for in vitro fertilisation purposes, eliminating detritus and improving the overall quality of samples is more important than total number of spermatozoa. For these reasons, the achievement of higher post thaw quality of spermatozoa justifies the purification of samples by centrifugation in a Percoll® density gradient prior to the cryopreservation process.https://doi.org/10.1186/s12917-022-03408-zWisentSpermatozoaPercoll®FreezabilityCryopreservation |
| spellingShingle | Maria Eberhardt Sylwia Prochowska Anna M. Duszewska Ann Van Soom Wanda Olech Wojciech Niżański The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa BMC Veterinary Research Wisent Spermatozoa Percoll® Freezability Cryopreservation |
| title | The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa |
| title_full | The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa |
| title_fullStr | The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa |
| title_full_unstemmed | The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa |
| title_short | The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa |
| title_sort | influence of percoll r density gradient centrifugation before cryopreservation on the quality of frozen wisent bison bonasus epididymal spermatozoa |
| topic | Wisent Spermatozoa Percoll® Freezability Cryopreservation |
| url | https://doi.org/10.1186/s12917-022-03408-z |
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