Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse
Objective To explore the role of zinc finger BED domain-containing protein 3, a member of zinc finger domain protein superfamily, in cortex cerebral development through conventional Zbed3 knockout mouse. Methods In situ hybridization and immunofluorescence were used to detect the mRNA and protein...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-07-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2020-40-7-948.pdf |
| _version_ | 1797365700473389056 |
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| author | SUN Chang-jie, SHU Peng-cheng, PENG Xiao-zhong |
| author_facet | SUN Chang-jie, SHU Peng-cheng, PENG Xiao-zhong |
| author_sort | SUN Chang-jie, SHU Peng-cheng, PENG Xiao-zhong |
| collection | DOAJ |
| description | Objective To explore the role of zinc finger BED domain-containing protein 3, a member of zinc finger domain protein superfamily, in cortex cerebral development through conventional Zbed3 knockout mouse. Methods In situ hybridization and immunofluorescence were used to detect the mRNA and protein of Zbed3. Immunofluorescence was recruited to analyze the proliferation of neural progenitor cells in Zbed3 conventional knockout mouse. EdU labeling and immunofluorescence were used to analyze the neural position and layer fates in Zbed3 conven-tional knockout mouse. RT-qPCR and Western blot were used to detect the gene expression difference caused by Zbed3 knockout. Results Zbed3 mRNA and protein were not found in Zbed3 conventional knockout mouse; Knockout of Zbed3 failed to affect the proliferation of neural progenitor cells and the formation of neocortex layers. The expression of β-catenin was up-regulated in Zbed3 conventional knockout mouse. Conclusions Knockout of Zbed3 is not able to cause the apparent phenotype in cortex cerebral development. |
| first_indexed | 2024-03-08T16:52:41Z |
| format | Article |
| id | doaj.art-22f9197ef0a24cec99d53189211da4f5 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:52:41Z |
| publishDate | 2020-07-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-22f9197ef0a24cec99d53189211da4f52024-01-05T03:16:48ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-07-01407948954Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouseSUN Chang-jie, SHU Peng-cheng, PENG Xiao-zhong01. State Key Laboratory of Medical Molecular Biology, Department of Molecular and Biochemistry, Medical Primate Research Center, Neuroscience Center, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005; ;2. Institute of Medical Biology CAMS, Kunming 650118, ChinaObjective To explore the role of zinc finger BED domain-containing protein 3, a member of zinc finger domain protein superfamily, in cortex cerebral development through conventional Zbed3 knockout mouse. Methods In situ hybridization and immunofluorescence were used to detect the mRNA and protein of Zbed3. Immunofluorescence was recruited to analyze the proliferation of neural progenitor cells in Zbed3 conventional knockout mouse. EdU labeling and immunofluorescence were used to analyze the neural position and layer fates in Zbed3 conven-tional knockout mouse. RT-qPCR and Western blot were used to detect the gene expression difference caused by Zbed3 knockout. Results Zbed3 mRNA and protein were not found in Zbed3 conventional knockout mouse; Knockout of Zbed3 failed to affect the proliferation of neural progenitor cells and the formation of neocortex layers. The expression of β-catenin was up-regulated in Zbed3 conventional knockout mouse. Conclusions Knockout of Zbed3 is not able to cause the apparent phenotype in cortex cerebral development.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2020-40-7-948.pdfzbed3|cortex cerebral development|canonical wnt signaling pathway|β-catenin |
| spellingShingle | SUN Chang-jie, SHU Peng-cheng, PENG Xiao-zhong Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse Jichu yixue yu linchuang zbed3|cortex cerebral development|canonical wnt signaling pathway|β-catenin |
| title | Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse |
| title_full | Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse |
| title_fullStr | Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse |
| title_full_unstemmed | Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse |
| title_short | Role of ZBED3 in cortex cerebral development through conventional Zbed3 knockout mouse |
| title_sort | role of zbed3 in cortex cerebral development through conventional zbed3 knockout mouse |
| topic | zbed3|cortex cerebral development|canonical wnt signaling pathway|β-catenin |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2020-40-7-948.pdf |
| work_keys_str_mv | AT sunchangjieshupengchengpengxiaozhong roleofzbed3incortexcerebraldevelopmentthroughconventionalzbed3knockoutmouse |