Recombinant antigens for immunodiagnosis of cystic echinococcosis
<p>Three cDNAs, termed <it>EpC1, TPxEg</it> and <it>EgG5</it>, were isolated by immunoscreening from an <it>Echinococcus granulosus</it> cDNA library. The recombinant phages exhibited strong reactivity with sera from humans with confirmed cystic echinococcos...
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| Format: | Article |
| Language: | English |
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BMC
2004-01-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/74/m74.htm |
| _version_ | 1818807986055806976 |
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| author | Li Jun Zhang Wen-Bao McManus Donald P. |
| author_facet | Li Jun Zhang Wen-Bao McManus Donald P. |
| author_sort | Li Jun |
| collection | DOAJ |
| description | <p>Three cDNAs, termed <it>EpC1, TPxEg</it> and <it>EgG5</it>, were isolated by immunoscreening from an <it>Echinococcus granulosus</it> cDNA library. The recombinant phages exhibited strong reactivity with sera from humans with confirmed cystic echinococcosis (CE) and with sera from mice infected with <it>E. granulosus</it> oncospheres. The cDNAs were subcloned into a pET vector, expressed as fusion proteins tagged with GST and affinity purified against the GST tag. Of the three recombinant proteins, EpC1 achieved the highest performance for serodiagnosis of CE in Western blot analysis using a panel of clinically defined human sera to initially address the sensitivity and specificity of the molecules. The protein yielded an overall sensitivity of 92.2% and specificity of 95.6%, levels unprecedented taking into account the large panel of 896 human sera that were tested. The strategy used may also prove suitable for improved immunodiagnosis of other parasitic infections. |
| first_indexed | 2024-12-18T19:34:22Z |
| format | Article |
| id | doaj.art-22fb8c82336f4dd28f889e4d0ecba4d1 |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-18T19:34:22Z |
| publishDate | 2004-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-22fb8c82336f4dd28f889e4d0ecba4d12022-12-21T20:55:39ZengBMCBiological Procedures Online1480-92222004-01-0161677710.1251/bpo74Recombinant antigens for immunodiagnosis of cystic echinococcosisLi JunZhang Wen-BaoMcManus Donald P.<p>Three cDNAs, termed <it>EpC1, TPxEg</it> and <it>EgG5</it>, were isolated by immunoscreening from an <it>Echinococcus granulosus</it> cDNA library. The recombinant phages exhibited strong reactivity with sera from humans with confirmed cystic echinococcosis (CE) and with sera from mice infected with <it>E. granulosus</it> oncospheres. The cDNAs were subcloned into a pET vector, expressed as fusion proteins tagged with GST and affinity purified against the GST tag. Of the three recombinant proteins, EpC1 achieved the highest performance for serodiagnosis of CE in Western blot analysis using a panel of clinically defined human sera to initially address the sensitivity and specificity of the molecules. The protein yielded an overall sensitivity of 92.2% and specificity of 95.6%, levels unprecedented taking into account the large panel of 896 human sera that were tested. The strategy used may also prove suitable for improved immunodiagnosis of other parasitic infections.http://www.biologicalprocedures.com/bpo/arts/1/74/m74.htmEchinococcusRecombinant proteinsBlotting, Western |
| spellingShingle | Li Jun Zhang Wen-Bao McManus Donald P. Recombinant antigens for immunodiagnosis of cystic echinococcosis Biological Procedures Online Echinococcus Recombinant proteins Blotting, Western |
| title | Recombinant antigens for immunodiagnosis of cystic echinococcosis |
| title_full | Recombinant antigens for immunodiagnosis of cystic echinococcosis |
| title_fullStr | Recombinant antigens for immunodiagnosis of cystic echinococcosis |
| title_full_unstemmed | Recombinant antigens for immunodiagnosis of cystic echinococcosis |
| title_short | Recombinant antigens for immunodiagnosis of cystic echinococcosis |
| title_sort | recombinant antigens for immunodiagnosis of cystic echinococcosis |
| topic | Echinococcus Recombinant proteins Blotting, Western |
| url | http://www.biologicalprocedures.com/bpo/arts/1/74/m74.htm |
| work_keys_str_mv | AT lijun recombinantantigensforimmunodiagnosisofcysticechinococcosis AT zhangwenbao recombinantantigensforimmunodiagnosisofcysticechinococcosis AT mcmanusdonaldp recombinantantigensforimmunodiagnosisofcysticechinococcosis |