Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose
One of the ways to enhance the yield of bacterial cellulose (BC) is by using dynamic aeration and different-type bioreactors because the microbial producers are strict aerobes. But in this case, the BC quality tends to worsen. Here we have combined static culture with aeration in the biosynthesis of...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/23/4241 |
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| author | Nadezhda A. Shavyrkina Ekaterina A. Skiba Anastasia E. Kazantseva Evgenia K. Gladysheva Vera V. Budaeva Nikolay V. Bychin Yulia A. Gismatulina Ekaterina I. Kashcheyeva Galina F. Mironova Anna A. Korchagina Igor N. Pavlov Gennady V. Sakovich |
| author_facet | Nadezhda A. Shavyrkina Ekaterina A. Skiba Anastasia E. Kazantseva Evgenia K. Gladysheva Vera V. Budaeva Nikolay V. Bychin Yulia A. Gismatulina Ekaterina I. Kashcheyeva Galina F. Mironova Anna A. Korchagina Igor N. Pavlov Gennady V. Sakovich |
| author_sort | Nadezhda A. Shavyrkina |
| collection | DOAJ |
| description | One of the ways to enhance the yield of bacterial cellulose (BC) is by using dynamic aeration and different-type bioreactors because the microbial producers are strict aerobes. But in this case, the BC quality tends to worsen. Here we have combined static culture with aeration in the biosynthesis of BC by symbiotic <i>Medusomyces gisevii</i> Sa-12 for the first time. A new aeration method by feeding the air onto the growth medium surface is proposed herein. The culture was performed in a Binder-400 climate chamber. The study found that the air feed at a rate of 6.3 L/min allows a 25% increase in the BC yield. Moreover, this aeration mode resulted in BC samples of stable quality. The thermogravimetric and X-ray structural characteristics were retained: the crystallinity index in reflection and transmission geometries were 89% and 92%, respectively, and the allomorph Iα content was 94%. Slight decreases in the degree of polymerization (by 12.0% compared to the control―no aeration) and elastic modulus (by 12.6%) are not critical. Thus, the simple aeration by feeding the air onto the culture medium surface has turned out to be an excellent alternative to dynamic aeration. Usually, when the cultivation conditions, including the aeration ones, are changed, characteristics of the resultant BC are altered either, due to the sensitivity of individual microbial strains. In our case, the stable parameters of BC samples under variable aeration conditions are explained by the concomitant factors: the new efficient aeration method and the highly adaptive microbial producer―symbiotic <i>Medusomyces gisevii</i> Sa-12. |
| first_indexed | 2024-03-10T04:46:10Z |
| format | Article |
| id | doaj.art-22fc137454ae4b558f524203e6646bdd |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T04:46:10Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-22fc137454ae4b558f524203e6646bdd2023-11-23T02:55:05ZengMDPI AGPolymers2073-43602021-12-011323424110.3390/polym13234241Static Culture Combined with Aeration in Biosynthesis of Bacterial CelluloseNadezhda A. Shavyrkina0Ekaterina A. Skiba1Anastasia E. Kazantseva2Evgenia K. Gladysheva3Vera V. Budaeva4Nikolay V. Bychin5Yulia A. Gismatulina6Ekaterina I. Kashcheyeva7Galina F. Mironova8Anna A. Korchagina9Igor N. Pavlov10Gennady V. Sakovich11Bioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaBioconversion Laboratory, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), 659322 Biysk, RussiaOne of the ways to enhance the yield of bacterial cellulose (BC) is by using dynamic aeration and different-type bioreactors because the microbial producers are strict aerobes. But in this case, the BC quality tends to worsen. Here we have combined static culture with aeration in the biosynthesis of BC by symbiotic <i>Medusomyces gisevii</i> Sa-12 for the first time. A new aeration method by feeding the air onto the growth medium surface is proposed herein. The culture was performed in a Binder-400 climate chamber. The study found that the air feed at a rate of 6.3 L/min allows a 25% increase in the BC yield. Moreover, this aeration mode resulted in BC samples of stable quality. The thermogravimetric and X-ray structural characteristics were retained: the crystallinity index in reflection and transmission geometries were 89% and 92%, respectively, and the allomorph Iα content was 94%. Slight decreases in the degree of polymerization (by 12.0% compared to the control―no aeration) and elastic modulus (by 12.6%) are not critical. Thus, the simple aeration by feeding the air onto the culture medium surface has turned out to be an excellent alternative to dynamic aeration. Usually, when the cultivation conditions, including the aeration ones, are changed, characteristics of the resultant BC are altered either, due to the sensitivity of individual microbial strains. In our case, the stable parameters of BC samples under variable aeration conditions are explained by the concomitant factors: the new efficient aeration method and the highly adaptive microbial producer―symbiotic <i>Medusomyces gisevii</i> Sa-12.https://www.mdpi.com/2073-4360/13/23/4241static cultureaerationbacterial cellulose<i>Medusomyces gisevii</i> Sa-12 |
| spellingShingle | Nadezhda A. Shavyrkina Ekaterina A. Skiba Anastasia E. Kazantseva Evgenia K. Gladysheva Vera V. Budaeva Nikolay V. Bychin Yulia A. Gismatulina Ekaterina I. Kashcheyeva Galina F. Mironova Anna A. Korchagina Igor N. Pavlov Gennady V. Sakovich Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose Polymers static culture aeration bacterial cellulose <i>Medusomyces gisevii</i> Sa-12 |
| title | Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose |
| title_full | Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose |
| title_fullStr | Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose |
| title_full_unstemmed | Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose |
| title_short | Static Culture Combined with Aeration in Biosynthesis of Bacterial Cellulose |
| title_sort | static culture combined with aeration in biosynthesis of bacterial cellulose |
| topic | static culture aeration bacterial cellulose <i>Medusomyces gisevii</i> Sa-12 |
| url | https://www.mdpi.com/2073-4360/13/23/4241 |
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