An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice
<p>Abstract</p> <p>Background</p> <p>Plants respond to low temperature through an intricately coordinated transcriptional network. The <it>CBF/DREB</it>-regulated network of genes has been shown to play a prominent role in freeze-tolerance of <it>Arabi...
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| Format: | Article |
| Language: | English |
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BMC
2007-06-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/175 |
| _version_ | 1811281761335771136 |
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| author | Jia Yulin Bajic Vladimir B Mohanty Bijayalaxmi Ressom Habtom W Yun Kil-Young Cheng Chen Yun Song de los Reyes Benildo G |
| author_facet | Jia Yulin Bajic Vladimir B Mohanty Bijayalaxmi Ressom Habtom W Yun Kil-Young Cheng Chen Yun Song de los Reyes Benildo G |
| author_sort | Jia Yulin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Plants respond to low temperature through an intricately coordinated transcriptional network. The <it>CBF/DREB</it>-regulated network of genes has been shown to play a prominent role in freeze-tolerance of <it>Arabidopsis </it>through the process of cold acclimation (CA). Recent evidence also showed that the <it>CBF/DREB </it>regulon is not unique to CA but evolutionarily conserved between chilling-insensitive (temperate) and chilling-sensitive (warm-season) plants. In this study, the wide contrast in chilling sensitivity between indica and japonica rice was used as model to identify other regulatory clusters by integrative analysis of promoter architecture (<it>ab initio</it>) and gene expression profiles.</p> <p>Results</p> <p>Transcriptome analysis in chilling tolerant japonica rice identified a subset of 121 '<it>early response</it>' genes that were upregulated during the initial 24 hours at 10°C. Among this group were four transcription factors including <it>ROS-bZIP1 </it>and another larger sub-group with a common feature of having as1/ocs-like elements in their promoters. Cold-induction of <it>ROS-bZIP1 </it>preceded the induction of as1/ocs-like element-containing genes and they were also induced by exogenous H<sub>2</sub>O<sub>2 </sub>at ambient temperature. Coordinated expression patterns and similar promoter architectures among the '<it>early response</it>' genes suggest that they belong to a potential regulon (<it>ROS-bZIP – as1/ocs </it>regulatory module) that responds to elevated levels of ROS during chilling stress. Cultivar-specific expression signatures of the candidate genes indicate a positive correlation between the activity of the putative regulon and genotypic variation in chilling tolerance.</p> <p>Conclusion</p> <p>A hypothetical model of an ROS-mediated regulon (<it>ROS-bZIP – as1/ocs</it>) triggered by chilling stress was assembled in rice. Based on the current results, it appears that this regulon is independent of ABA and <it>CBF/DREB</it>, and that its activation has an important contribution in configuring the rapid responses of rice seedlings to chilling stress.</p> |
| first_indexed | 2024-04-13T01:39:26Z |
| format | Article |
| id | doaj.art-22fe38ecf8814cfcb38a00511080ea1f |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T01:39:26Z |
| publishDate | 2007-06-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-22fe38ecf8814cfcb38a00511080ea1f2022-12-22T03:08:13ZengBMCBMC Genomics1471-21642007-06-018117510.1186/1471-2164-8-175An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica riceJia YulinBajic Vladimir BMohanty BijayalaxmiRessom Habtom WYun Kil-YoungCheng ChenYun Songde los Reyes Benildo G<p>Abstract</p> <p>Background</p> <p>Plants respond to low temperature through an intricately coordinated transcriptional network. The <it>CBF/DREB</it>-regulated network of genes has been shown to play a prominent role in freeze-tolerance of <it>Arabidopsis </it>through the process of cold acclimation (CA). Recent evidence also showed that the <it>CBF/DREB </it>regulon is not unique to CA but evolutionarily conserved between chilling-insensitive (temperate) and chilling-sensitive (warm-season) plants. In this study, the wide contrast in chilling sensitivity between indica and japonica rice was used as model to identify other regulatory clusters by integrative analysis of promoter architecture (<it>ab initio</it>) and gene expression profiles.</p> <p>Results</p> <p>Transcriptome analysis in chilling tolerant japonica rice identified a subset of 121 '<it>early response</it>' genes that were upregulated during the initial 24 hours at 10°C. Among this group were four transcription factors including <it>ROS-bZIP1 </it>and another larger sub-group with a common feature of having as1/ocs-like elements in their promoters. Cold-induction of <it>ROS-bZIP1 </it>preceded the induction of as1/ocs-like element-containing genes and they were also induced by exogenous H<sub>2</sub>O<sub>2 </sub>at ambient temperature. Coordinated expression patterns and similar promoter architectures among the '<it>early response</it>' genes suggest that they belong to a potential regulon (<it>ROS-bZIP – as1/ocs </it>regulatory module) that responds to elevated levels of ROS during chilling stress. Cultivar-specific expression signatures of the candidate genes indicate a positive correlation between the activity of the putative regulon and genotypic variation in chilling tolerance.</p> <p>Conclusion</p> <p>A hypothetical model of an ROS-mediated regulon (<it>ROS-bZIP – as1/ocs</it>) triggered by chilling stress was assembled in rice. Based on the current results, it appears that this regulon is independent of ABA and <it>CBF/DREB</it>, and that its activation has an important contribution in configuring the rapid responses of rice seedlings to chilling stress.</p>http://www.biomedcentral.com/1471-2164/8/175 |
| spellingShingle | Jia Yulin Bajic Vladimir B Mohanty Bijayalaxmi Ressom Habtom W Yun Kil-Young Cheng Chen Yun Song de los Reyes Benildo G An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice BMC Genomics |
| title | An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice |
| title_full | An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice |
| title_fullStr | An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice |
| title_full_unstemmed | An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice |
| title_short | An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice |
| title_sort | early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling tolerant japonica rice |
| url | http://www.biomedcentral.com/1471-2164/8/175 |
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