Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68
<p>Abstract</p> <p>Background</p> <p>Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-speci...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-10-01
|
| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/11/451 |
| _version_ | 1818347807219646464 |
|---|---|
| author | Bedognetti Davide Di Pasquale Giovanni Roychoudhuri Rahul Pos Zoltan Chen Nanhai G Adams Sharon Reinboth Jennifer Yu Zhiya Worschech Andrea Ascierto Maria Uccellini Lorenzo Rossano Fabio Ascierto Paolo A Stroncek David F Restifo Nicholas P Wang Ena Szalay Aladar A Marincola Francesco M |
| author_facet | Bedognetti Davide Di Pasquale Giovanni Roychoudhuri Rahul Pos Zoltan Chen Nanhai G Adams Sharon Reinboth Jennifer Yu Zhiya Worschech Andrea Ascierto Maria Uccellini Lorenzo Rossano Fabio Ascierto Paolo A Stroncek David F Restifo Nicholas P Wang Ena Szalay Aladar A Marincola Francesco M |
| author_sort | Bedognetti Davide |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo.</p> <p>Methods</p> <p>In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection.</p> <p>Results</p> <p>We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection.</p> <p>Conclusions</p> <p>Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.</p> |
| first_indexed | 2024-12-13T17:40:02Z |
| format | Article |
| id | doaj.art-2304d4e709ad4366b0d29c3af47f8453 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-13T17:40:02Z |
| publishDate | 2011-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-2304d4e709ad4366b0d29c3af47f84532022-12-21T23:36:48ZengBMCBMC Cancer1471-24072011-10-0111145110.1186/1471-2407-11-451Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68Bedognetti DavideDi Pasquale GiovanniRoychoudhuri RahulPos ZoltanChen Nanhai GAdams SharonReinboth JenniferYu ZhiyaWorschech AndreaAscierto MariaUccellini LorenzoRossano FabioAscierto Paolo AStroncek David FRestifo Nicholas PWang EnaSzalay Aladar AMarincola Francesco M<p>Abstract</p> <p>Background</p> <p>Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo.</p> <p>Methods</p> <p>In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection.</p> <p>Results</p> <p>We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection.</p> <p>Conclusions</p> <p>Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.</p>http://www.biomedcentral.com/1471-2407/11/451 |
| spellingShingle | Bedognetti Davide Di Pasquale Giovanni Roychoudhuri Rahul Pos Zoltan Chen Nanhai G Adams Sharon Reinboth Jennifer Yu Zhiya Worschech Andrea Ascierto Maria Uccellini Lorenzo Rossano Fabio Ascierto Paolo A Stroncek David F Restifo Nicholas P Wang Ena Szalay Aladar A Marincola Francesco M Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68 BMC Cancer |
| title | Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68 |
| title_full | Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68 |
| title_fullStr | Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68 |
| title_full_unstemmed | Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68 |
| title_short | Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68 |
| title_sort | permissivity of the nci 60 cancer cell lines to oncolytic vaccinia virus glv 1h68 |
| url | http://www.biomedcentral.com/1471-2407/11/451 |
| work_keys_str_mv | AT bedognettidavide permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT dipasqualegiovanni permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT roychoudhurirahul permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT poszoltan permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT chennanhaig permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT adamssharon permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT reinbothjennifer permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT yuzhiya permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT worschechandrea permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT asciertomaria permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT uccellinilorenzo permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT rossanofabio permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT asciertopaoloa permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT stroncekdavidf permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT restifonicholasp permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT wangena permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT szalayaladara permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 AT marincolafrancescom permissivityofthenci60cancercelllinestooncolyticvacciniavirusglv1h68 |